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Effects of ginsenoside on brain-derived neurotrophic factor and tyrosine kinase B mRNA expression in the hippocampal formation of aged rats
Alternative TitleEffects of ginsenoside on brain-derived neurotrophic factor and tyrosine kinase B mRNA expression in the hippocampal formation of aged rats
Lai Hong1; Liu Wensu2; Li Zhaosheng1; Zhao Haihua1; Lue Yongli1
2008
Source PublicationNEURAL REGENERATION RESEARCH
ISSN1673-5374
Volume3Issue:7Pages:723-727
AbstractBACKGROUND: There are a limited number of studies involving the effects of ginsenosides, the active component of ginseng, on expression of hippocampal TrkB mRNA in aged rats.
Other AbstractBACKGROUND: There are a limited number of studies involving the effects of ginsenosides, the active component of ginseng, on expression of hippocampal TrkB mRNA in aged rats.OBJECTIVE: To observe expression of brain-derived neurotrophic factor (BDNF) and tyrosine kinase B (TrkB) mRNA in the hippocampal formation of aged rats, as well as changes after ginsenoside administrated.DESIGN, TIME AND SETTING: A randomized, controlled experiment was performed at the Department of Anatomy, College of Basic Medical Sciences, China Medical University in March 2005.MATERIALS: A total of 39 female, Wistar rats were randomly divided into 3 groups (n = 13 each): young (3-5 months old), aged (27 months old), and ginsenoside group (received 25mg/kg/d ginsenoside in the drinking water between 17 and 27 months of age).METHODS: Following anesthesia, the rats were exsanguinated and perfused transcardially with chilled, heparinized, 0.9% saline. The brains were removed and post-fixed in 40 g/L paraformaldehyde/phosphate buffer for 20 minutes, and further incubated in 30% sucrose/phosphate buffer overnight.MAIN OUTCOME MEASURES: In situ hybridization, immunohistochemistry, and image analysis were used to investigate expression of BDNF and TrkB mRNA in the hippocampal formation.RESULTS: The expression levels of BDNF in the hippocampal CA_3 and CA_1 of aged rats was significantly less than the young group (t = 2.879, 1.814, 1.984, P < 0.05). BDNF expression was significantly greater in the dentate gyrus of the ginsenoside group, compared with the aging group (t = 1.943, P < 0.01). The expression of TrkB mRNA in the hippocampal CA_3, CA_1, and dentate gyrus of aged rats was less than the young group (t = 3.540, 3.629, 17.905, P < 0.01). TrkB mRNA expression in the CA_3 region and dentate gyrus of the ginsenoside group was significantly greater compared with the aging group (t = 1.293, 3.386, P < 0.05, 0.01).CONCLUSION: BDNF and TrkB mRNA expression in the hippocampal formation were reduced in the aged group. However, ginsenosides can increase BDNF and TrkB mRNA expression in the hippocampal formation.
KeywordSPINAL-CORD BDNF NEURONS CELLS TRKB MEMORY RG1 PROLIFERATION NEUROGENESIS PROGENITORS ginsenosides brain-derived neurotrophic factor tyrosine kinase B hippocampus
Indexed ByCSCD
Language英语
Funding Project[Liaoning Natural Science Foundation]
CSCD IDCSCD:3347343
Citation statistics
Document Type期刊论文
Identifierhttp://ir.imr.ac.cn/handle/321006/153378
Collection中国科学院金属研究所
Affiliation1.中国科学院金属研究所
2.China University Geosci, Sch Geophys & Informat Technol, Beijing 100083, Peoples R China
Recommended Citation
GB/T 7714
Lai Hong,Liu Wensu,Li Zhaosheng,et al. Effects of ginsenoside on brain-derived neurotrophic factor and tyrosine kinase B mRNA expression in the hippocampal formation of aged rats[J]. NEURAL REGENERATION RESEARCH,2008,3(7):723-727.
APA Lai Hong,Liu Wensu,Li Zhaosheng,Zhao Haihua,&Lue Yongli.(2008).Effects of ginsenoside on brain-derived neurotrophic factor and tyrosine kinase B mRNA expression in the hippocampal formation of aged rats.NEURAL REGENERATION RESEARCH,3(7),723-727.
MLA Lai Hong,et al."Effects of ginsenoside on brain-derived neurotrophic factor and tyrosine kinase B mRNA expression in the hippocampal formation of aged rats".NEURAL REGENERATION RESEARCH 3.7(2008):723-727.
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