In vitro bioactivity of AH plus with the addition of nano-magnesium hydroxide

doi:10.21037/atm.2020.02.133

IMR OpenIR

	In vitro bioactivity of AH plus with the addition of nano-magnesium hydroxide
	Sun, Xiujuan; Sun, Aoteng; Jia, Xingya; Jin, Shi; Zhang, Dan; Xiao, Keshen; Wang, Qiang
	2020-03-01
发表期刊	ANNALS OF TRANSLATIONAL MEDICINE
卷号	8 期号:6
摘要	Background: AH Plus (AH) has been widely used as a root canal sealer in the endodontic field due to its superior physicochemical properties. However, clinical application of AH is limited due to its weak bioactivity.Methods: In this study, we have developed an AH cement containing nano-magnesium hydroxide (NMH) as an additive to enhance the bioactivity of AH. The NMH can neutralize pH and facilitate bone formation. The objective of this study was to evaluate the effects of NMH and modified AH on osteoblasts behavior in vitro. The CCK-8, alkaline phosphatase (ALP) staining, and real-time polymerase chain reaction (PCR) assays were used to assess the proliferation and differentiation of MC3T3-E1 cells, respectively. The adhesion and spreading of MC3T3-E1 cells were investigated in vitro by scanning electron microscopy (SEM). Meanwhile, the flow and magnesium ion release of the modified AH was also concerned.Results: In vitro cell assays further showed that the addition of NMH into AH cement, which was denoted as modified AH (especially AH+3%NMH), could effectively improve the proliferation and osteogenic differentiation of MC3T3-E1 cells.Conclusions: Taken all together, we believe that the modified AH samples (especially AH+3%NMH) have outstanding biocompatibility and osteogenic properties and may have great potential in endodontic field.
DOI	10.21037/atm.2020.02.133
WOS记录号	WOS:000522784900056
引用统计	被引频次：3[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.imr.ac.cn/handle/321006/155711
专题	中国科学院金属研究所
推荐引用方式 GB/T 7714	Sun, Xiujuan,Sun, Aoteng,Jia, Xingya,et al. In vitro bioactivity of AH plus with the addition of nano-magnesium hydroxide[J]. ANNALS OF TRANSLATIONAL MEDICINE,2020,8(6).
APA	Sun, Xiujuan.,Sun, Aoteng.,Jia, Xingya.,Jin, Shi.,Zhang, Dan.,...&Wang, Qiang.(2020).In vitro bioactivity of AH plus with the addition of nano-magnesium hydroxide.ANNALS OF TRANSLATIONAL MEDICINE,8(6).
MLA	Sun, Xiujuan,et al."In vitro bioactivity of AH plus with the addition of nano-magnesium hydroxide".ANNALS OF TRANSLATIONAL MEDICINE 8.6(2020).