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Hypermethylation and aberrant expression of secreted fizzled-related protein genes in pancreatic cancer
Alternative TitleHypermethylation and aberrant expression of secreted fizzled-related protein genes in pancreatic cancer
Bu XianMin; Zhao ChengHai; Zhang Ning; Gao Feng; Lin Shuai; Dai XianWei
2008
Source PublicationWORLD JOURNAL OF GASTROENTEROLOGY
ISSN1007-9327
Volume14Issue:21Pages:3421-3424
AbstractAIM: To determine the methylation status and aberrant expression of some secreted frizzled-related protein (SFRP) genes in pancreatic cancer and explore their role in pancreatic carcinogenesis.
Other AbstractAIM:To determine the methylation status and aberrant expression of some secreted frizzled-related protein (SFRP) genes in pancreatic cancer and explore their role in pancreatic carcinogenesis. METHODS:Methylation status and expression of SFRP genes were detected by methylation-specific PCR (MSPCR) and reverse-transcription PCR (RT-PCR) respectively. RESULTS:The frequencies of methylation for SFRP genes 1,2,4,5 were 70%, 48.3%,60% and 76.7% in pancreatic cancer samples, and 21.7%, 20%,10% and 36.7% in matched cancer adjacent normal tissue samples,respectively (χ2=28.23,P<0.0001 for SFRP gene 1; χ2=10.71,P=0.001 for SFRP gene 2;χ2=32.97,P<0.0001 for SFRP gene 4;χ2=19.55,P<0.0001 for SFRP gene 5). Expression loss of SFRP genes 1,2,4 and 5 was found in 65%,40%,55% and 71.7% of 60 pancreatic cancer samples, and 25%,15%,18.3% and 31.7% of matched cancer adjacent normal tissue samples,respectively (χ2=19.39,P<0.0001 for SFRP gene 1;χ2=9.40,P=0.002 for SFRP gene 2;χ2=17.37,P<0.0001 for SFRP gene 4;χ2=19.22,P<0.0001 for SFRP gene 5).SFRP gene 1 was methylated but not expressed in PC-3 and PANC-1,SFRP gene 2 was methylated but not expressed in PANC-1 and CFPAC-1,SFRP gene 4 was methylated but not expressed in PC-3,and SFRP gene 5 was methylated but not expressed in CFPAC-1. CONCLUSION:Hypermethylation and aberrant expression of SFRP genes are common in pancreatic cancer,which may be involved in pancreatic carcinogenesis.
KeywordWNT ANTAGONIST SFRP1 BETA-ESTRADIOL UP-REGULATION MCF-7 CELLS WNT/BETA-CATENIN PROSTATE-CANCER TUMOR ADENOCARCINOMA METHYLATION TUMORIGENESIS hypermethylation secreted frizzled-related protein pancreatic cancer
Indexed ByCSCD
Language英语
CSCD IDCSCD:3317612
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Document Type期刊论文
Identifierhttp://ir.imr.ac.cn/handle/321006/157967
Collection中国科学院金属研究所
Affiliation中国科学院金属研究所
Recommended Citation
GB/T 7714
Bu XianMin,Zhao ChengHai,Zhang Ning,et al. Hypermethylation and aberrant expression of secreted fizzled-related protein genes in pancreatic cancer[J]. WORLD JOURNAL OF GASTROENTEROLOGY,2008,14(21):3421-3424.
APA Bu XianMin,Zhao ChengHai,Zhang Ning,Gao Feng,Lin Shuai,&Dai XianWei.(2008).Hypermethylation and aberrant expression of secreted fizzled-related protein genes in pancreatic cancer.WORLD JOURNAL OF GASTROENTEROLOGY,14(21),3421-3424.
MLA Bu XianMin,et al."Hypermethylation and aberrant expression of secreted fizzled-related protein genes in pancreatic cancer".WORLD JOURNAL OF GASTROENTEROLOGY 14.21(2008):3421-3424.
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