Serologic and molecular survey for major viral pathogens in grazing hybrid wild boars in Northeast China

IMR OpenIR

	Serologic and molecular survey for major viral pathogens in grazing hybrid wild boars in Northeast China
其他题名	Serologic and molecular survey for major viral pathogens in grazing hybrid wild boars in Northeast China
	Guo Huancheng 1; Ren Zhaowen 1; Ding Meiming 2; Xiao Wanjun 1; Peng Peng 3; He Biao 1; Feng Ye 1; Liu Yan 1; Li Xingyu 1; Cai Jianqiu 1; Zhang Bikai 1; Luo Qinghua 1; Tu Changchun 1
	2019
发表期刊	JOURNAL OF INTEGRATIVE AGRICULTURE
ISSN	2095-3119
卷号	18 期号:9 页码:2133-2140
摘要	Hybrid wild boar husbandry is an important component of livestock production in Northeast China. However, the current disease situation of these animals is largely unknown due to a lack of disease surveillance. The present study was conducted to determine the prevalence of several important viral diseases in the hybrid wild boar population of Northeast China. Between September 2015 to December 2016, 169 blood and 61 tissue samples were collected from apparently healthy hybrid wild boars from farms in Jilin, Inner Mongolia and Heilongjiang provinces. ELISA detected serum antibodies against classical swine fever virus (CSFV), porcine reproductive and respiratory syndrome virus (PRRSV), pseudorabies virus (PRV), porcine circovirus type 2 (PCV2) and Japanese encephalitis virus (JEV), but not against African swine fever virus (ASFV), with PCV2 having the highest seropositive rate (87.2-100% in different farms). RT-PCR or PCR performed on the processed samples detected only PCV2, with 33.1% (56/169) of blood samples and 32.8% (20/61) of spleen samples being positive, respectively, indicating widespread PCV2 infection in hybrid wild boars. Phylogenetic analysis of 15 PCV2 ORF2 sequences showed that they belong to genotypes PCV2a, PCV2b and PCV2d, with nucleotide and deduced amino acid homologies of 88.5-100% and 88.1-100%, respectively.
其他摘要	Hybrid wild boar husbandry is an important component of livestock production in Northeast China. However, the current disease situation of these animals is largely unknown due to a lack of disease surveillance. The present study was conducted to determine the prevalence of several important viral diseases in the hybrid wild boar population of Northeast China. Between September 2015 to December 2016, 169 blood and 61 tissue samples were collected from apparently healthy hybrid wild boars from farms in Jilin, Inner Mongolia and Heilongjiang provinces. ELISA detected serum antibodies against classical swine fever virus (CSFV), porcine reproductive and respiratory syndrome virus (PRRSV), pseudorabies virus (PRV), porcine circovirus type 2 (PCV2) and Japanese encephalitis virus (JEV), but not against African swine fever virus (ASFV), with PCV2 having the highest seropositive rate (87.2–100% in different farms). RT-PCR or PCR performed on the processed samples detected only PCV2, with 33.1% (56/169) of blood samples and 32.8% (20/61) of spleen samples being positive, respectively, indicating widespread PCV2 infection in hybrid wild boars. Phylogenetic analysis of 15 PCV2 ORF2 sequences showed that they belong to genotypes PCV2a, PCV2b and PCV2d, with nucleotide and deduced amino acid homologies of 88.5–100% and 88.1–100%, respectively.
关键词	SWINE-FEVER VIRUS PORCINE CIRCOVIRUS TYPE-2 JAPANESE ENCEPHALITIS TRANSMISSION PSEUDORABIES EMERGENCE DISEASES disease transmission hybrid wild boars Northeast China serological and molecular survey viral diseases
收录类别	CSCD
语种	英语
资助项目	[National Key Research and Development Program of China] ; [Wildlife Borne Infectious Diseases Monitoring Project of the State Forestry Administration (SFA) of China]
CSCD记录号	CSCD:6579808
引用统计
文献类型	期刊论文
条目标识符	http://ir.imr.ac.cn/handle/321006/150455
专题	中国科学院金属研究所
作者单位	1.中山大学 2.Greater Xingan Mt Forestry Grp Corp, Div Wildlife Conservat & Nat Reserve Management, Jiagedaqi 165000, Peoples R China 3.中国科学院金属研究所 4.扬州大学
推荐引用方式 GB/T 7714	Guo Huancheng,Ren Zhaowen,Ding Meiming,et al. Serologic and molecular survey for major viral pathogens in grazing hybrid wild boars in Northeast China[J]. JOURNAL OF INTEGRATIVE AGRICULTURE,2019,18(9):2133-2140.
APA	Guo Huancheng.,Ren Zhaowen.,Ding Meiming.,Xiao Wanjun.,Peng Peng.,...&Tu Changchun.(2019).Serologic and molecular survey for major viral pathogens in grazing hybrid wild boars in Northeast China.JOURNAL OF INTEGRATIVE AGRICULTURE,18(9),2133-2140.
MLA	Guo Huancheng,et al."Serologic and molecular survey for major viral pathogens in grazing hybrid wild boars in Northeast China".JOURNAL OF INTEGRATIVE AGRICULTURE 18.9(2019):2133-2140.