IMR OpenIR
Regulation of demethylation and re-expression of RASSF1A gene in gastric cancer cell lines by combined treatment of 5-Aza-CdR and NaB
Alternative TitleRegulation of demethylation and re-expression of RASSF1A gene in gastric cancer cell lines by combined treatment of 5-Aza-CdR and NaB
Shen WenJing; Dai DongQiu; Teng Yue; Liu HongBo
2008
Source PublicationWORLD JOURNAL OF GASTROENTEROLOGY
ISSN1007-9327
Volume14Issue:4Pages:595-600
AbstractAIM: To investigate the changes of methylation state and expression of RASSF1A gene in human gastric cancer cell lines SGC7901 and BGC823 which were treated in vitro with demethlylating agent 5-Aza-CdR in combination with histone deacetylase inhibitor NaB.
Other AbstractAIM: To investigate the changes of methylation state and expression of RASSF1A gene in human gastric cancer cell lines SGC7901 and BGC823 which were treated in vitro with demethlylating agent 5-Aza-CdR in combination with histone deacetylase inhibitor NaB.METHODS: After SGC7901 and BGC823 cells were treated with 5-Aza-CdR and/or NaB, the methylation state of RASSF1A gene was detected by methylation-specific PCR, and the changes in expression of mRNA and protein level of RASSF1A gene were observed by RT-PCR and Western-blotting before and after drug treatment.RESULTS: Hypermethylation was detected in the promoter region of RASSF1A gene in both SGC7901 and BGC823 cells, and there was no expression of this gene at both mRNA and protein level. After treatment with 5-Aza-CdR, demethylation occurred in the promoter region of RASSF1A gene, which subsequently induced re-expression of this gene. The treatment with NaB alone showed no effect on the methylation state and expression of RASSF1A gene. The combined treatment of 5-Aza-CdR and NaB induced complete demethylation of RASSF1A gene, leading to a significantly higher re-expression of the mRNA and protein of RASSF1A than those treated with 5-Aza-CdR alone (P < 0.05).CONCLUSION: Hypermethylation in the promoter region is related to inactivation of RASSF1A gene in human gastric cancer cell lines SGC7901 and BGC823, while demethlylating agent 5-Aza-CdR can reverse the methylation state of RASSF1A gene and induce itsre-expression. Histone deacetylase inhibitor NaB had a synergistic effect with 5-Aza-CdR in both demethylation and gene transcriptional regulation.(c) 2008 WJG. All rights reserved.
KeywordDNA METHYLTRANSFERASE INHIBITORS HISTONE DEACETYLASE INHIBITION CYCLE PROGRESSION METHYLATION 5-AZA-2'-DEOXYCYTIDINE EXPRESSION TUMOR HYPERMETHYLATION ACETYLATION CARCINOGENESIS 5-Aza-CdR NaB RASSF1A gene gastric cancer DNA methylation
Indexed ByCSCD
Language英语
CSCD IDCSCD:3317134
Citation statistics
Document Type期刊论文
Identifierhttp://ir.imr.ac.cn/handle/321006/154534
Collection中国科学院金属研究所
Affiliation中国科学院金属研究所
Recommended Citation
GB/T 7714
Shen WenJing,Dai DongQiu,Teng Yue,et al. Regulation of demethylation and re-expression of RASSF1A gene in gastric cancer cell lines by combined treatment of 5-Aza-CdR and NaB[J]. WORLD JOURNAL OF GASTROENTEROLOGY,2008,14(4):595-600.
APA Shen WenJing,Dai DongQiu,Teng Yue,&Liu HongBo.(2008).Regulation of demethylation and re-expression of RASSF1A gene in gastric cancer cell lines by combined treatment of 5-Aza-CdR and NaB.WORLD JOURNAL OF GASTROENTEROLOGY,14(4),595-600.
MLA Shen WenJing,et al."Regulation of demethylation and re-expression of RASSF1A gene in gastric cancer cell lines by combined treatment of 5-Aza-CdR and NaB".WORLD JOURNAL OF GASTROENTEROLOGY 14.4(2008):595-600.
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Shen WenJing]'s Articles
[Dai DongQiu]'s Articles
[Teng Yue]'s Articles
Baidu academic
Similar articles in Baidu academic
[Shen WenJing]'s Articles
[Dai DongQiu]'s Articles
[Teng Yue]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Shen WenJing]'s Articles
[Dai DongQiu]'s Articles
[Teng Yue]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.