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Biofunctional magnesium coated Ti6Al4V scaffold enhances osteogenesis and angiogenesis in vitro and in vivo for orthopedic application
Gao, Peng; Fan, Bo; Yu, Xiaoming; Liu, Wenwen; Wu, Jie; Shi, Lei; Yang, Di; Tan, Lili; Wan, Peng; Hao, Yulin; Li, Shujun; Hou, Wentao; Yang, Ke; Li, Xiaokang; Guo, Zheng
2020-09-01
Source PublicationBIOACTIVE MATERIALS
Volume5Issue:3Pages:680-693
AbstractThe insufficient osteogenesis and osseointegration of porous titanium based scaffold limit its further application. Early angiogenesis is important for scaffold survival. It is necessary to develop a multifunctional surface on titanium scaffold with both osteogenic and angiogenic properties. In this study, a biofunctional magnesium coating is deposited on porous Ti6Al4V scaffold. For osseointegration and osteogenesis analysis, in vitro studies reveal that magnesium-coated Ti6Al4V co-culture with MC3T3-E1 cells can improve cell proliferation, adhesion, extracellular matrix (ECM) mineralization and ALP activity compared with bare Ti6Al4V cocultivation. Additionally, MC3T3-E1 cells cultured with magnesium-coated Ti6Al4V show significantly higher osteogenesis-related genes expression. In vivo studies including fluorochrome labeling, micro-computerized tomography and histological examination of magnesium-coated Ti6Al4V scaffold reveal that new bone regeneration is significantly increased in rabbits after implantation. For angiogenesis studies, magnesium-coated Ti6Al4V improve HUVECs proliferation, adhesion, tube formation, wound-healing and Transwell abilities. HUVECs cultured with magnesium-coated Ti6Al4V display significantly higher angiogenesis-related genes (HIF-1 alpha and VEGF) expression. Microangiography analysis reveal that magnesium-coated Ti6Al4V scaffold can significantly enhance the blood vessel formation. This study enlarges the application scope of magnesium and provides an optional choice to the conventional porous Ti6Al4V scaffold with enhanced osteogenesis and angiogenesis for further orthopedic applications.
DOI10.1016/j.bioactmat.2020.04.019
WOS IDWOS:000546576100023
Citation statistics
Cited Times:18[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.imr.ac.cn/handle/321006/155678
Collection中国科学院金属研究所
Recommended Citation
GB/T 7714
Gao, Peng,Fan, Bo,Yu, Xiaoming,et al. Biofunctional magnesium coated Ti6Al4V scaffold enhances osteogenesis and angiogenesis in vitro and in vivo for orthopedic application[J]. BIOACTIVE MATERIALS,2020,5(3):680-693.
APA Gao, Peng.,Fan, Bo.,Yu, Xiaoming.,Liu, Wenwen.,Wu, Jie.,...&Guo, Zheng.(2020).Biofunctional magnesium coated Ti6Al4V scaffold enhances osteogenesis and angiogenesis in vitro and in vivo for orthopedic application.BIOACTIVE MATERIALS,5(3),680-693.
MLA Gao, Peng,et al."Biofunctional magnesium coated Ti6Al4V scaffold enhances osteogenesis and angiogenesis in vitro and in vivo for orthopedic application".BIOACTIVE MATERIALS 5.3(2020):680-693.
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