Solid-phase synthesis of acyl-plasminogen-streptokinase activator composite(APSAC) and its thrombolytic properties

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	Solid-phase synthesis of acyl-plasminogen-streptokinase activator composite(APSAC) and its thrombolytic properties
其他题名	Solid-phase Synthesis of Acyl-plasminogen-Streptokinase Activator Composite(APSAC) and Its Thrombolytic Properties
	Teng LR 1; Liang F 1; Sun H 1; Qin Y 1; Hong SS 1; Chen J 1; Liu LY 1
	2004
发表期刊	CHEMICAL RESEARCH IN CHINESE UNIVERSITIES
ISSN	1005-9040
卷号	20 期号:5 页码:584-587
摘要	The dialysis method has been traditionally used for the conversion of native human plasminogen (Glu-Hpg) to lys-plasminogen(Lys-Hpg). Here is described a solid-phase synthesis method for. the preparation of an acyl-plasminogen-streptokinase activator complex (APSAC) from Lys-Hpg, streptokinase (SK) and chemical modification agent(4-amidinophenyl-4'-aminobenzoate hydrochloride) with the L-lysine-Sepharose 4B Column as the carrier. The new method significantly increases the product yield and purity over the liquid-phase methods. The APSAC prepared with the new method exhibits a significant thrombolytic effect with a long half-life of about 8.8 h in rabbits.
其他摘要	The dialysis method has been traditionally used for the conversion of native human plasminogen (GluHpg) to lys-plasminogen(Lys-Hpg). Here is described a solid-phase synthesis method for the preparation of an acyl-plasminogen-streptokinase activator complex(APSAC) from Lys-Hpg, streptokinase (SK) and chemical modification agent(4-amidinophenyl-4'-aminobenzoate hydrochloride) with the L-lysine-Sepharose 4B Column as the carrier. The new method significantly increases the product yield and purity over the liquidphase methods. The APSAC prepared with the new method exhibits a significant thrombolytic effect with a long half-life of about 8. 8 h in rabbits.
关键词	CONVERSION acyl-plasminogen-streptokinase activator complex (APSAC) solid-phase synthesis thrombolytic
收录类别	CSCD
语种	英语
CSCD记录号	CSCD:1844816
引用统计	被引频次：1[CSCD] [CSCD记录]
文献类型	期刊论文
条目标识符	http://ir.imr.ac.cn/handle/321006/151441
专题	中国科学院金属研究所
作者单位	1.吉林大学 2.Institute Biol Prod 3.中国科学院金属研究所
推荐引用方式 GB/T 7714	Teng LR,Liang F,Sun H,et al. Solid-phase synthesis of acyl-plasminogen-streptokinase activator composite(APSAC) and its thrombolytic properties[J]. CHEMICAL RESEARCH IN CHINESE UNIVERSITIES,2004,20(5):584-587.
APA	Teng LR.,Liang F.,Sun H.,Qin Y.,Hong SS.,...&Liu LY.(2004).Solid-phase synthesis of acyl-plasminogen-streptokinase activator composite(APSAC) and its thrombolytic properties.CHEMICAL RESEARCH IN CHINESE UNIVERSITIES,20(5),584-587.
MLA	Teng LR,et al."Solid-phase synthesis of acyl-plasminogen-streptokinase activator composite(APSAC) and its thrombolytic properties".CHEMICAL RESEARCH IN CHINESE UNIVERSITIES 20.5(2004):584-587.