A fluorometric and mitochondrion-targetable probe for rapid, naked-eye test of hypochlorite in real samples

IMR OpenIR

	A fluorometric and mitochondrion-targetable probe for rapid, naked-eye test of hypochlorite in real samples
	Xia Ying 1; Liu Xiaoyu 1; Wang Dan 1; Wang Zechen 1; Liu Qiang 1; Yu Haibo 1; Zhang Mingyan 2; Song Youtao 1
	2018
发表期刊	CHINESE CHEMICAL LETTERS
ISSN	1001-8417
卷号	29 期号:10 页码:1517-1520
摘要	Hypochlorite anion is a ubiquitous reactive molecule in the terminal disinfection systems, inflammatory stress and immune systems. Thus, rapid and visual monitoring ClO- in water and biological samples is very meaningful for water quality safety and toxicity assessment of contaminants. Herein, a colorimetric and fluorometric probe (Rh-GO) based on rhodamine B fluorophore and thiophene-2-carbohydrazide has been unveiled and successfully utilized for ClO- detection in water samples and HeLa cells. Upon addition of ClO-, color changes of solution from colorless to pink were immediately visible to the naked eyes, meanwhile, brilliant red fluorescence was observed under excited at UV light (365 nm). Rh-ClO displayed high selectivity and sensitivity for ClO-, and the detection limit was 7 mu mol/L calculated from the fluorescence titration. With the aid of its merits including rapid response to ClO- within 10 s, Rh-ClO and its test paper could successfully detect ClO- in water. Additionally, HeLa cells image co-stained with Rh-ClO and Rh123 demonstrated that Rh-ClO possessed excellent and fast cell-membrane permeability and mitochondrion-targetability. It was clearly confirmed that Rh-ClO would be a promising probe for rapid tracking of ClO- in water samples and in mitochondria of living cells. (C) 2018 Chinese Chemical Society and Institute of Materia Medica, Chinese Academy of Medical Sciences. Published by Elsevier B.V. All rights reserved.
其他摘要	Hypochlorite anion is a ubiquitous reactive molecule in the terminal disinfection systems, inflammatory stress and immune systems. Thus, rapid and visual monitoring ClO~- in water and biological samples is very meaningful for water quality safety and toxicity assessment of contaminants. Herein, a colorimetric and fluorometric probe (Rh-ClO) based on rhodamine B fluorophore and thiophene-2-carbohydrazide has been unveiled and successfully utilized for ClO~- detection in water samples and HeLa cells. Upon addition of ClO~-, color changes of solution from colorless to pink were immediately visible to the nakedeyes, meanwhile, brilliant red fluorescence was observed under excited at UV light (365 nm). Rh-ClO displayed high selectivity and sensitivity for ClO~-, and the detection limit was 7mmol/L calculated from the fluorescence titration. With the aid of its merits including rapid response to ClO~- within 10 s, Rh-ClO and its test paper could successfully detect ClO~- inwater. Additionally, HeLa cells image co-stained with Rh-ClO and Rh123 demonstrated that Rh-ClO possessed excellent and fast cell-membrane permeability and mitochondrion-targetability. It was clearly confirmed that Rh-ClO would be a promising probe for rapid tracking of ClO~- in water samples and in mitochondria of living cells.
关键词	DISINFECTION BY-PRODUCTS SODIUM-HYPOCHLORITE FLUORESCENT-PROBE DRINKING-WATER PERACETIC-ACID WASTE-WATER OXIDATION MYELOPEROXIDASE CHEMOSENSORS GENOTOXICITY Colorimetric probe Fluorescent probe Rhodamine Hypochlorite Mitochondrion-tragetable
收录类别	CSCD
语种	英语
资助项目	[National Natural Science Foundation of China] ; [Liaoning Province Education Administration]
CSCD记录号	CSCD:6346038
引用统计	被引频次：7[CSCD] [CSCD记录]
文献类型	期刊论文
条目标识符	http://ir.imr.ac.cn/handle/321006/151311
专题	中国科学院金属研究所
作者单位	1.辽宁大学 2.中国科学院金属研究所
推荐引用方式 GB/T 7714	Xia Ying,Liu Xiaoyu,Wang Dan,et al. A fluorometric and mitochondrion-targetable probe for rapid, naked-eye test of hypochlorite in real samples[J]. CHINESE CHEMICAL LETTERS,2018,29(10):1517-1520.
APA	Xia Ying.,Liu Xiaoyu.,Wang Dan.,Wang Zechen.,Liu Qiang.,...&Song Youtao.(2018).A fluorometric and mitochondrion-targetable probe for rapid, naked-eye test of hypochlorite in real samples.CHINESE CHEMICAL LETTERS,29(10),1517-1520.
MLA	Xia Ying,et al."A fluorometric and mitochondrion-targetable probe for rapid, naked-eye test of hypochlorite in real samples".CHINESE CHEMICAL LETTERS 29.10(2018):1517-1520.