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A fluorometric and mitochondrion-targetable probe for rapid, naked-eye test of hypochlorite in real samples
Xia Ying1; Liu Xiaoyu1; Wang Dan1; Wang Zechen1; Liu Qiang1; Yu Haibo1; Zhang Mingyan2; Song Youtao1
2018
Source PublicationCHINESE CHEMICAL LETTERS
ISSN1001-8417
Volume29Issue:10Pages:1517-1520
AbstractHypochlorite anion is a ubiquitous reactive molecule in the terminal disinfection systems, inflammatory stress and immune systems. Thus, rapid and visual monitoring ClO- in water and biological samples is very meaningful for water quality safety and toxicity assessment of contaminants. Herein, a colorimetric and fluorometric probe (Rh-GO) based on rhodamine B fluorophore and thiophene-2-carbohydrazide has been unveiled and successfully utilized for ClO- detection in water samples and HeLa cells. Upon addition of ClO-, color changes of solution from colorless to pink were immediately visible to the naked eyes, meanwhile, brilliant red fluorescence was observed under excited at UV light (365 nm). Rh-ClO displayed high selectivity and sensitivity for ClO-, and the detection limit was 7 mu mol/L calculated from the fluorescence titration. With the aid of its merits including rapid response to ClO- within 10 s, Rh-ClO and its test paper could successfully detect ClO- in water. Additionally, HeLa cells image co-stained with Rh-ClO and Rh123 demonstrated that Rh-ClO possessed excellent and fast cell-membrane permeability and mitochondrion-targetability. It was clearly confirmed that Rh-ClO would be a promising probe for rapid tracking of ClO- in water samples and in mitochondria of living cells. (C) 2018 Chinese Chemical Society and Institute of Materia Medica, Chinese Academy of Medical Sciences. Published by Elsevier B.V. All rights reserved.
Other AbstractHypochlorite anion is a ubiquitous reactive molecule in the terminal disinfection systems, inflammatory stress and immune systems. Thus, rapid and visual monitoring ClO~- in water and biological samples is very meaningful for water quality safety and toxicity assessment of contaminants. Herein, a colorimetric and fluorometric probe (Rh-ClO) based on rhodamine B fluorophore and thiophene-2-carbohydrazide has been unveiled and successfully utilized for ClO~- detection in water samples and HeLa cells. Upon addition of ClO~-, color changes of solution from colorless to pink were immediately visible to the nakedeyes, meanwhile, brilliant red fluorescence was observed under excited at UV light (365 nm). Rh-ClO displayed high selectivity and sensitivity for ClO~-, and the detection limit was 7mmol/L calculated from the fluorescence titration. With the aid of its merits including rapid response to ClO~- within 10 s, Rh-ClO and its test paper could successfully detect ClO~- inwater. Additionally, HeLa cells image co-stained with Rh-ClO and Rh123 demonstrated that Rh-ClO possessed excellent and fast cell-membrane permeability and mitochondrion-targetability. It was clearly confirmed that Rh-ClO would be a promising probe for rapid tracking of ClO~- in water samples and in mitochondria of living cells.
KeywordDISINFECTION BY-PRODUCTS SODIUM-HYPOCHLORITE FLUORESCENT-PROBE DRINKING-WATER PERACETIC-ACID WASTE-WATER OXIDATION MYELOPEROXIDASE CHEMOSENSORS GENOTOXICITY Colorimetric probe Fluorescent probe Rhodamine Hypochlorite Mitochondrion-tragetable
Indexed ByCSCD
Language英语
Funding Project[National Natural Science Foundation of China] ; [Liaoning Province Education Administration]
CSCD IDCSCD:6346038
Citation statistics
Cited Times:4[CSCD]   [CSCD Record]
Document Type期刊论文
Identifierhttp://ir.imr.ac.cn/handle/321006/151311
Collection中国科学院金属研究所
Affiliation1.辽宁大学
2.中国科学院金属研究所
Recommended Citation
GB/T 7714
Xia Ying,Liu Xiaoyu,Wang Dan,et al. A fluorometric and mitochondrion-targetable probe for rapid, naked-eye test of hypochlorite in real samples[J]. CHINESE CHEMICAL LETTERS,2018,29(10):1517-1520.
APA Xia Ying.,Liu Xiaoyu.,Wang Dan.,Wang Zechen.,Liu Qiang.,...&Song Youtao.(2018).A fluorometric and mitochondrion-targetable probe for rapid, naked-eye test of hypochlorite in real samples.CHINESE CHEMICAL LETTERS,29(10),1517-1520.
MLA Xia Ying,et al."A fluorometric and mitochondrion-targetable probe for rapid, naked-eye test of hypochlorite in real samples".CHINESE CHEMICAL LETTERS 29.10(2018):1517-1520.
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