| The cellular repressor of E1A-stimulated genes promotes human VSMCs differentiation in vitro mediated by RhoA and SRF | |
| Alternative Title | The Cellular Repressor of E1A-stimulated Genes Promotes Human VSMCs Differentiation In vitro Mediated by RhoA and SRF |
| Han YL1; Xu HM1; Yan CH1; Hu Y1; Kang J1; Liu HW1 | |
| 2006 | |
| Source Publication | PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS
 |
| ISSN | 1000-3282 |
| Volume | 33Issue:5Pages:438-445 |
| Abstract | To investigate the mechanism of the cellular repressor of E1A-stimulated genes (CREG) on the differentiation of vascular smooth muscle cells (VSMCs), the human internal thoracic artery cells (named HITASY cells) were stably infected with sense-CREG pLNCX(2)(+)/CREG and antisense-CREG pLXSN(-)/CREG retroviral vectors and positive clone was abtained by G418 selection. The cells infected with pLNCX2(+)/CREG take on the differentiated phenotype and overexpression of CREG can enhance SM alpha-actin expression accompanied with increase of the total RhoA and nuclear serum response factor (SRF) expression detected by immunofluorescence and Western blot. And treated with a selective Rho kinase inhibitor Y-27632, the expression of SM at-actin and nuclear SRF protein induced by CREG was effectively reduced. Meanwhile, the opposite results were observed in the CREG-depression HITASY cells. And immunoprecipitation assays indicate that as a secreted protein, CREG can be detected in the media of pLNCX2(+)/CREG cells and can interact with insulin-like growth factor II/mannose 6-phosphate receptor (M6P/IGF2R). Furthermore, that using okadaic acid (an inhibitor of the protein phosphatases 2A) to decrease the number of M6P/IGF2R at the cell surface can significantly inhibit the expression of total RhoA, nuclear SRF and SM a-actin induced by CREG-overexpression. Taken together, as a secreted protein, CREG can enhance the expression of SM alpha-actin by interaction with M6P/IGF2R and perform a pivotal role in the process of VSMCs differentiation and phenotype modulation. |
| Keyword | CELLS RECEPTOR repressor protein E1A phenotype cell vascular smooth muscle human |
| Indexed By | CSCD |
| Language | 英语 |
| CSCD ID | CSCD:2543256 |
| Citation statistics |
Cited Times:7[CSCD]
[CSCD Record]
|
| Document Type | 期刊论文 |
| Identifier | http://ir.imr.ac.cn/handle/321006/151451 |
| Collection | 中国科学院金属研究所 |
| Affiliation | 1.中国科学院金属研究所 2.中国科学院大连化学物理研究所 |
| Recommended Citation GB/T 7714 | Han YL,Xu HM,Yan CH,et al. The cellular repressor of E1A-stimulated genes promotes human VSMCs differentiation in vitro mediated by RhoA and SRF[J]. PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS,2006,33(5):438-445. |
| APA | Han YL,Xu HM,Yan CH,Hu Y,Kang J,&Liu HW.(2006).The cellular repressor of E1A-stimulated genes promotes human VSMCs differentiation in vitro mediated by RhoA and SRF.PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS,33(5),438-445. |
| MLA | Han YL,et al."The cellular repressor of E1A-stimulated genes promotes human VSMCs differentiation in vitro mediated by RhoA and SRF".PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS 33.5(2006):438-445. |
| Files in This Item: | There are no files associated with this item. | |||||
Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.
Edit Comment