| Expression and function of Delta-like ligand 4 in a rat model of retinopathy of prematurity | |
| Alternative Title | Expression and function of Delta-like ligand 4 in a rat model of retinopathy of prematurity ☆ |
| Shi Shaoyang1; Li Xun1; Li You1; Pei Cunwen1; Yang Hongwei1; Chen Xiaolong1 | |
| 2013 | |
| Source Publication | NEURAL REGENERATION RESEARCH
 |
| ISSN | 1673-5374 |
| Volume | 8Issue:8Pages:723-730 |
| Abstract | The Delta-like ligand 4/Notch signaling pathway was shown to participate in the process of retinal development and angiogenesis. However, the function of the Delta-like ligand 4/Notch signaling pathway in retinopathy of prematurity requires further study. Retinopathy of prematurity was induced in 5-day-old Sprague-Dawley rats exposed to hyperoxia for 7 days, and then returned to room air. Reverse transcription-PCR and western blot revealed that Delta-like ligand 4 levels decreased at postnatal day 12 and increased at postnatal day 17 in retinopathy of prematurity rats. Flat-mounted adenosine diphosphatase stained retina and hematoxylin-eosin stained retinal tissue slices showed that the clock hour scores and the nuclei counts in retinopathy of prematurity rats were significantly different compared to normal control rats. After retinopathy of prematurity rats were intravitreally injected with Delta-like ligand 4 monoclonal antibody to inhibit the Delta-like ligand 4/Notch signaling pathway, there was a significant increase in the severity of retinal neovascularization (clock hours) in the intravitreally injected eyes. The nuclei count was highly correlated with the clock hour score. These results suggest that Delta-like ligand 4/Notch signaling plays an essential role in the process of physiological and pathological angiogenesis in the retina. |
| Other Abstract | The Delta-like ligand 4 |
| Keyword | OXYGEN-INDUCED RETINOPATHY ENDOTHELIAL GROWTH-FACTOR TIP CELL-FORMATION TUMOR ANGIOGENESIS RETINAL NEOVASCULARIZATION THERAPEUTIC IMPLICATIONS NOTCH VEGF INHIBITION PREVENTION neural regeneration peripheral nerve injury Delta-like ligand 4 retinopathy of prematurity retinal neovascularization vascular endothelial cells vascular endothelial growth factor Notch signaling pathway oxygen-induced retinopathy optic nerve disease photographs-containing paper neuroregeneration |
| Indexed By | CSCD |
| Language | 英语 |
| CSCD ID | CSCD:4786839 |
| Citation statistics |
Cited Times:1[CSCD]
[CSCD Record]
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| Document Type | 期刊论文 |
| Identifier | http://ir.imr.ac.cn/handle/321006/157501 |
| Collection | 中国科学院金属研究所 |
| Affiliation | 1.中国科学院金属研究所 2.230 Hosp Chinese PLA, Department Ophthalmol, Dandong 118000, Liaoning Provin, Peoples R China Department Ophthalmol |
| Recommended Citation GB/T 7714 | Shi Shaoyang,Li Xun,Li You,et al. Expression and function of Delta-like ligand 4 in a rat model of retinopathy of prematurity[J]. NEURAL REGENERATION RESEARCH,2013,8(8):723-730. |
| APA | Shi Shaoyang,Li Xun,Li You,Pei Cunwen,Yang Hongwei,&Chen Xiaolong.(2013).Expression and function of Delta-like ligand 4 in a rat model of retinopathy of prematurity.NEURAL REGENERATION RESEARCH,8(8),723-730. |
| MLA | Shi Shaoyang,et al."Expression and function of Delta-like ligand 4 in a rat model of retinopathy of prematurity".NEURAL REGENERATION RESEARCH 8.8(2013):723-730. |
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