Fabrication and Characterization of Nanopillar-Like HA Coating on Porous Ti6Al4V Scaffold by a Combination of Alkali–Acid-Heat and Hydrothermal Treatments

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	Fabrication and Characterization of Nanopillar-Like HA Coating on Porous Ti6Al4V Scaffold by a Combination of Alkali–Acid-Heat and Hydrothermal Treatments
其他题名	Fabrication and Characterization of Nanopillar-Like HA Coating on Porous Ti6Al4V Scaffold by a Combination of Alkali–Acid-Heat and Hydrothermal Treatments
	Li Junlei 1; Wang Shuai 1; Cao Fang 1; Lin Xiao 2; Wei Xiaowei 1; Zhao Zhenhua 1; Dou Xiaojie 1; Yu Weiting 1; Yang Ke 3; Zhao Dewei 1
	2019
发表期刊	Acta Metallurgica Sinica(English Letters)
ISSN	1006-7191
卷号	32 期号:9 页码:1075-1088
摘要	Porous titanium scaffold with suitable porous architecture exhibits enormous potentials for bone defect repairs. However, insufficient osteointegration and osteoinduction still remain to open as one of the major problems to achieve satisfactory therapeutic effect. To solve this problem, many studies have been carried out to improve the bioactivity of porous titanium scaffold by surface modifications. In this study, porous Ti6Al4V scaffolds were fabricated using additive manufacturing technique. Porous architectures were built up based on a diamond pore structure unit. Alkali–acid-heat (AH) treatment was applied to create a TiO_2 layer on the porous Ti6Al4V scaffold (AH-porous Ti6Al4V). Subsequently, a hydrothermal treatment was employed to enable the formation of HA coating with nanopillar-like morphology on the alkali–acid-heat-treated surface (HT/AH-porous Ti6Al4V). The effects of surface modifications on apatite-forming ability, protein adsorption, cell attachment, cell proliferation and osteogenic gene expression were studied using apatite-forming ability test, protein adsorption assay and in vitro cell culture assay. It was found that the HT/AH-porous Ti6Al4V exhibited the highest apatite formation ability and best affinity to fibronectin and vitronectin. In vitro studies indicated that the mesenchymal stem cells (MSCs) cultured on the HT/AH-porous Ti6Al4V presented improved adhesion and differentiation compared with the porous Ti6Al4V and AH-porous Ti6Al4V.
其他摘要	Porous titanium scaffold with suitable porous architecture exhibits enormous potentials for bone defect repairs. However, insufficient osteointegration and osteoinduction still remain to open as one of the major problems to achieve satisfactory therapeutic effect. To solve this problem, many studies have been carried out to improve the bioactivity of porous titanium scaffold by surface modifications. In this study, porous Ti6Al4V scaffolds were fabricated using additive manufacturing technique. Porous architectures were built up based on a diamond pore structure unit. Alkali–acid-heat (AH) treatment was applied to create a TiO_2 layer on the porous Ti6Al4V scaffold (AH-porous Ti6Al4V). Subsequently, a hydrothermal treatment was employed to enable the formation of HA coating with nanopillar-like morphology on the alkali–acid-heat-treated surface (HT/AH-porous Ti6Al4V). The effects of surface modifications on apatite-forming ability, protein adsorption, cell attachment, cell proliferation and osteogenic gene expression were studied using apatite-forming ability test, protein adsorption assay and in vitro cell culture assay. It was found that the HT/AH-porous Ti6Al4V exhibited the highest apatite formation ability and best affinity to fibronectin and vitronectin. In vitro studies indicated that the mesenchymal stem cells (MSCs) cultured on the HT/AH-porous Ti6Al4V presented improved adhesion and differentiation compared with the porous Ti6Al4V and AH-porous Ti6Al4V.
关键词	Additive manufacturing Biomaterials Porous titanium scaffold HA coating
收录类别	CSCD
语种	英语
CSCD记录号	CSCD:6563712
引用统计	被引频次：5[CSCD] [CSCD记录]
文献类型	期刊论文
条目标识符	http://ir.imr.ac.cn/handle/321006/156684
专题	中国科学院金属研究所
作者单位	1.大连大学 2.苏州大学 3.中国科学院金属研究所
推荐引用方式 GB/T 7714	Li Junlei,Wang Shuai,Cao Fang,等. Fabrication and Characterization of Nanopillar-Like HA Coating on Porous Ti6Al4V Scaffold by a Combination of Alkali–Acid-Heat and Hydrothermal Treatments[J]. Acta Metallurgica Sinica(English Letters),2019,32(9):1075-1088.
APA	Li Junlei.,Wang Shuai.,Cao Fang.,Lin Xiao.,Wei Xiaowei.,...&Zhao Dewei.(2019).Fabrication and Characterization of Nanopillar-Like HA Coating on Porous Ti6Al4V Scaffold by a Combination of Alkali–Acid-Heat and Hydrothermal Treatments.Acta Metallurgica Sinica(English Letters),32(9),1075-1088.
MLA	Li Junlei,et al."Fabrication and Characterization of Nanopillar-Like HA Coating on Porous Ti6Al4V Scaffold by a Combination of Alkali–Acid-Heat and Hydrothermal Treatments".Acta Metallurgica Sinica(English Letters) 32.9(2019):1075-1088.