Hypermethylation and aberrant expression of secreted fizzled-related protein genes in pancreatic cancer

IMR OpenIR

	Hypermethylation and aberrant expression of secreted fizzled-related protein genes in pancreatic cancer
其他题名	Hypermethylation and aberrant expression of secreted fizzled-related protein genes in pancreatic cancer
	Bu XianMin; Zhao ChengHai; Zhang Ning; Gao Feng; Lin Shuai; Dai XianWei
	2008
发表期刊	WORLD JOURNAL OF GASTROENTEROLOGY
ISSN	1007-9327
卷号	14 期号:21 页码:3421-3424
摘要	AIM: To determine the methylation status and aberrant expression of some secreted frizzled-related protein (SFRP) genes in pancreatic cancer and explore their role in pancreatic carcinogenesis.
其他摘要	AIM:To determine the methylation status and aberrant expression of some secreted frizzled-related protein (SFRP) genes in pancreatic cancer and explore their role in pancreatic carcinogenesis. METHODS:Methylation status and expression of SFRP genes were detected by methylation-specific PCR (MSPCR) and reverse-transcription PCR (RT-PCR) respectively. RESULTS:The frequencies of methylation for SFRP genes 1,2,4,5 were 70%, 48.3%,60% and 76.7% in pancreatic cancer samples, and 21.7%, 20%,10% and 36.7% in matched cancer adjacent normal tissue samples,respectively (χ2=28.23,P<0.0001 for SFRP gene 1; χ2=10.71,P=0.001 for SFRP gene 2;χ2=32.97,P<0.0001 for SFRP gene 4;χ2=19.55,P<0.0001 for SFRP gene 5). Expression loss of SFRP genes 1,2,4 and 5 was found in 65%,40%,55% and 71.7% of 60 pancreatic cancer samples, and 25%,15%,18.3% and 31.7% of matched cancer adjacent normal tissue samples,respectively (χ2=19.39,P<0.0001 for SFRP gene 1;χ2=9.40,P=0.002 for SFRP gene 2;χ2=17.37,P<0.0001 for SFRP gene 4;χ2=19.22,P<0.0001 for SFRP gene 5).SFRP gene 1 was methylated but not expressed in PC-3 and PANC-1,SFRP gene 2 was methylated but not expressed in PANC-1 and CFPAC-1,SFRP gene 4 was methylated but not expressed in PC-3,and SFRP gene 5 was methylated but not expressed in CFPAC-1. CONCLUSION:Hypermethylation and aberrant expression of SFRP genes are common in pancreatic cancer,which may be involved in pancreatic carcinogenesis.
关键词	WNT ANTAGONIST SFRP1 BETA-ESTRADIOL UP-REGULATION MCF-7 CELLS WNT/BETA-CATENIN PROSTATE-CANCER TUMOR ADENOCARCINOMA METHYLATION TUMORIGENESIS hypermethylation secreted frizzled-related protein pancreatic cancer
收录类别	CSCD
语种	英语
CSCD记录号	CSCD:3317612
引用统计
文献类型	期刊论文
条目标识符	http://ir.imr.ac.cn/handle/321006/157967
专题	中国科学院金属研究所
作者单位	中国科学院金属研究所
推荐引用方式 GB/T 7714	Bu XianMin,Zhao ChengHai,Zhang Ning,et al. Hypermethylation and aberrant expression of secreted fizzled-related protein genes in pancreatic cancer[J]. WORLD JOURNAL OF GASTROENTEROLOGY,2008,14(21):3421-3424.
APA	Bu XianMin,Zhao ChengHai,Zhang Ning,Gao Feng,Lin Shuai,&Dai XianWei.(2008).Hypermethylation and aberrant expression of secreted fizzled-related protein genes in pancreatic cancer.WORLD JOURNAL OF GASTROENTEROLOGY,14(21),3421-3424.
MLA	Bu XianMin,et al."Hypermethylation and aberrant expression of secreted fizzled-related protein genes in pancreatic cancer".WORLD JOURNAL OF GASTROENTEROLOGY 14.21(2008):3421-3424.