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Response of linear and cyclic electron flux to moderate high temperature and high light stress in tomato
Alternative TitleResponse of linear and cyclic electron flux to moderate high temperature and high light stress in tomato
Lu Tao; Shi Jiewei; Sun Zhouping; Qi Mingfang; Liu Yufeng; Li Tianlai
2017
Source PublicationJOURNAL OF ZHEJIANG UNIVERSITY-SCIENCE B
ISSN1673-1581
Volume18Issue:7Pages:635-648
AbstractObjective: To evaluate the possible photoprotection mechanisms of cyclic and linear electron flux (CEF and LEF) under specific high temperature and high light (HH) stress. Methods: Six-leaf-stage tomato seedlings ("Liaoyuanduoli", n=160) were divided into four parts: Part 1, served as control under 25 degrees C, 500 mu mol/(m(2).s); Part 2, spayed with distilled water (H2O) under 35 degrees C, 1000 mu mol/(m(2).s) (HH); Part 3, spayed with 100 mu mol/L diuron (DCMU, CEF inhibitor) under HH; Part 4, spayed with 60 mu mol/L methyl viologen (MV, LEF inhibitor) under HH. Energy conversion, photosystem I (PSI), and PSII activity, and trans-thylakoid membrane proton motive force were monitored during the treatment of 5 d and of the recovering 10 d. Results: HH decreased photochemical reaction dissipation (P) and the maximal photochemical efficiency of PSII (F-v/F-m), and increased the excitation energy distribution coefficient of PSII (beta); DCMU and MV aggravated the partition imbalance of the excitation energy (gamma) and the photoinhibition degree. With prolonged DCMU treatment time, electron transport rate and quantum efficiency of PSI (ETRI and Y-I) significantly decreased whereas acceptor and donor side limitation of PSI (Y-NA and Y-ND) increased. MV led to a significant decline and accession of yield of regulated and non-regulated energy Y-NPQ and Y-NO, respectively. Membrane integrity and ATPase activity were reduced by HH stress, and DCMU and MV enhanced inhibitory actions. Conclusions: The protective effects of CEF and LEF were mediated to a certain degree by meliorations in energy absorption and distribution as well as by maintenance of thylakoid membrane integrity and ATPase activity.
Other AbstractObjective: To evaluate the possible photoprotection mechanisms of cyclic and linear electron flux (CEF and LEF) under specific high temperature and high light (HH) stress. Methods: Six-leaf-stage tomato seedlings (“Liaoyuanduoli”, n=160) were divided into four parts: Part 1, served as control under 25 ℃, 500 μmol/(m~2·s); Part 2, spayed with distilled water (H_2O) under 35 ℃, 1000 μmol/(m~2·s) (HH); Part 3, spayed with 100 μmol/L diuron (DCMU, CEF inhibitor) under HH; Part 4, spayed with 60 μmol/L methyl viologen (MV, LEF inhibitor) under HH. Energy conversion, photosystem I (PSI), and PSII activity, and trans-thylakoid membrane proton motive force were monitored during the treatment of 5 d and of the recovering 10 d. Results: HH decreased photochemical reaction dissipation (P) and the maximal photochemical efficiency of PSII (F_v/F_m), and increased the excitation energy distribution coefficient of PSII (β); DCMU and MV aggravated the partition imbalance of the excitation energy (γ) and the photoinhibition degree. With prolonged DCMU treatment time, electron transport rate and quantum efficiency of PSI (ETR_I and Y_I) significantly decreased whereas acceptor and donor side limitation of PSI (Y_(NA) and Y_(ND)) increased. MV led to a significant decline and accession of yield of regulated and non-regulated energy YNPQ and YNO, respectively. Membrane integrity and ATPase activity were reduced by HH stress, and DCMU and MV enhanced inhibitory actions. Conclusions: The protective effects of CEF and LEF were mediated to a certain degree by meliorations in energy absorption and distribution as well as by maintenance of thylakoid membrane integrity and ATPase activity.
KeywordCHLOROPLAST NAD(P)H DEHYDROGENASE PHOTOSYSTEM-I CHLOROPHYLL FLUORESCENCE SUBLETHAL CONCENTRATIONS PHYSIOLOGICAL-ROLE PROTON GRADIENT HEAT-STRESS DELTA-PSI FLOW PHOTOSYNTHESIS Photoinhibition Photoprotection Thylakoid membrane Tomato
Indexed ByCSCD
Language英语
Funding Project[China Agriculture Research System] ; [National Natural Science Foundation of China] ; [National Key Technologies R & D Program of China]
CSCD IDCSCD:6034514
Citation statistics
Cited Times:1[CSCD]   [CSCD Record]
Document Type期刊论文
Identifierhttp://ir.imr.ac.cn/handle/321006/152308
Collection中国科学院金属研究所
Affiliation中国科学院金属研究所
Recommended Citation
GB/T 7714
Lu Tao,Shi Jiewei,Sun Zhouping,et al. Response of linear and cyclic electron flux to moderate high temperature and high light stress in tomato[J]. JOURNAL OF ZHEJIANG UNIVERSITY-SCIENCE B,2017,18(7):635-648.
APA Lu Tao,Shi Jiewei,Sun Zhouping,Qi Mingfang,Liu Yufeng,&Li Tianlai.(2017).Response of linear and cyclic electron flux to moderate high temperature and high light stress in tomato.JOURNAL OF ZHEJIANG UNIVERSITY-SCIENCE B,18(7),635-648.
MLA Lu Tao,et al."Response of linear and cyclic electron flux to moderate high temperature and high light stress in tomato".JOURNAL OF ZHEJIANG UNIVERSITY-SCIENCE B 18.7(2017):635-648.
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