| Neural cell injury microenvironment induces neural differentiation of human umbilical cord mesenchymal stem cells | |
| Alternative Title | Neural cell injury microenvironment induces neural differentiation of human umbilical cord mesenchymal stem cells ☆ |
| Zhou Jin; Tian Guoping; Wang Jinge; Luo Xiaoguang; Zhang Siyang; Li Jianping; Li Li; Xu Bing; Zhu Feng; Wang Xia; Jia Chunhong; Zhao Weijin; Zhao Danyang; Xu Aihua | |
| 2012 | |
| Source Publication | NEURAL REGENERATION RESEARCH
 |
| ISSN | 1673-5374 |
| Volume | 7Issue:34Pages:2689-2697 |
| Abstract | This study aimed to investigate the neural differentiation of human umbilical cord mesenchymal stem cells (hUCMSCs) under the induction of injured neural cells. After in vitro isolation and culture, passage 5 hUCMSCs were used for experimentation. hUCMSCs were co-cultured with normal or A beta(1-40)-injured PC12 cells, PC12 cell supernatant or PC12 cell lysate in a Transwell co-culture system. Western blot analysis and flow cytometry results showed that choline acetyltransferase and microtubule-associated protein 2, a specific marker for neural cells, were expressed in hUCMSCs under various culture conditions, and highest expression was observed in the hUCMSCs co-cultured with injured PC12 cells. Choline acetyltransferase and microtubule-associated protein 2 were not expressed in hUCMSCs cultured alone (no treatment). Cell Counting Kit-8 assay results showed that hUCMSCs under co-culture conditions promoted the proliferation of injured PC12 cells. These findings suggest that the microenvironment during neural tissue injury can effectively induce neural cell differentiation of hUCMSCs. These differentiated hUCMSCs likely accelerate the repair of injured neural cells. |
| Other Abstract | This study aimed to investigate the neural differentiation of human umbilical cord mesenchymal stem cells (hUCMSCs) under the induction of injured neural cells. After in vitro isolation and culture, passage 5 hUCMSCs were used for experimentation. hUCMSCs were co-cultured with normal or Aβ 1-40 -injured PC12 cells, PC12 cell supernatant or PC12 cell lysate in a Transwell co-culture system. Western blot analysis and flow cytometry results showed that choline acetyltransferase and microtubule-associated protein 2, a specific marker for neural cells, were expressed in hUCMSCs under various culture conditions, and highest expression was observed in the hUCMSCs co-cultured with injured PC12 cells. Choline acetyltransferase and microtubule-associated protein 2 were not expressed in hUCMSCs cultured alone (no treatment). Cell Counting Kit-8 assay results showed that hUCMSCs under co-culture conditions promoted the proliferation of injured PC12 cells. These findings suggest that the microenvironment during neural tissue injury can effectively induce neural cell differentiation of hUCMSCs. These differentiated hUCMSCs likely accelerate the repair of injured neural cells. |
| Keyword | BETA-AMYLOID NEUROTOXICITY ALZHEIMERS-DISEASE CHOLINE-ACETYLTRANSFERASE RAT MODEL IN-VITRO REGENERATIVE MEDICINE HIPPOCAMPAL-NEURONS COGNITIVE FUNCTION SCHWANN-CELLS BONE-MARROW stem cell umbilical cord mesenchymal stem cell co-culture induction differentiation neural cell microtubule-associated protein 2 injured cell Transwell neural regeneration regeneration |
| Indexed By | CSCD |
| Language | 英语 |
| CSCD ID | CSCD:4747648 |
| Citation statistics |
Cited Times:2[CSCD]
[CSCD Record]
|
| Document Type | 期刊论文 |
| Identifier | http://ir.imr.ac.cn/handle/321006/156952 |
| Collection | 中国科学院金属研究所 |
| Affiliation | 中国科学院金属研究所 |
| Recommended Citation GB/T 7714 | Zhou Jin,Tian Guoping,Wang Jinge,et al. Neural cell injury microenvironment induces neural differentiation of human umbilical cord mesenchymal stem cells[J]. NEURAL REGENERATION RESEARCH,2012,7(34):2689-2697. |
| APA | Zhou Jin.,Tian Guoping.,Wang Jinge.,Luo Xiaoguang.,Zhang Siyang.,...&Xu Aihua.(2012).Neural cell injury microenvironment induces neural differentiation of human umbilical cord mesenchymal stem cells.NEURAL REGENERATION RESEARCH,7(34),2689-2697. |
| MLA | Zhou Jin,et al."Neural cell injury microenvironment induces neural differentiation of human umbilical cord mesenchymal stem cells".NEURAL REGENERATION RESEARCH 7.34(2012):2689-2697. |
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