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Effects of cardiopulmonary bypass on tight junction protein expressions in intestinal mucosa of rats
Alternative TitleEffects of cardiopulmonary bypass on tight junction protein expressions in intestinal mucosa of rats
Sun YingJie; Chen WeiMin; Zhang TieZheng; Cao HuiJuan; Zhou Jin
2008
Source PublicationWORLD JOURNAL OF GASTROENTEROLOGY
ISSN1007-9327
Volume14Issue:38Pages:5868-5875
AbstractAIM: To investigate the tight junction protein expressions of intestinal mucosa in an experimental model of cardiopulmonary bypass (CPB) in rats.
Other AbstractAIM: To investigate the tight junction protein expressions of intestinal mucosa in an experimental model of cardiopulmonary bypass (CPB) in rats. METHODS: Thirty anesthetized rats were randomly divided into two groups: Group S (n=10) served as sham operation and group C (n = 20) served as CPB which underwent CPB for 1 h. Expression of occludin and zonula occludens-1 (ZO-1) were determined by Western blotting and immunotochemistry, respectively. Plasma levels of diamine oxidase (DAO) and d-lactate were determined using an enzymatic spectrophotometry.RESULTS: Immunohistochemical localization of occludin and ZO-1 showed disruption of the tight junctions in enterocytes lining villi at the end of CPB and 2 h after CPB. The intensities of the occludin and ZO-1 at the end of CPB were lower than those of control group (76.4%±22.5% vs 96.5%±28.5% and 62.4%±10.1% vs 85.5%±25.6%, P <0.05) and were further lower at 2 h after CPB (50.5% ±10.5% and 45.3%±9.5%, P <0.05). Plasma d-lactate and DAO levels increased significantly (8.688 ±0.704 vs 5.745±0.364 and 0.898±0.062 vs 0.562±0.035, P<0.05) at the end of CPB compared with control group and were significantly higher at 2 h after CPB than those at the end of CPB (9.377 ±0.769 and 1.038±0.252, P <0.05). There were significant negative correlations between occludin or ZO-1 expression and DAO (r2= 0.5629,r2= 0.5424, P <0.05) or d-lactate levels (r2 = 0.6512,r2 = 0.7073, P <0.05) both at the end of CPB and 2 h after CPB.CONCLUSION: CPB markedly down-regulates the expression of occludin and ZO-1 proteins in intestinal mucosa of rats. The close correlation between expression of tight junctions (TJs) and plasma levels of DAO or d-lactate supports the hypothesis that intestinal permeability increases during and after CPB because of decreases in the expressions of TJs.
KeywordINDUCED INFLAMMATORY RESPONSE MULTIPLE ORGAN FAILURE BLOOD LACTATE LEVELS HUMAN SEPTIC SHOCK NITRIC-OXIDE BACTERIAL TRANSLOCATION EPITHELIAL BARRIER DIAMINE OXIDASE CARDIAC-SURGERY PERMEABILITY Cardiopulmonary bypass Occludin ZO-1 Tight junction Diamine oxidase d-lactate
Indexed ByCSCD
Language英语
CSCD IDCSCD:3431832
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Document Type期刊论文
Identifierhttp://ir.imr.ac.cn/handle/321006/157747
Collection中国科学院金属研究所
Affiliation中国科学院金属研究所
Recommended Citation
GB/T 7714
Sun YingJie,Chen WeiMin,Zhang TieZheng,et al. Effects of cardiopulmonary bypass on tight junction protein expressions in intestinal mucosa of rats[J]. WORLD JOURNAL OF GASTROENTEROLOGY,2008,14(38):5868-5875.
APA Sun YingJie,Chen WeiMin,Zhang TieZheng,Cao HuiJuan,&Zhou Jin.(2008).Effects of cardiopulmonary bypass on tight junction protein expressions in intestinal mucosa of rats.WORLD JOURNAL OF GASTROENTEROLOGY,14(38),5868-5875.
MLA Sun YingJie,et al."Effects of cardiopulmonary bypass on tight junction protein expressions in intestinal mucosa of rats".WORLD JOURNAL OF GASTROENTEROLOGY 14.38(2008):5868-5875.
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