| microRNA-218 promotes gemcitabine sensitivity in human pancreatic cancer cells by regulating HMGB1 expression | |
| Alternative Title | microRNA-218 promotes gemcitabine sensitivity in human pancreatic cancer cells by regulating HMGB1 expression |
| Liu Zhe; Du Ruixia; Long Jin; Guo Kejian; Ge Chunlin; Bi Shulong; Xu Yuanhong | |
| 2015 | |
| Source Publication | CHINESE JOURNAL OF CANCER RESEARCH
 |
| ISSN | 1000-9604 |
| Volume | 27Issue:3Pages:267-278 |
| Abstract | Objective: The purpose of this study was to examine the effect of gemcitabine (GEM) on microRNA-218 (miR-218) expression in human pancreatic cancer cells. |
| Other Abstract | Objective The purpose of this study was to examine the effect of gemcitabine (GEM) on microRNA-218 (miR-218) expression in human pancreatic cancer cells. Methods Quantitative reverse transcription polymerase chain reaction (qRT-PCR) was performed to examine the differences in miR-218 expression between the GEM-sensitive BxPC-3 pancreatic cancer cells and GEM-resistant PANC-1 cells. The effect of GEM on the expression of miR-218 in PANC-1 cells was also investigated. PANC-1 cells were transfected either with HMGB1 siRNA to knock down the expression of HMGB1 or with the recombinant HMGB1 expression vector (pcDNA3.1- HMGB1 ) to overexpress HMGB1 . The effect of ectopic expression of HMGB1 on the apoptosis of miR-218-transfected and GEM-treated PANC-1 cells was examined by flow cytometric analysis. Results The miR-218 expression level was lower in GEM-resistant PANC-1 cells compared to GEM-sensitive BxPC-3 cells (P<0.05). The percentage of apoptotic PANC-1 cells was significantly increased in the miR-218 mimic + GEM group compared to the mimic ctrl + GEM group and the normal control group (P<0.01). The HMGB1 expression level was markedly decreased in PANC-1 cells transfected with HMGB1 siRNA but was significantly increased in PANC-1 cells transfected with the recombinant HMGB1 expression vector, pcDNA3.1- HMGB1 (P<0.01). The proportion of apoptotic PANC-1 cells was significantly lower in the miR-218 mimic + GEM + pcDNA3.1- HMGB1 group compared to the miR-218 mimic + GEM + HMGB1 siRNA group (P<0.01). Conclusions The expression level of miR-218 was downregulated in the GEM-resistant cell line. miR-218 promoted the sensitivity of PANC-1 cells to GEM, which was achieved mainly through regulating the expression of HMGB1 in PANC-1 cells. |
| Keyword | GROUP BOX 1 PLUS GEMCITABINE OVARIAN-CANCER ADENOCARCINOMA THERAPY RESISTANCE DOCETAXEL AUTOPHAGY TARGET CHEMOTHERAPY Pancreatic cancer microRNA-218 (miR-218) gemcitabine (GEM) apoptosis high mobility group box 1 (HMGB1) |
| Indexed By | CSCD |
| Language | 英语 |
| Funding Project | [Liaoning Provincial Department of Education Science Research Project] ; [Liaoning Province Science and Technology Plan Project] ; [Shenyang Municipal Science and Technology Project] |
| CSCD ID | CSCD:5467266 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.imr.ac.cn/handle/321006/156201 |
| Collection | 中国科学院金属研究所 |
| Affiliation | 中国科学院金属研究所 |
| Recommended Citation GB/T 7714 | Liu Zhe,Du Ruixia,Long Jin,et al. microRNA-218 promotes gemcitabine sensitivity in human pancreatic cancer cells by regulating HMGB1 expression[J]. CHINESE JOURNAL OF CANCER RESEARCH,2015,27(3):267-278. |
| APA | Liu Zhe.,Du Ruixia.,Long Jin.,Guo Kejian.,Ge Chunlin.,...&Xu Yuanhong.(2015).microRNA-218 promotes gemcitabine sensitivity in human pancreatic cancer cells by regulating HMGB1 expression.CHINESE JOURNAL OF CANCER RESEARCH,27(3),267-278. |
| MLA | Liu Zhe,et al."microRNA-218 promotes gemcitabine sensitivity in human pancreatic cancer cells by regulating HMGB1 expression".CHINESE JOURNAL OF CANCER RESEARCH 27.3(2015):267-278. |
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