Regulatory effects of nerve growth factor on SH2-B beta expression in the lung and primary afferent neurons of asthmatic mice

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	Regulatory effects of nerve growth factor on SH2-B beta expression in the lung and primary afferent neurons of asthmatic mice
其他题名	Regulatory effects of nerve growth factor on SH2-B beta expression in the lung and primary afferent neurons of asthmatic mice
	Qi Jinping; Fang Xiubin
	2008
发表期刊	NEURAL REGENERATION RESEARCH
ISSN	1673-5374
卷号	3 期号:10 页码:1132-1135
摘要	BACKGROUND: Several studies have demonstrated that SH2-B beta is over-expressed in the lung, C-7-T-5 spinal ganglia, and the corresponding spinal dorsal horn of asthmatic mice. SH2-B b expression has been shown to positively correlate with nerve growth factor (NGF) expression levels. This indicates that SH2-B beta in the presence of NGF, may participate ill asthmatic attacks.
其他摘要	BACKGROUND: Several studies have demonstrated that SH2-B β is over-expressed in the lung, C_7-T_5 spinal ganglia, and the corresponding spinal dorsal horn of asthmatic mice. SH2-Bβ expression has been shown to positively correlate with nerve growth factor (NGF) expression levels. This indicates that SH2-Bβ, in the presence of NGF, may participate in asthmatic attacks.OBJECTIVE: To observe the effects of anti-NGF on SH2-Bβ expression in primary afferent neurons (C_7-T_5 spinal ganglia and corresponding spinal dorsal horn) and in the lung to further investigate the regulatory effects of NGF on SH2-Bβexpression.DESIGN, TIME AND SETTING: A completely randomized block design experiment was performed at the Department of Neurobiology, China Medical University between March 2004 and July 2005.MATERIALS: Thirty-six male, BALB/c mice were included in this study. Ovalbumin solution was purchased from Sigma, USA. SH2-B 13 polyclonal antibody was provided by Santa Cruz, USA. Anti-NGF reagent was obtained from Wuhan Boster Bioengineering Co., Ltd., China.METHODS: Thirty-six mice were randomly and evenly divided into three groups: control, model, and anti-NGF. In the model group, asthma was induced by intraperitoneal injection and aerosol inhalation of ovalbumin solution. Mice in the anti-NGF group received anti-NGF through the nasal cavity 3 hours prior to aerosol inhalation. In the control group, mice were subjected to experimental procedures similar to the model group, except that ovalbumin solution was replaced by phosphate buffered saline (PBS).MAIN OUTCOME MEASURES: SH2-Bβ expression in primary afferent neurons (C_7-T_5 spinal ganglia and the corresponding spinal dorsal horn) and the lung was detected by immunohistochemistry and Western blot. Immunostaining intensity level of SH2-Bβ was analyzed using the MetaMorph image analysis system.RESULTS: Immunohistochemistry results revealed that the mean intensity of SH2-Bβ expression in the C_7-T_5 spinal ganglia, the corresponding spinal dorsal horn, and the lungs was significantly greater in the model group than in the control group (P < 0.01). However, expression was significantly less in the anti-NGF group, compared with the model group (P < 0.01). Western Blot results demonstrated that SH2-B β expression was significantly greater in the model group C_7-T_5 spinal ganglia and lung, compared with the control group (P < 0.01). However, expression was significantly less in the anti-NGF group, compared with the model group (P < 0.01).CONCLUSION: The present study showed that, in the primary afferent neurons and the lung, SH2-Bβ participated in asthmatic attack. Anti-NGF down-regulated Stt2-Bβ expression in C_7-T_5 spinal ganglia and the corresponding spinal dorsal horn, as well as the lung, of asthmatic mice. These results indicate that SH2-Bβ could be an important signaling molecule in mediating effects of NGF during asthmatic attack.
关键词	AIRWAY INFLAMMATION SERUM LEPTIN HYPERRESPONSIVENESS DIFFERENTIATION RECEPTOR ENERGY GENES SH2B1 asthma nerve growth factors SH2-B beta spinal cord spinal ganglia
收录类别	CSCD
语种	英语
CSCD记录号	CSCD:3446940
引用统计
文献类型	期刊论文
条目标识符	http://ir.imr.ac.cn/handle/321006/152004
专题	中国科学院金属研究所
作者单位	中国科学院金属研究所
推荐引用方式 GB/T 7714	Qi Jinping,Fang Xiubin. Regulatory effects of nerve growth factor on SH2-B beta expression in the lung and primary afferent neurons of asthmatic mice[J]. NEURAL REGENERATION RESEARCH,2008,3(10):1132-1135.
APA	Qi Jinping,&Fang Xiubin.(2008).Regulatory effects of nerve growth factor on SH2-B beta expression in the lung and primary afferent neurons of asthmatic mice.NEURAL REGENERATION RESEARCH,3(10),1132-1135.
MLA	Qi Jinping,et al."Regulatory effects of nerve growth factor on SH2-B beta expression in the lung and primary afferent neurons of asthmatic mice".NEURAL REGENERATION RESEARCH 3.10(2008):1132-1135.