microRNA-218 promotes gemcitabine sensitivity in human pancreatic cancer cells by regulating HMGB1 expression

IMR OpenIR

	microRNA-218 promotes gemcitabine sensitivity in human pancreatic cancer cells by regulating HMGB1 expression
其他题名	microRNA-218 promotes gemcitabine sensitivity in human pancreatic cancer cells by regulating HMGB1 expression
	Liu Zhe; Du Ruixia; Long Jin; Guo Kejian; Ge Chunlin; Bi Shulong; Xu Yuanhong
	2015
发表期刊	CHINESE JOURNAL OF CANCER RESEARCH
ISSN	1000-9604
卷号	27 期号:3 页码:267-278
摘要	Objective: The purpose of this study was to examine the effect of gemcitabine (GEM) on microRNA-218 (miR-218) expression in human pancreatic cancer cells.
其他摘要	Objective The purpose of this study was to examine the effect of gemcitabine (GEM) on microRNA-218 (miR-218) expression in human pancreatic cancer cells. Methods Quantitative reverse transcription polymerase chain reaction (qRT-PCR) was performed to examine the differences in miR-218 expression between the GEM-sensitive BxPC-3 pancreatic cancer cells and GEM-resistant PANC-1 cells. The effect of GEM on the expression of miR-218 in PANC-1 cells was also investigated. PANC-1 cells were transfected either with HMGB1 siRNA to knock down the expression of HMGB1 or with the recombinant HMGB1 expression vector (pcDNA3.1- HMGB1 ) to overexpress HMGB1 . The effect of ectopic expression of HMGB1 on the apoptosis of miR-218-transfected and GEM-treated PANC-1 cells was examined by flow cytometric analysis. Results The miR-218 expression level was lower in GEM-resistant PANC-1 cells compared to GEM-sensitive BxPC-3 cells (P<0.05). The percentage of apoptotic PANC-1 cells was significantly increased in the miR-218 mimic + GEM group compared to the mimic ctrl + GEM group and the normal control group (P<0.01). The HMGB1 expression level was markedly decreased in PANC-1 cells transfected with HMGB1 siRNA but was significantly increased in PANC-1 cells transfected with the recombinant HMGB1 expression vector, pcDNA3.1- HMGB1 (P<0.01). The proportion of apoptotic PANC-1 cells was significantly lower in the miR-218 mimic + GEM + pcDNA3.1- HMGB1 group compared to the miR-218 mimic + GEM + HMGB1 siRNA group (P<0.01). Conclusions The expression level of miR-218 was downregulated in the GEM-resistant cell line. miR-218 promoted the sensitivity of PANC-1 cells to GEM, which was achieved mainly through regulating the expression of HMGB1 in PANC-1 cells.
关键词	GROUP BOX 1 PLUS GEMCITABINE OVARIAN-CANCER ADENOCARCINOMA THERAPY RESISTANCE DOCETAXEL AUTOPHAGY TARGET CHEMOTHERAPY Pancreatic cancer microRNA-218 (miR-218) gemcitabine (GEM) apoptosis high mobility group box 1 (HMGB1)
收录类别	CSCD
语种	英语
资助项目	[Liaoning Provincial Department of Education Science Research Project] ; [Liaoning Province Science and Technology Plan Project] ; [Shenyang Municipal Science and Technology Project]
CSCD记录号	CSCD:5467266
引用统计
文献类型	期刊论文
条目标识符	http://ir.imr.ac.cn/handle/321006/156201
专题	中国科学院金属研究所
作者单位	中国科学院金属研究所
推荐引用方式 GB/T 7714	Liu Zhe,Du Ruixia,Long Jin,et al. microRNA-218 promotes gemcitabine sensitivity in human pancreatic cancer cells by regulating HMGB1 expression[J]. CHINESE JOURNAL OF CANCER RESEARCH,2015,27(3):267-278.
APA	Liu Zhe.,Du Ruixia.,Long Jin.,Guo Kejian.,Ge Chunlin.,...&Xu Yuanhong.(2015).microRNA-218 promotes gemcitabine sensitivity in human pancreatic cancer cells by regulating HMGB1 expression.CHINESE JOURNAL OF CANCER RESEARCH,27(3),267-278.
MLA	Liu Zhe,et al."microRNA-218 promotes gemcitabine sensitivity in human pancreatic cancer cells by regulating HMGB1 expression".CHINESE JOURNAL OF CANCER RESEARCH 27.3(2015):267-278.