Peripheral nerve allograft prepared by an acellular, hypotonic tissue engineering method

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	Peripheral nerve allograft prepared by an acellular, hypotonic tissue engineering method
其他题名	Peripheral nerve allograft prepared by an acellular,hypotonic tissue engineering method
	Sun Huizhe; Dai Wenying; Wang Jie; Tong Xiaojie; Tian Wei; Xu Jun
	2010
发表期刊	NEURAL REGENERATION RESEARCH
ISSN	1673-5374
卷号	5 期号:8 页码:630-634
摘要	BACKGROUND: Tissue engineered acellular nerves are good autologous nerve substitutes. Acellular peripheral nerves prepared using a conventional chemical extraction method cause a great deal of damage to nerve structures, and the allograft affects the nerve regeneration following transplantation.
其他摘要	BACKGROUND:Tissue engineered acellular nerves are good autologous nerve substitutes. Acellular peripheral nerves prepared using a conventional chemical extraction method cause a great deal of damage to nerve structures, and the allograff affects the nerve regeneration following transplantation.OBJECTIVE:To prepare peripheral nerve grafts through an acellular tissue engineering method, and observe their histology, ultrastructure, protein components and histocompatibility.DESIGN, TIME AND SETTING:A randomized, controlled, in vivo nerve tissue engineering experiment was performed at the Department of Biochemistry and Molecular Biology, Shenyang Medical College, China, from September 2006 to June 2007.MATERIALS:Triton X-100, Pepstatin A, Aprotinin and Leupeptin were purchased from Sigma, USA; Tris (hydroxymethyl) aminomethane was purchased from Gibco, USA.METHODS:The bilateral sciatic nerves of Wistar rats were harvested, treated with 0.05 mol/L Tris-HCI buffer, followed by proteinase inhibitor and Triton X-100 to prepare acellular peripheral nerves. The nerves were implanted in the quadriceps femoris muscle of healthy Wistar rats.MAIN OUTCOME MEASURES:Tissue structure and ultrastructure of acellular peripheral nerves were observed by optical microscopy and scanning electron microscopy. Growth associated proteins were analyzed by sodium dodecyl sulfate polyacrylamide gel electrophoresis. Nerve allograft and the surrounding muscles were observed by hematoxylin-eosin staining.RESULTS:Acellular treatment eliminated Schwann cells, epineurium or perineurium cells, myelin sheaths and axons of nerve fibers in normal peripheral nerves, while the spatial structure, comprising basement membrane tubes of Schwann cells and the extracellular matrix of perineurium and nerve fascicles was maintained. Protein bands at the region of 30 kD were no longer visible, had slightly decreased at 43 kD and remained unchanged at 65 kD. Following implantation for 7 days, epineurium cells were absorbed. However, increased fibroblasts, decreased newly-generated capillaries and maturation of granulation tissue were observed.CONCLUSION:The acellular nerve allograft prepared through the use of a hypotonic, acellular method displays good histocompatibility, eliminates immune substances and retains growth associated proteins that induce the growth of the neural axis. In addition, this method provides an ideal scaffold to construct artificial nerves.
关键词	SCHWANN-CELLS REGENERATION GROWTH CONDUIT MATRIX AXONS nerve peripheral nerve tissue engineering extracellular matrix regeneration allografts rats
收录类别	CSCD
语种	英语
CSCD记录号	CSCD:3868738
引用统计
文献类型	期刊论文
条目标识符	http://ir.imr.ac.cn/handle/321006/156099
专题	中国科学院金属研究所
作者单位	中国科学院金属研究所
推荐引用方式 GB/T 7714	Sun Huizhe,Dai Wenying,Wang Jie,et al. Peripheral nerve allograft prepared by an acellular, hypotonic tissue engineering method[J]. NEURAL REGENERATION RESEARCH,2010,5(8):630-634.
APA	Sun Huizhe,Dai Wenying,Wang Jie,Tong Xiaojie,Tian Wei,&Xu Jun.(2010).Peripheral nerve allograft prepared by an acellular, hypotonic tissue engineering method.NEURAL REGENERATION RESEARCH,5(8),630-634.
MLA	Sun Huizhe,et al."Peripheral nerve allograft prepared by an acellular, hypotonic tissue engineering method".NEURAL REGENERATION RESEARCH 5.8(2010):630-634.