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Cloning and characterization of an apolipoprotein C2 promoter in the mouse central nervous system
Alternative TitleCloning and characterization of an apolipoprotein C2 promoter in the mouse central nervous system ★
Li Zhaoyang; Du Bing; Li Shengyang; Lv Xiangchuan; Zhou Shenglai; Yu Yang; Wang Wei; Zheng Zhihong
2013
Source PublicationNEURAL REGENERATION RESEARCH
ISSN1673-5374
Volume8Issue:2Pages:156-U97
AbstractApolipoprotein C2 is an important member of the apolipoprotein C family, and is a potent activator of lipoprotein lipase. In the central nervous system, apolipoprotein C2 plays an important role in the catabolism of triglyceride-rich lipoproteins. Studies into the exact regulatory mechanism of mouse apolipoprotein C2 expression have not been reported. In this study, seven luciferase expression vectors, which contained potential mouse apolipoprotein C2 gene promoters, were constructed and co-transfected with pRL-TK into HEK293T cells to investigate apolipoprotein C2 promoter activity. Luciferase assays indicated that the apolipoprotein C2 promoter region was mainly located in the +104 bp to +470 bp region. The activity of the different lengths of apolipoprotein C2 promoter region varied. This staggered negative-positive-negative arrangement indicates the complex regulation of apolipoprotein C2 expression and provides important clues for elucidating the regulatory mechanism of apolipoprotein C2 gene transcription.
Other AbstractApolipoprotein C2 is an important member of the apolipoprotein C family, and is a potent activator of lipoprotein lipase. In the central nervous system, apolipoprotein C2 plays an important role in the catabolism of triglyceride-rich lipoproteins. Studies into the exact regulatory mechanism of mouse apolipoprotein C2 expression have not been reported. In this study, seven luciferase expression vectors, which contained potential mouse apolipoprotein C2 gene promoters, were constructed and co-transfected with pRL-TK into HEK293T cells to investigate apolipoprotein C2 promoter activity. Luciferase assays indicated that the apolipoprotein C2 promoter region was mainly located in the +104 bp to +470 bp region. The activity of the different lengths of apolipoprotein C2 promoter region varied. This staggered negative-positive-negative arrangement indicates the complex regulation of apolipoprotein C2 expression and provides important clues for elucidating the regulatory mechanism of apolipoprotein C2 gene transcription.
KeywordLIPOPROTEIN-LIPASE TRANSCRIPTION FACTORS GENETIC ASSOCIATION EXPRESSION JUN DEFICIENCY ACTIVATION NEURONS DISEASE METABOLISM neural regeneration basic research apolipoprotein C2 promoter dual-luciferase reporter assay transcriptional activity regulatory elements grants-supported paper neuroregeneration
Indexed ByCSCD
Language英语
Funding Project[National Natural Science Foundation of China] ; [Education Department of Liaoning Province]
CSCD IDCSCD:4753407
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Document Type期刊论文
Identifierhttp://ir.imr.ac.cn/handle/321006/153729
Collection中国科学院金属研究所
Affiliation中国科学院金属研究所
Recommended Citation
GB/T 7714
Li Zhaoyang,Du Bing,Li Shengyang,et al. Cloning and characterization of an apolipoprotein C2 promoter in the mouse central nervous system[J]. NEURAL REGENERATION RESEARCH,2013,8(2):156-U97.
APA Li Zhaoyang.,Du Bing.,Li Shengyang.,Lv Xiangchuan.,Zhou Shenglai.,...&Zheng Zhihong.(2013).Cloning and characterization of an apolipoprotein C2 promoter in the mouse central nervous system.NEURAL REGENERATION RESEARCH,8(2),156-U97.
MLA Li Zhaoyang,et al."Cloning and characterization of an apolipoprotein C2 promoter in the mouse central nervous system".NEURAL REGENERATION RESEARCH 8.2(2013):156-U97.
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