基质金属蛋白酶及其组织抑制物在子痫前期患者胎盘中的基因表达

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	基质金属蛋白酶及其组织抑制物在子痫前期患者胎盘中的基因表达
其他题名	mRNA expression of MMPs and TIMPs in placenta of preeclampsia
	Qiao Chong 1; Wang Chanhui 2; Luan Nannan 1; Chen Haoyang 1; Lin Qide 3; Shang Tao 1
	2005
发表期刊	中国现代医学杂志
ISSN	1005-8982
卷号	15.0 期号:022 页码:3361-3365
摘要	目的研究子痫前期患者胎盘组织中基质金属蛋白酶（MMP-9、MMP-2）及其组织抑制物（TIMP-1、TIMP-2）基因mRNA表达，探讨其与子痫前期发病、进展的关系。方法采用半定量RT—PCR方法对30例正常晚期妊娠和50例子痫前期患者（包括24例轻度和26例重度）胎盘组织中MMP-2、MMP-9、TIMP-1和TIMP-2的mRNA水平进行检测。结果子痫前期组胎盘组织的MMP-9基因表达水平显著下降（P〈0．01），重度子痫前期组MMP-9基因表达水平尤低（0．07±0．05）。尽管随病情的加重MMP-2mRNA水平呈现逐渐下降趋势，但没有统计学差异（P〉0．05）。子痛前期组胎盘组织中的TIMP-1mRNA水平明显高于正常晚期妊娠者（P〈0．01），重度子痫前期组更高（1．49±0．21）。子痫前期组胎盘组织TIMP-2mRNA表达显著降低（1．14±0．36），P〈0．01），并有随病情加重逐渐下降的趋势。结论随子痫前期患者病情的加重，胎盘组织的促浸润基因（MMP-2和MMP-9）表达水平降低，抑侵润基因（TIMP-1）的表达水平升高。子痫前期患者浸润相关基因的表达水平与子痫前期病情密切相关。
其他摘要	Objective To investigate the mRNA expressions of matrix metalloproteinase（MMP）-2,-9 and tissue inhibitior of metalloproteinase （TIMP）-1,-2 in placental tissue of patients with preeclampsia and their relation to the pathogenesis and development of preeclampsia. Methods Semi-quantitative RT-PCR analysis was used to detect the MMP-2, MMP-9, TIMP-1, TIMP-2mRNA expression levels in placental tissue of 50 cases of patients with preeclampsia （ 24 cases of mild and 26 cases of severe preeclampsia） and 30 cases of normal term pregnant women. Results The MMP-9 gene expression level was significantly reduced in placental tissue of preeclampsia （P 〈0.01）, which was the lowest in those of severe preeclampsia（0.07±0.05）. With the development of preeclampsia, the MMP- 2 gene expression level appeared a descent tendency without significance（P〉0.05）. The expression of TIMP-1 mRNA were significantly higher in the placental tissue of severe preeclampsia patients than those of normal term （P 〈0.01）, which was the highest in those of severe preeelampsia （1.49±0.21）. The TIMP-2 gene expression level was significantly reduced in placental tissue of preeclampsia （1.14±0.36, P 〈0.01）, which appeared a descent tendency with the severity of preeclampsia. Conclusions The expression of invasion related gene （MMP-2 and MMP-9） mRNA reduced and invasion suppressor gene （TIMP-1） mRNA increased with the development of preeclampsia. The expression of invasion related gene mRNA correlated with the severity of preeclampsia.
关键词	蛋白酶胎盘子痫前期
收录类别	CSCD
语种	中文
CSCD记录号	CSCD:2167327
引用统计
文献类型	期刊论文
条目标识符	http://ir.imr.ac.cn/handle/321006/155936
专题	中国科学院金属研究所
作者单位	1.中国医科大学 2.中国科学院金属研究所 3.Renji Hospital Affiliated of Shanghai Second Medical University
推荐引用方式 GB/T 7714	Qiao Chong,Wang Chanhui,Luan Nannan,等. 基质金属蛋白酶及其组织抑制物在子痫前期患者胎盘中的基因表达[J]. 中国现代医学杂志,2005,15.0(022):3361-3365.
APA	Qiao Chong,Wang Chanhui,Luan Nannan,Chen Haoyang,Lin Qide,&Shang Tao.(2005).基质金属蛋白酶及其组织抑制物在子痫前期患者胎盘中的基因表达.中国现代医学杂志,15.0(022),3361-3365.
MLA	Qiao Chong,et al."基质金属蛋白酶及其组织抑制物在子痫前期患者胎盘中的基因表达".中国现代医学杂志 15.0.022(2005):3361-3365.