Effect of activin A on OVCAR-3 Ovarian epithelial cancer cells

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	Effect of activin A on OVCAR-3 Ovarian epithelial cancer cells
其他题名	Effect of activin A on OVCAR-3 Ovarian epithelial cancer cells
	Li Xiuqin; Ren Bo; Du Zhenhua
	2008
发表期刊	CHINESE JOURNAL OF CANCER RESEARCH
ISSN	1000-9604
卷号	20 期号:3 页码:186-189
摘要	Objective: To investigate the proliferation effect and its pathway of activin A on Ovarian epithelial cancer cells line OVCAR-3. Methods: OVCAR-3 cells were cultured in vitro and the membrane receptor ActR II was detected by immunohistochemical method. The OVCAR-3 cells were cultured with 10 ng/ml activin A for 7 d to observe the effects. Activin A at 5, 10, 15 and 20 ng/ml was used separately to treat the OVCAR-3 cancer cells for 24, 48 and 72 h in order to draw the growth proliferation rate curve measured by MTT method. The expression of protein bcl-2 was detected by western-blot. When OVCAR-3 cells were treated with 10 ng/ml activin A and 5 mu g/ml DDP for 24, 48 and 72 h, cell apoptosis could be detected by electron microscopy and flow cytometry (FCM). Results: Positive expression of ActR II was detected. We also found that the proliferation of OVCAR-3 reached to the climax on the 5th day of culture. The experiments showed that the cells treated with activin A increased quickly and grew faster than those in control group. Moreover, OVCAR-3 cells treated with activin A for 48 h proliferated significantly greater than those treated for 24 h or 72 h (P < 0.01). bcl-2 protein expression increased expression in activin A treated group than in control group (P < 0.05). Conclusion: Activin A could increase the proliferation of OVCAR-3 cells which may be through bcl-2 anti-apoptosis pathway.
其他摘要	Objective To investigate the proliferation effect and its pathway of activin A on Ovarian epithelial cancer cells line OVCAR-3. Methods OVCAR-3 cells were cultured in vitro and the membrane receptor ActR II was detected by immunohistochemical method. The OVCAR-3 cells were cultured with 10 ng/ml activin A for 7 d to observe the effects. Activin A at 5, 10, 15 and 20 ng/ml was used separately to treat the OVCAR-3 cancer cells for 24, 48 and 72 h in order to draw the growth proliferation rate curve measured by MTT method. The expression of protein bcl-2 was detected by western-blot. When OVCAR-3 cells were treated with 10 ng/ml activin A and 5 μg/ml DDP for 24, 48 and 72 h, cell apoptosis could be detected by electron microscopy and flow cytometry (FCM).Results Positive expression of ActR II was detected. We also found that the proliferation of OVCAR-3 reached to the climax on the 5th day of culture. The experiments showed that the cells treated with activin A increased quickly and grew faster than those in control group. Moreover, OVCAR-3 cells treated with activin A for 48 h proliferated significantly greater than those treated for 24 h or 72 h (P<0.01). bcl-2 protein expression increased expression in activin A treated group than in control group (P<0.05). Conclusion Activin A could increase the proliferation of OVCAR-3 cells which may be through bcl-2 anti-apoptosis pathway.
关键词	SURFACE EPITHELIUM GROWTH-FACTOR BCL-2 FAMILY EXPRESSION APOPTOSIS INHIBIN Ovarian cancer OVCAR-3 cells line activin A bcl-2 protein MTT
收录类别	CSCD
语种	英语
CSCD记录号	CSCD:3360139
引用统计
文献类型	期刊论文
条目标识符	http://ir.imr.ac.cn/handle/321006/156350
专题	中国科学院金属研究所
作者单位	中国科学院金属研究所
推荐引用方式 GB/T 7714	Li Xiuqin,Ren Bo,Du Zhenhua. Effect of activin A on OVCAR-3 Ovarian epithelial cancer cells[J]. CHINESE JOURNAL OF CANCER RESEARCH,2008,20(3):186-189.
APA	Li Xiuqin,Ren Bo,&Du Zhenhua.(2008).Effect of activin A on OVCAR-3 Ovarian epithelial cancer cells.CHINESE JOURNAL OF CANCER RESEARCH,20(3),186-189.
MLA	Li Xiuqin,et al."Effect of activin A on OVCAR-3 Ovarian epithelial cancer cells".CHINESE JOURNAL OF CANCER RESEARCH 20.3(2008):186-189.