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Serum protein fingerprint of patients with pancreatic cancer by SELDI technology
Alternative TitleSerum protein fingerprint of patients with pancreatic cancer by SELDI technology
Ma Ning1; Ge Chunlin1; Luan Fengming1; Yao Dianbo2; Hu Chaojun2; Li Ning2; Liu Yongfeng1
2008
Source PublicationCHINESE JOURNAL OF CANCER RESEARCH
ISSN1000-9604
Volume20Issue:3Pages:171-176
AbstractObjective: To study the serum protein fingerprint of patients with pancreatic cancer and to screen for protein molecules closely related to pancreatic cancer during the onset and progression of the disease using surface-enhanced laser desorption and ionization time of fight mass spectrometry (SELDI-TOF-MS). Methods: Serum samples from 20 pancreatic cancers, 20 healthy volunteers and 18 patients with other pancreatic diseases. WCX magnetic beans and PBSII-C protein chips reader (Ciphergen Biosystems Ins.) were used. The protein fingerprint expression of all the Serum samples and the resulting profiles between cancer and normal were analyzed with Biomarker Wizard system. Results: A group of proteomic peaks were detected. Four differently expressed potential biomarkers were identified with the relative molecular weights of 5705 Da, 4935 Da, 5318 Da and 3243 Da. Among them, two proteins with m/z5705, 5318Da down-regulated, and two proteins with m/z 4935, 3243 Da were up-regulated in pancreatic cancers. Conclusion: SELDI technology can be used to screen significant proteins of differential expression in the serum of pancreatic cancer patients. These different proteins could be specific biomarkers of the patients with pancreatic cancer in the serum and have the potential value of further investigation.
Other AbstractObjective To study the serum protein fingerprint of patients with pancreatic cancer and to screen for protein molecules closely related to pancreatic cancer during the onset and progression of the disease using surface-enhanced laser desorption and ionization time of fight mass spectrometry (SELDI-TOF-MS).Methods Serum samples from 20 pancreatic cancers, 20 healthy volunteers and 18 patients with other pancreatic diseases. WCX magnetic beans and PBSII-C protein chips reader (Ciphergen Biosystems Ins.) were used. The protein fingerprint expression of all the Serum samples and the resulting profiles between cancer and normal were analyzed with Biomarker Wizard system.Results A group of proteomic peaks were detected. Four differently expressed potential biomarkers were identified with the relative molecular weights of 5705 Da, 4935 Da, 5318 Da and 3243 Da. Among them, two proteins with m/z5705, 5318Da down-regulated, and two proteins with m/z 4935, 3243 Da were up-regulated in pancreatic cancers.Conclusion SELDI technology can be used to screen significant proteins of differential expression in the serum of pancreatic cancer patients. These different proteins could be specific biomarkers of the patients with pancreatic cancer in the serum and have the potential value of further investigation.
KeywordPROSTATE-CANCER MASS-SPECTROMETRY PROTEOMIC PATTERNS DIAGNOSIS IDENTIFICATION CARCINOMA EXPRESSION MARKER pancreatic cancer proteomic SELDI biological markers
Indexed ByCSCD
Language英语
Funding Project[Peking Union Medical College Hospital, Chinese Academy of Medical Science and Peking Union Medical College, Beijing]
CSCD IDCSCD:3360136
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Document Type期刊论文
Identifierhttp://ir.imr.ac.cn/handle/321006/156015
Collection中国科学院金属研究所
Affiliation1.中国科学院金属研究所
2.中国医学科学院
3.Peking Union Med Coll, Beijing 100730, Peoples R China
Recommended Citation
GB/T 7714
Ma Ning,Ge Chunlin,Luan Fengming,et al. Serum protein fingerprint of patients with pancreatic cancer by SELDI technology[J]. CHINESE JOURNAL OF CANCER RESEARCH,2008,20(3):171-176.
APA Ma Ning.,Ge Chunlin.,Luan Fengming.,Yao Dianbo.,Hu Chaojun.,...&Liu Yongfeng.(2008).Serum protein fingerprint of patients with pancreatic cancer by SELDI technology.CHINESE JOURNAL OF CANCER RESEARCH,20(3),171-176.
MLA Ma Ning,et al."Serum protein fingerprint of patients with pancreatic cancer by SELDI technology".CHINESE JOURNAL OF CANCER RESEARCH 20.3(2008):171-176.
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