Comparative transcriptome analysis of shortened fruit mutant in woodland strawberry (Fragaria vesca) using RNA-Seq

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	Comparative transcriptome analysis of shortened fruit mutant in woodland strawberry (Fragaria vesca) using RNA-Seq
其他题名	Comparative transcriptome analysis of shortened fruit mutant in woodland strawberry (Fragaria vesca) using RNA-Seq
	Wang Shouming; Li Weijia; Liu Yuexue; Li He; Ma Yue; Zhang Zhihong
	2017
发表期刊	JOURNAL OF INTEGRATIVE AGRICULTURE
ISSN	2095-3119
卷号	16 期号:4 页码:828-844
摘要	Genes controlling fruit appearance determine fruit shape and size. In ethylmethane sulfonate (EMS)-mutagenized lines of Fragaria vesca accession Yellow Wonder (YW), two fruit shapes are observed: wild-type long fruit and mutated shortened fruit (s.f). In this study, we first characterized sf based on morphology, histology, cytology and physiology. The sf was identified as a gibberellin (GA)-deficient mutant, and four complementary DNA (cDNA) libraries separately constructed from flower buds and small green fruits of YW and sf were sequenced to comparatively analyze transcriptome differences. A total of 29 differentially expressed GA pathway genes were identified by comparisons between YW1 and sfl, and 28 differentially expressed GA pathway genes were identified between YW2 and sf2. In addition, the expression patterns of 45 differentially expressed genes were validated by quantificational real-time PCR (qRT-PCR), and the results were highly concordant with the RNA-Seq results. This transcriptome analysis provides valuable information for understanding the molecular mechanisms of fruit development of strawberry.
其他摘要	Genes controlling fruit appearance determine fruit shape and size. In ethylmethane sulfonate (EMS)-mutagenized lines of Fragaria vesca accession Yellow Wonder (YW), two fruit shapes are observed: wild-type long fruit and mutated shortened fruit (sf). In this study, we first characterized sf based on morphology, histology, cytology and physiology. The sf was identified as a gibberellin (GA)-deficient mutant, and four complementary DNA (cDNA) libraries separately constructed from flower buds and small green fruits of YW and sf were sequenced to comparatively analyze transcriptome differences. A total of 29 differentially expressed GA pathway genes were identified by comparisons between YW1 and sf1, and 28 differentially expressed GA pathway genes were identified between YW2 and sf 2. In addition, the expression patterns of 45 differentially expressed genes were validated by quantificational real-time PCR (qRT-PCR), and the results were highly concordant with the RNA-Seq results. This transcriptome analysis provides valuable information for understanding the molecular mechanisms of fruit development of strawberry.
关键词	DE-NOVO CHARACTERIZATION GIBBERELLIN BIOSYNTHESIS GREEN-REVOLUTION DEFICIENT MUTANT CELL-DIVISION TOMATO MUTANT GENE ARABIDOPSIS PROTEIN GENOME strawberry fruit shape transcriptome RNA-Seq differentially expressed gene
收录类别	CSCD
语种	英语
资助项目	[National Natural Science Foundation of China]
CSCD记录号	CSCD:5962859
引用统计	被引频次：4[CSCD] [CSCD记录]
文献类型	期刊论文
条目标识符	http://ir.imr.ac.cn/handle/321006/152646
专题	中国科学院金属研究所
作者单位	中国科学院金属研究所
推荐引用方式 GB/T 7714	Wang Shouming,Li Weijia,Liu Yuexue,et al. Comparative transcriptome analysis of shortened fruit mutant in woodland strawberry (Fragaria vesca) using RNA-Seq[J]. JOURNAL OF INTEGRATIVE AGRICULTURE,2017,16(4):828-844.
APA	Wang Shouming,Li Weijia,Liu Yuexue,Li He,Ma Yue,&Zhang Zhihong.(2017).Comparative transcriptome analysis of shortened fruit mutant in woodland strawberry (Fragaria vesca) using RNA-Seq.JOURNAL OF INTEGRATIVE AGRICULTURE,16(4),828-844.
MLA	Wang Shouming,et al."Comparative transcriptome analysis of shortened fruit mutant in woodland strawberry (Fragaria vesca) using RNA-Seq".JOURNAL OF INTEGRATIVE AGRICULTURE 16.4(2017):828-844.