IMR OpenIR
Reversion of Multidrug-Resistance by Proteasome Inhibitor Bortezomib in K562/DNR Cell Line
Alternative TitleReversion of Multidrug-Resistance by Proteasome Inhibitor Bortezomib in K562/DNR Cell Line
Wang Huihan; Li Yingchun; Liao Aijun; Fu Beibei; Yang Wei; Liu Zhuogang; Wang Xiaobin
2011
Source PublicationCHINESE JOURNAL OF CANCER RESEARCH
ISSN1000-9604
Volume23Issue:1Pages:69-73
AbstractObjective: To observe the reversion of multi-drug resistance by proteasome inhibitor bortezomib in K562/DNR cell line and to analyze the possible mechanism of reversion of multidrug-resistance.
Other AbstractObjective: To observe the reversion of multi‐drug resistance by proteasome inhibitor bortezomib in K562/DNR cell line and to analyze the possible mechanism of reversion of multidrug‐resistance. Methods: MTT method was used to determine the drug resistance of K562/DNR cells and the cellular toxicity of bortezomib. K562/DNR cells were cultured for 12 hours, 24 hours and 36 hours with 100 μg/ml DNR only or plus 4μg/L bortezomib. The expressions of NF‐κB, IκB and P‐gp of K562/DNR were detected with Western blot method, the activity of NF‐κB was tested by ELISA method and the apoptosis rate was observed in each group respectively. Results: The IC50 of DNR on cells of K562/S and K562/DNR groups were 1.16 μg/ml and 50.43 μg/mL, respectively. The drug‐resistant fold was 43.47. The IC10 of PS‐341 on Cell strain K562/DNR was 4 μg/L. Therefore, 4μg/L was selected as the concentration for PS‐341 to reverse drug‐resistance in this study. DNR induced down‐regulation of IκB expression, up‐regulation of NF‐κB and P‐gp expression. After treatment with PS‐341, a proteasome inhibitor, the IκB degradation was inhibited, IκB expression increased, NF‐κB and P‐gp expression decreased in a time dependent manner. Compared to DNR group, the NF‐κB p65 activity of DNR+PS‐341 group was decreased. Compared to Corresponding DNR group, DNR induced apoptosis rate increases after addition of PS‐341 in a time dependent manner. Conclusion: Proteasome inhibitor bortezomib can convert the leukemia cell drug resistance. The mechanism may be that bortezomib decreases the degradation of IκB and the expression of NF‐κB and P‐gp, therefore induces the apoptosis of multi‐drug resistant cells
KeywordMULTIPLE-MYELOMA CELLS FACTOR-KAPPA-B CHEMOTHERAPEUTIC-AGENTS THERAPEUTIC TARGET DRUG-RESISTANCE P-GLYCOPROTEIN LEUKEMIA CANCER MALIGNANCIES SENSITIVITY Bortezomib NF-kappa B Multi-drug resistance mdr1 gene P-gp K562 cells
Indexed ByCSCD
Language英语
Funding Project[Educational Commission of Liaoning Province, China]
CSCD IDCSCD:4173900
Citation statistics
Document Type期刊论文
Identifierhttp://ir.imr.ac.cn/handle/321006/156096
Collection中国科学院金属研究所
Affiliation中国科学院金属研究所
Recommended Citation
GB/T 7714
Wang Huihan,Li Yingchun,Liao Aijun,et al. Reversion of Multidrug-Resistance by Proteasome Inhibitor Bortezomib in K562/DNR Cell Line[J]. CHINESE JOURNAL OF CANCER RESEARCH,2011,23(1):69-73.
APA Wang Huihan.,Li Yingchun.,Liao Aijun.,Fu Beibei.,Yang Wei.,...&Wang Xiaobin.(2011).Reversion of Multidrug-Resistance by Proteasome Inhibitor Bortezomib in K562/DNR Cell Line.CHINESE JOURNAL OF CANCER RESEARCH,23(1),69-73.
MLA Wang Huihan,et al."Reversion of Multidrug-Resistance by Proteasome Inhibitor Bortezomib in K562/DNR Cell Line".CHINESE JOURNAL OF CANCER RESEARCH 23.1(2011):69-73.
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Wang Huihan]'s Articles
[Li Yingchun]'s Articles
[Liao Aijun]'s Articles
Baidu academic
Similar articles in Baidu academic
[Wang Huihan]'s Articles
[Li Yingchun]'s Articles
[Liao Aijun]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Wang Huihan]'s Articles
[Li Yingchun]'s Articles
[Liao Aijun]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.