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In vitro assay of cytoskeleton nanomechanics as a tool for screening potential anticancer effects of natural plant extract, tubeimoside I on human hepatoma (HepG2) cells
Alternative TitleIn vitro assay of cytoskeleton nanomechanics as a tool for screening potential anticancer effects of natural plant extract, tubeimoside I on human hepatoma (HepG2) cells
Zhao HongBo1; Wang YaShu3; Jiang XueMei2; Shi XiaoHao1; Zhong HongZhe4; Wang YaJie5; Chen Jun2; Deng LinHong1
2013
Source PublicationCHINESE SCIENCE BULLETIN
ISSN1001-6538
Volume58Issue:21Pages:2576-2583
AbstractCytoskeleton nanomechanics characterizes cancer cell's physical behaviors such as how it spread and invade. For anticancer drug, cytoskeleton nanomechanics may be a target to inhibit invasiveness and metastasis of cancer cells. Therefore, in vitro assay of cytoskeleton nanomechanics may be used to evaluate the effects of potential anticancer drug on various cancer types. Here, we investigated the effects of tubeimoside I (TBMS I) on human hepatoma (HepG2) cells by using optical magnetic twisting cytometry, a well-established technique for measuring nanomechanics of the F-actin cytoskeleton. TBMS I is a natural compound extracted from a traditional Chinese herbal medicine, and is reported with antitumor effect. In this study, we demonstrated that the cytoskeleton stiffness (G') of HepG2 cells was affected by TBMS I. G' exhibited a typical power law with respect to the loading frequency (f), i.e. g similar to f (alpha) . The magnitude of G' and the value of exponent (alpha) of the HepG2 cells decreased consistently with the increase of concentration for TBMS I exposure. In addition, the HepG2 cells responded to TBMS I much faster than the normal liver (L-02) cells. Such alteration of cytoskeleton nanomechanics induced by TBMS I was reported for the first time, which was further corroborated by assays of F-actin cytoskeleton structure and cell migration. Taken together, these results suggest that in vitro assay of cytoskeleton nanomechanics may have a great potential as an additional tool in screening of anticancer drug candidates.
Other AbstractCytoskeleton nanomechanics characterizes cancer cell’s physical behaviors such as how it spread and invade. For anticancer drug, cytoskeleton nanomechanics may be a target to inhibit invasiveness and metastasis of cancer cells. Therefore, in vitro assay of cytoskeleton nanomechanics may be used to evaluate the effects of potential anticancer drug on various cancer types. Here, we investigated the effects of tubeimoside I (TBMS I) on human hepatoma (HepG2) cells by using optical magnetic twisting cytometry, a well-established technique for measuring nanomechanics of the Factin cytoskeleton. TBMS I is a natural compound extracted from a traditional Chinese herbal medicine, and is reported with antitumor effect. In this study, we demonstrated that the cytoskeleton stiffness (G) of HepG2 cells was affected by TBMS I. G′ exhibited a typical power law with respect to the loading frequency (f), i.e. G~f~α. The magnitude of G′ and the value of exponent (α) of the HepG2 cells decreased consistently with the increase of concentration for TBMS I exposure. In addition, the HepG2 cells responded to TBMS I much faster than the normal liver (L-02) cells. Such alteration of cytoskeleton nanomechanics induced by TBMS I was reported for the first time, which was further corroborated by assays of Factin cytoskeleton structure and cell migration. Taken together, these results suggest that in vitro assay of cytoskeleton nanomechanics may have a great potential as an additional tool in screening of anticancer drug candidates.
KeywordMUSCLE CELLS ACTIN CYTOTOXICITY APOPTOSIS HepG2 cells F-actin cytoskeleton nanomechanics tubeimoside I anticancer drug screening
Indexed ByCSCD
Language英语
Funding Project[National Natural Science Foundation of China] ; [Training Program for Hundreds of Distinguished Leading Scientists of Chongqing] ; [Chongqing Natural Science Foundation] ; [Fundamental Research Funds for the Central Universities]
CSCD IDCSCD:4877268
Citation statistics
Document Type期刊论文
Identifierhttp://ir.imr.ac.cn/handle/321006/154159
Collection中国科学院金属研究所
Affiliation1.Changzhou Univ, Institute Biomed Engn & Hlth Sci, Changzhou 213164, Peoples R China
2.重庆大学
3.Chinese Peoples Armed Police Forces, Xinjiang Prov Corps Hosp, Urumqi 830002, Peoples R China Xinjiang Prov Corps Hosp
4.中国科学院金属研究所
5.Chinese Peoples Liberat Army, Hosp 324, Chongqing 400020, Peoples R China Hosp 324
Recommended Citation
GB/T 7714
Zhao HongBo,Wang YaShu,Jiang XueMei,et al. In vitro assay of cytoskeleton nanomechanics as a tool for screening potential anticancer effects of natural plant extract, tubeimoside I on human hepatoma (HepG2) cells[J]. CHINESE SCIENCE BULLETIN,2013,58(21):2576-2583.
APA Zhao HongBo.,Wang YaShu.,Jiang XueMei.,Shi XiaoHao.,Zhong HongZhe.,...&Deng LinHong.(2013).In vitro assay of cytoskeleton nanomechanics as a tool for screening potential anticancer effects of natural plant extract, tubeimoside I on human hepatoma (HepG2) cells.CHINESE SCIENCE BULLETIN,58(21),2576-2583.
MLA Zhao HongBo,et al."In vitro assay of cytoskeleton nanomechanics as a tool for screening potential anticancer effects of natural plant extract, tubeimoside I on human hepatoma (HepG2) cells".CHINESE SCIENCE BULLETIN 58.21(2013):2576-2583.
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