| Effects of genistein on proliferation and cell cycle of salivary adenoid cystic carcinoma cells | |
| Alternative Title | Effects of Genistein on Proliferation and Cell Cycle of Salivary Adenoid Cystic Carcinoma Cells |
| Ma Jie1; Wang Jie2; Zhong Ming2; Wang Zhaoyuan2 | |
| 2007 | |
| Source Publication | CHINESE JOURNAL OF CANCER RESEARCH
 |
| ISSN | 1000-9604 |
| Volume | 19Issue:1Pages:64-67 |
| Abstract | Objective: To investigate the growth inhibiting effect of tyrosine protein kinase inhibitor, genistein, on human salivary adenoid cystic carcinoma SACC- 83 cell line in vitro, and its effects on the expression of CyclinB1 protein and cell cycle. Methods: Effects of genistein on the growth of SACC- 83 cells in vitro were measured with MTT assay. Cell cycle was detected with flow cytometry. The expressions of CyclinB1 and Cdk1 proteins were measured with Western blot method, and the results of protein expression were quantitatively analyzed by FluorChem V2.0 software. The results were statistically analyzed by SPSS11.5 software. Results: Genistein inhibited the cell proliferation in a dose- dependant and time- dependant manner. The genistein- treated SACC- 83 cells were arrested in the G2/ M phase and had lower contents of CyclinB1 and Cdk1 proteins compared with the control group. Conclusion: The growth inhibiting effect of genistein on SACC- 83 cells may be associated with the regulations of genistein on the CyclinB1 and Cdk1 protein expressions and the cell cycle. |
| Other Abstract | Objective: To investigate the growth inhibiting effect of tyrosine protein kinase inhibitor, genistein, on human salivary adenoid cystic carcinoma SACC-83 cell line in vitro, and its effects on the expression of CyclinB1 protein and cell cycle. Methods: Effects of genistein on the growth of SACC-83 cells in vitro were measured with MTT assay. Cell cycle was detected with flow cytometry. The expressions of CyclinB1 and Cdk1 proteins were measured with Western blot method, and the results of protein expression were quantitatively analyzed by FluorChem V2.0 software. The results were statistically analyzed by SPSS11.5 software. Results: Genistein inhibited the cell proliferation in a dose-dependant and time-dependant manner. The genistein-treated SACC-83 cells were arrested in the G2/M phase and had lower contents of CyclinB1 and Cdk1 proteins compared with the control group. Conclusion: The growth inhibiting effect of genistein on SACC-83 cells may be associated with the regulations of genistein on the CyclinB1 and Cdk1 protein expressions and the cell cycle. |
| Keyword | PROSTATE-CANCER CELLS APOPTOSIS INDUCTION GROWTH adenoid cystic carcinoma genistein CyclinB1 cell cycle |
| Indexed By | CSCD |
| Language | 英语 |
| CSCD ID | CSCD:2920371 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.imr.ac.cn/handle/321006/153283 |
| Collection | 中国科学院金属研究所 |
| Affiliation | 1.中国科学院金属研究所 2.中国科学院大连化学物理研究所 |
| Recommended Citation GB/T 7714 | Ma Jie,Wang Jie,Zhong Ming,et al. Effects of genistein on proliferation and cell cycle of salivary adenoid cystic carcinoma cells[J]. CHINESE JOURNAL OF CANCER RESEARCH,2007,19(1):64-67. |
| APA | Ma Jie,Wang Jie,Zhong Ming,&Wang Zhaoyuan.(2007).Effects of genistein on proliferation and cell cycle of salivary adenoid cystic carcinoma cells.CHINESE JOURNAL OF CANCER RESEARCH,19(1),64-67. |
| MLA | Ma Jie,et al."Effects of genistein on proliferation and cell cycle of salivary adenoid cystic carcinoma cells".CHINESE JOURNAL OF CANCER RESEARCH 19.1(2007):64-67. |
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