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Analysis of the diversity and function of the alleles of the rice blast resistance genes Piz-t, Pita and Pik in 24 rice cultivars
Alternative TitleAnalysis of the diversity and function of the alleles of the rice blast resistance genes Piz-t, Pita and Pik in 24 rice cultivars
Wang Yan1; Zhao Jiaming1; Zhang Lixia1; Wang Ping1; Wang Shiwei2; Wang Hui1; Wang Xiaoxi1; Liu Zhiheng1; Zheng Wenjing1
2016
Source PublicationJOURNAL OF INTEGRATIVE AGRICULTURE
ISSN2095-3119
Volume15Issue:7Pages:1423-1431
AbstractUnderstanding the sequence diversity of rice blast resistance genes is important for breeding new resistant rice cultivars against the rice blast fungus Magnaporthe oryzae. In this study, we selected 24 rice cultivars with different genetic backgrounds to study the allelic diversity of rice blast resistance genes Piz-t, Pita and Pik. For Piz-t, a total of 17 allelic types were found within the 24 cultivars. Blast inoculations showed that most of the mutations can affect the function of the resistance gene. For Pita, except for the difference at the 918th amino acid, a majority of the 21 mutations were detected among the cultivars. Inoculations with blast isolates carrying Ayr-Pita revealed that cultivars with mutations in other sites except for the 918th amino acid did not affect the function of the Pita gene. For Pik, a total of six allelic types were found within the 24 cultivars, but five of them lost the function of the resistance gene. In addition, we found that Piz-t, Pita and Pik were expressed constitutively in the 24 rice cultivars and the expression level was not related to resistance. Our results have provided the sequence diversity information of the resistance genes Piz-t, Pita and Pik among the popular rice cultivars grown in the northeast region of China.
Other AbstractUnderstanding the sequence diversity of rice blast resistance genes is important for breeding new resistant rice cultivars against the rice blast fungus Magnaporthe oryzae. In this study, we selected 24 rice cultivars with different genetic backgrounds to study the allelic diversity of rice blast resistance genes Piz-t, Pita and Pik. For Piz-t, a total of 17 allelic types were found within the 24 cultivars. Blast inoculations showed that most of the mutations can affect the function of the resistance gene. For Pita, except for the difference at the 918th amino acid, a majority of the 21 mutations were detected among the cultivars. Inoculations with blast isolates carrying Avr-Pita revealed that cultivars with mutations in other sites except for the 918th amino acid did not affect the function of the Pita gene. For Pik, a total of six allelic types were found within the 24 cultivars, but five of them lost the function of the resistance gene. In addition, we found that Piz-t, Pita and Pik were expressed constitutively in the 24 rice cultivars and the expression level was not related to resistance. Our results have provided the sequence diversity information of the resistance genes Piz-t, Pita and Pik among the popular rice cultivars grown in the northeast region of China.
KeywordMAGNAPORTHE-ORYZAE ARMS-RACE IDENTIFICATION PROTEIN SEARCH PATHOSYSTEM STRATEGIES PROVINCE GRISEA resistance gene avirulence gene alleles function genetic evolution
Indexed ByCSCD
Language英语
Funding Project[National Natural Science Foundation of China] ; [Natural Science Foundation of Liaoning Province of China] ; [Doctoral Fund of Liaoning Province of China]
CSCD IDCSCD:5755906
Citation statistics
Document Type期刊论文
Identifierhttp://ir.imr.ac.cn/handle/321006/151394
Collection中国科学院金属研究所
Affiliation1.中国科学院金属研究所
2.China Agr University, Dept Plant Pathol, Beijing 100193, Peoples R China
Recommended Citation
GB/T 7714
Wang Yan,Zhao Jiaming,Zhang Lixia,et al. Analysis of the diversity and function of the alleles of the rice blast resistance genes Piz-t, Pita and Pik in 24 rice cultivars[J]. JOURNAL OF INTEGRATIVE AGRICULTURE,2016,15(7):1423-1431.
APA Wang Yan.,Zhao Jiaming.,Zhang Lixia.,Wang Ping.,Wang Shiwei.,...&Zheng Wenjing.(2016).Analysis of the diversity and function of the alleles of the rice blast resistance genes Piz-t, Pita and Pik in 24 rice cultivars.JOURNAL OF INTEGRATIVE AGRICULTURE,15(7),1423-1431.
MLA Wang Yan,et al."Analysis of the diversity and function of the alleles of the rice blast resistance genes Piz-t, Pita and Pik in 24 rice cultivars".JOURNAL OF INTEGRATIVE AGRICULTURE 15.7(2016):1423-1431.
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