Effects of basic fibroblast growth factor on beta-catenin protein and mRNA expression during the proliferation of endogenous neural stem cells following focal cerebral ischemia

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	Effects of basic fibroblast growth factor on beta-catenin protein and mRNA expression during the proliferation of endogenous neural stem cells following focal cerebral ischemia
其他题名	Effects of basic fibroblast growth factor on beta-catenin protein and mRNA expression during the proliferation of endogenous neural stem cells following focal cerebral ischemia
	Xing Xuesong 1; Lug Weili 2; Sun Liguang 1
	2009
发表期刊	NEURAL REGENERATION RESEARCH
ISSN	1673-5374
卷号	4 期号:2 页码:111-117
摘要	BACKGROUND: The Wnt/ beta -catenin signaling pathway plays an important role in neural development. beta -catenin is an important component of the Wnt/ beta -catenin signaling pathway. The Wnt signaling pathway has been shown to regulate the interaction of neural stem cells with the extracellular matrix.
其他摘要	BACKGROUND: The Wnt/β -catenin signaling pathway plays an important role in neural development. Β-catenin is an important component of the Wnt/β -catenin signaling pathway. The Wnt signaling pathway has been shown to regulate the interaction of neural stem cells with the extracellular matrix.OBJECTIVE: To investigate the effects of basic fibroblast growth factor (bFGF) on β-catenin protein and mRNA expression, and on hippocampal neural stem cell proliferation in a rat model of cerebral ischemia/reperfusion.DESIGN, TIME AND SETTING: A randomized, controlled, neurobiology experiment was performed in Shenyang Medical College between August 2006 and August 2008.MATERIALS: A total of 72 healthy male Wistar rats, aged 3 months, were used in this study. bFGF was provided by Beijing SL Pharmaceutical Co.,Ltd., China.METHODS: Rats were randomly divided into 3 groups: sham-operated, ischemia/reperfusion, and bFGF-treated (n = 24 per group). Focal cerebral ischemia/reperfusion was induced in rats from the ischemia/reperfusion group and the bFGF-treated group by 2 hour right middle cerebral artery occlusion and 2 hour restoration of blood flow using the suture method. The ischemia/reperfusion and bFGF-treated groups were intraperitoneally administered 500 IU/mL of bFGF, or the same volume of physiological saline, once a day at postoperative days 1-3, and once every 3 days thereafter. Simultaneously, the sham-operated group underwent experimental procedures identical to the ischemia/reperfusion and bFGF-treated groups, with the exception of ischemia/reperfusion induction and drug administration. At 2 hours, 2, 6, 13, and 20 days after ischemia/reperfusion induction, 50 mg/kg bromodeoxyuridine (BrdU) was administered to each group, twice daily, to label proliferating neural stem cells.MAIN OUTCOME MEASURES: The effects of bFGF on BrdU labeling, and β-catenin mRNA and protein expression, in neural stem cells were examined by immunohistochemistry, Western blot, RT-PCR, and in situ hybridization techniques.RESULTS: In the sham-operated group, only a few BrdU-immunoreactive neural stem cells were found. In the ischemia/repeffusion group, BrdU-immunoreactive cells began to increase from 3 days after ischemia/reperfusion induction, reached a peak level at 7 days, and gradually reduced from 21 days. At 3, 7, 14, and 21 days after ischemia/reperfusion induction, the numbers of BrdU-immunoreactive cells were significantly greater in the bFGF-treated group than in the ischemia/reperfusion group. The sham-operated group exhibited slight expression of β-catenin and β-catenin mRNA. In the ischernia/reperfusion group, the expression of β-catenin and β-catenin mRNA gradually increased with reperfusion time, peaked at 14 days after reperfusion, and gradually decreased thereafter; by 21 days, the expression was markedly lower. Following bFGF injection, the expression of hippocampal BrdU, β -catenin, and β-catenin mRNA had apparently increased in each group.CONCLUSION: bFGF promotes neural stem cell proliferation, and the expression of β-catenin and β-catenin mRNA in the ischernic brain tissue. These findings indicate that bFGF promotion of neural stem cell proliferation may be mediated by Wnt/β-catenin signaling pathway.
关键词	HIPPOCAMPAL SLICE CULTURES DENTATE GYRUS SUBVENTRICULAR ZONE INCREASES NEUROGENESIS ADULT NEUROGENESIS RAT HIPPOCAMPUS SPATIAL MEMORY INFARCT VOLUME BFGF STROKE basic fibroblast growth factor beta -catenin neural stem cell proliferation rats
收录类别	CSCD
语种	英语
资助项目	[Scientific Research Foundation for Colleges and Universities of Education Department of Liaoning Province]
CSCD记录号	CSCD:3632143
引用统计	被引频次：1[CSCD] [CSCD记录]
文献类型	期刊论文
条目标识符	http://ir.imr.ac.cn/handle/321006/154783
专题	中国科学院金属研究所
作者单位	1.中国科学院地质与地球物理研究所 2.中国科学院金属研究所
推荐引用方式 GB/T 7714	Xing Xuesong,Lug Weili,Sun Liguang. Effects of basic fibroblast growth factor on beta-catenin protein and mRNA expression during the proliferation of endogenous neural stem cells following focal cerebral ischemia[J]. NEURAL REGENERATION RESEARCH,2009,4(2):111-117.
APA	Xing Xuesong,Lug Weili,&Sun Liguang.(2009).Effects of basic fibroblast growth factor on beta-catenin protein and mRNA expression during the proliferation of endogenous neural stem cells following focal cerebral ischemia.NEURAL REGENERATION RESEARCH,4(2),111-117.
MLA	Xing Xuesong,et al."Effects of basic fibroblast growth factor on beta-catenin protein and mRNA expression during the proliferation of endogenous neural stem cells following focal cerebral ischemia".NEURAL REGENERATION RESEARCH 4.2(2009):111-117.