Isolation of hemoglobin with metal-organic frameworks Y(BTC)(H2O)(6)

IMR OpenIR

	Isolation of hemoglobin with metal-organic frameworks Y(BTC)(H2O)(6)
其他题名	Isolation of hemoglobin with metal–organic frameworks Y(BTC)(H2O)6
	Shu Yang 1; Meng Ying 1; Chen MingLi 1; Wang JianHua 1
	2015
发表期刊	CHINESE CHEMICAL LETTERS
ISSN	1001-8417
卷号	26 期号:12 页码:1460-1464
摘要	The hierarchical metal-organic frameworks (MOFs), such as Y(BTC)(H2O)(6), are prepared with yttrium nitrate and benzene-1,3,5-tricarboxylic acid at room temperature. The product is characterized by Fourier transform infrared (FT-IR), X-ray diffraction (XRD), scanning electron microscopy (SEM) and thermogravimetric analysis (TGA). The Y(BTC)(H2O)(6) particles are sufficiently rigid for performing solid phase extraction and they exhibit favorable selectivity toward the adsorption of hemoglobin. The adsorption behavior of hemoglobin onto the Y(BTC)(H2O)(6) fits the Langmuir adsorption model with a theoretical adsorption capacity of 555.6 mg g(-1). An adsorption efficiency of 87.7% for 100 mu g mL(-1) hemoglobin in 1 mL sample solution (at pH 6.0) is achieved with 0.40 mg Y(BTC)(H2O)(6). 77.3% of the retained hemoglobin is readily recovered using a 0.5% (m/v) SDS solution as the stripping reagent. Circular dichroism spectra indicated that the conformation of hemoglobin is maintained during the adsorption-desorption process. The MOFs material is applied for the isolation of hemoglobin from human blood and the purity of the obtained hemoglobin is further verified by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). (C) 2015 Chinese Chemical Society and Institute of Materia Medica, Chinese Academy of Medical Sciences. Published by Elsevier B.V. All rights reserved.
其他摘要	The hierarchical metal–organic frameworks (MOFs), such as Y(BTC)(H_2O)_6, are prepared with yttrium nitrate and benzene-1,3,5-tricarboxylic acid at room temperature. The product is characterized by Fourier transform infrared (FT-IR), X-ray diffraction (XRD), scanning electron microscopy (SEM) and thermogravimetric analysis (TGA). The Y(BTC)(H_2O)_6 particles are sufficiently rigid for performing solid phase extraction and they exhibit favorable selectivity toward the adsorption of hemoglobin. The adsorption behavior of hemoglobin onto the Y(BTC)(H_2O)_6 fits the Langmuir adsorption model with a theoretical adsorption capacity of 555.6 mg g~(-1). An adsorption efficiency of 87.7% for 100 mgmL~(-1) hemoglobin in 1 mL sample solution (at pH 6.0) is achieved with 0.40 mg Y(BTC)(H_2O)_6. 77.3% of the retained hemoglobin is readily recovered using a 0.5% (m/v) SDS solution as the stripping reagent. Circular dichroism spectra indicated that the conformation of hemoglobin is maintained during the adsorption–desorption process. The MOFs material is applied for the isolation of hemoglobin from human blood and the purity of the obtained hemoglobin is further verified by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS–PAGE).
关键词	SELECTIVE ISOLATION COMPOSITES ENRICHMENT ADSORPTION PROTEINS Metal-organic frameworks Hemoglobin Solid-phase extraction Protein solation
收录类别	CSCD
语种	英语
资助项目	[National Natural Science Foundation of China] ; [Fundamental Research Funds for the Central Universities]
CSCD记录号	CSCD:5605583
引用统计	被引频次：1[CSCD] [CSCD记录]
文献类型	期刊论文
条目标识符	http://ir.imr.ac.cn/handle/321006/156556
专题	中国科学院金属研究所
作者单位	1.东北大学 2.中国科学院金属研究所
推荐引用方式 GB/T 7714	Shu Yang,Meng Ying,Chen MingLi,et al. Isolation of hemoglobin with metal-organic frameworks Y(BTC)(H2O)(6)[J]. CHINESE CHEMICAL LETTERS,2015,26(12):1460-1464.
APA	Shu Yang,Meng Ying,Chen MingLi,&Wang JianHua.(2015).Isolation of hemoglobin with metal-organic frameworks Y(BTC)(H2O)(6).CHINESE CHEMICAL LETTERS,26(12),1460-1464.
MLA	Shu Yang,et al."Isolation of hemoglobin with metal-organic frameworks Y(BTC)(H2O)(6)".CHINESE CHEMICAL LETTERS 26.12(2015):1460-1464.