cDNA cloning and expression pattern of two enolase genes from the Chinese oak silkworm, Antheraea pernyi

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	cDNA cloning and expression pattern of two enolase genes from the Chinese oak silkworm, Antheraea pernyi
其他题名	Cdna cloning and expression pattern of two enolase genes from the chinese oak silkworm, antheraea pernyi
	Liu Yanqun 1; Li Yuping 1; Wang Huan 1; Xia Runxi 1; Li Xisheng 2; Wan Haolei 3; Qin Li 1; Jiang Defu 2; Lu Cheng 5; Xiang Zhonghuai 5
	2010
发表期刊	ACTA BIOCHIMICA ET BIOPHYSICA SINICA
ISSN	1672-9145
卷号	42 期号:11 页码:816-826
摘要	In this study, two enolase genes were isolated and characterized from the Chinese oak silkworm, Antheraea perny, which were designated as enolase I and II, respectively. The enolase I cDNA sequence was 1712 bp with an open reading frame (ORF) of 1302 bp encoding 433 amino acids. The enolase II cDNA sequence was 1549 bp with an ORF of 1296 bp encoding 431 amino acids. The amino acid sequences of the two genes share several conserved features/sites of enolase. Antheraea pernyi enolase I shows 93%-97% sequence identity to enolases of lepidopterans available to date, 75%-82% identity to enolases of other invertebrates, 60%-72% identity to enolases of other organisms including vertebrates, plants, and fungi. Antheraea pernyi enolase II shows 84% identity to Bombyx mori enolase II, but 60% identity to A. pernyi enolase I. In the phylogenetic tree, enolase II sequences from A. pernyi and B. mori were clearly separated from the majority of enolase sequences of higher organisms including A. pernyi and B. mori enolase I sequences. By sequence comparisons and phylogenetic analysis, we suggest that enolase II from A. pernyi and B. mori may be a new member of the enolase superfamily. Antheraea pernyi enolase I mRNA was found in all tested tissues whereas enolase II mRNA was expressed specifically in the spermaries and ovaries, suggesting that the product of enolase II gene may be related to reproduction. The transcript abundance of A. pernyi enolase I gene was significantly down-regulated after cold shock and significantly up-regulated after heat shock, suggesting that A. pernyi enolase I gene may be inducible by temperature stress.
其他摘要	In this study, two enolase genes were isolated and characterized from the chinese oak silkworm, antheraea perny, which were designated as enolase i and ii, respectively. the enolase i cdna sequence was 1712 bp with an open reading frame (orf) of 1302 bp encoding 433 amino acids. the enolase ii cdna sequence was 1549 bp with an orf of 1296 bp encoding 431 amino acids. the amino acid sequences of the two genes share several conserved features/sites of enolase. antheraea pernyi enolase i shows 93%-97% sequence identity to enolases of lepidopterans available to date, 75%-82% identity to enolases of other invertebrates, 60%-72% identity to enolases of other organisms including vertebrates, plants, and fungi. antheraea pernyi enolase ii shows 84% identity to bombyx mori enolase ii, but 60% identity to a. pernyi enolase i. in the phylogenetic tree, enolase ii sequences from a. pernyi and b. mori were clearly separated from the majority of enolase sequences of higher organisms including a. pernyi and b. mori enolase i sequences. by sequence comparisons and phylogenetic analysis, we suggest that enolase ii from a. pernyi and b. mori may be a new member of the enolase superfamily. antheraea pernyi enolase i mrna was found in all tested tissues whereas enolase ii mrna was expressed specifically in the spermaries and ovaries, suggesting that the product of enolase ii gene may be related to reproduction. the transcript abundance of a. pernyi enolase i gene was significantly down-regulated after cold shock and significantly up-regulated after heat shock, suggesting that a. pernyi enolase i gene may be inducible by temperature stress.
关键词	HUMAN ALPHA-ENOLASE NUCLEOTIDE-SEQUENCE MOLECULAR-CLONING GENOME SEQUENCE ANGSTROM RESOLUTION CRYSTAL-STRUCTURE BOMBYX-MORI PROTEIN EVOLUTION STRESS Antheraea pernyi enolase cloning expression pattern
收录类别	CSCD
语种	英语
资助项目	[National Basic Research Program of China] ; [National Natural Science Foundation of China] ; [National Modern Agriculture Industry Technology System] ; [Educational Department of Liaoning Province]
CSCD记录号	CSCD:4052642
引用统计
文献类型	期刊论文
条目标识符	http://ir.imr.ac.cn/handle/321006/154336
专题	中国科学院金属研究所
作者单位	1.中国科学院金属研究所 2.Sericultural Institute Liaoning Prov, Fengcheng 118100, Peoples R China 3.中国科学院 4.中国科学院大学 5.西南大学
推荐引用方式 GB/T 7714	Liu Yanqun,Li Yuping,Wang Huan,et al. cDNA cloning and expression pattern of two enolase genes from the Chinese oak silkworm, Antheraea pernyi[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2010,42(11):816-826.
APA	Liu Yanqun.,Li Yuping.,Wang Huan.,Xia Runxi.,Li Xisheng.,...&Xiang Zhonghuai.(2010).cDNA cloning and expression pattern of two enolase genes from the Chinese oak silkworm, Antheraea pernyi.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,42(11),816-826.
MLA	Liu Yanqun,et al."cDNA cloning and expression pattern of two enolase genes from the Chinese oak silkworm, Antheraea pernyi".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 42.11(2010):816-826.