Interleukin-2 expression and glioma cell proliferation following Vaccinia vector gene transfection in vivo

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	Interleukin-2 expression and glioma cell proliferation following Vaccinia vector gene transfection in vivo
其他题名	Interleukin-2 expression and glioma cell proliferation following Vaccinia vector gene transfection in vivo
	Wang Xiaogang; Wei Xuezhong; Liu Jiangqiu
	2008
发表期刊	NEURAL REGENERATION RESEARCH
ISSN	1673-5374
卷号	3 期号:4 页码:415-417
摘要	BACKGROUND: The effectiveness of gene therapy is closely related to the efficiency of vector transfection and expression.
其他摘要	BACKGROUND: The effectiveness of gene therapy is closely related to the efficiency of vector transfection and expression.OBJECTIVE: This study was designed to transfect a human brain glioma cell line with recombinant Vaccinia virus expressing the interleukin-2 (rVV-IL-2) gene, and to observe IL-2 expression and glioma cell proliferation potential after transfection.DESIGN: Experimental observation.SETTING: Department of Neurosurgery, Shenyang Military Area Command of Chinese PLA.MATERIALS: The rVV-IL-2 vectors were obtained through homologous recombination and screening in the Second Military Medical University of Chinese PLA. The human brain glioma cell line and IL-2-dependent cells were produced by the Second Military Medical University of Chinese PLA. Human IL-2 was produced by Genzyme Corporation.METHODS: At passage day 1, Vero cells were amplified 1∶1 for virus and cells. A human brain glioma cell line was transfected using amplified Vaccinia viral vectors at varying multiplicities of infection (MOI). At 2, 4, 6, 8, 12, and 24 hours post-transfection, supernatant was collected to determine by MTT assay IL-2 expression levels in IL-2 dependent cells. The transfected and non-transfected cells were divided into 4 groups, namely MOI 1∶1, MOI 5∶1, MOI 10∶1, and control groups.MAIN OUTCOME MEASURES: IL-2 expression at different time points after transfection of human brain glioma cells with varying MOI of Vaccinia viral vectors; in vitro proliferation capacity of human brain glioma cells among the 4 groups.RESULTS: IL-2 expression was detectable 4 hours after Vaccinia viral vector transfection and reached 300 kU/L by 8 hours. There was no significant difference in the proliferating rate of human brain glioma cells among the 4 groups (P > 0.05).CONCLUSION: Vaccinia viral vectors can transfect human brain glioma cells in vitro and express high levels of IL-2.Vaccinia virus and high IL-2 expression do not influence the proliferation rate of human brain glioma cells in vitro.
关键词	MURINE MODEL IL-2 THERAPY RESPONSES ANTIGEN CANCER IMMUNITY HEAD Vaccinia virus vector glioma cell proliferation potential in vitro interleukin-2
收录类别	CSCD
语种	英语
CSCD记录号	CSCD:3299687
引用统计
文献类型	期刊论文
条目标识符	http://ir.imr.ac.cn/handle/321006/157715
专题	中国科学院金属研究所
作者单位	中国科学院金属研究所
推荐引用方式 GB/T 7714	Wang Xiaogang,Wei Xuezhong,Liu Jiangqiu. Interleukin-2 expression and glioma cell proliferation following Vaccinia vector gene transfection in vivo[J]. NEURAL REGENERATION RESEARCH,2008,3(4):415-417.
APA	Wang Xiaogang,Wei Xuezhong,&Liu Jiangqiu.(2008).Interleukin-2 expression and glioma cell proliferation following Vaccinia vector gene transfection in vivo.NEURAL REGENERATION RESEARCH,3(4),415-417.
MLA	Wang Xiaogang,et al."Interleukin-2 expression and glioma cell proliferation following Vaccinia vector gene transfection in vivo".NEURAL REGENERATION RESEARCH 3.4(2008):415-417.