IMR OpenIR
The Effects of Lewis(y) Antigenic Content on Drug Resistance to Carboplatin in Ovarian Cancer Line RMG- I
Alternative TitleThe Effects of Lewis(y) Antigenic Content on Drug Resistance to Carboplatin in Ovarian Cancer Line RMG- I
Zhao Yue1; Lin Bei1; Hao YingYing1; Yan LiMei1; Liu JuanJuan1; Zhu LianCheng1; Mang ShuLan1
2008
Source PublicationPROGRESS IN BIOCHEMISTRY AND BIOPHYSICS
ISSN1000-3282
Volume35Issue:10Pages:1175-1182
AbstractTo investigate the relationship between the changes of Lewis (y) antigenic content and the drug resistance of the cells to carboplatin in ovarian cancer lines with RMG- I -H which expresseed higher Lewis(y) antigen on cell surface stably. RMG- I was one kind of epithelial ovarian cancer cell lines. Cell lines RMG- I -C and RMG- I -H were obtained from RMG- I which were transfected with keno-plasmid and alpha 1, 2-fucosyltransferase gene, respectively. The method of methyl thiazolyl tetrazolium (MTT) was used to determine the fractional inhibition ratio (IR) of the three cell lines which were affected by carboplatin in different concentrations. Accordingly, the IC50 of the three cell lines could be calculated. Carboplatin induced apoptosis of three cell lines was observed by flow cytometry (FCM). In addition, the apoptotic ratios of the three lines that were treated by 30 mg/L and 60 mg/L carboplatin were measured by the FCM at the same time. And then, the apoptosis conditions of the three lines were observed by the methods of fluorescent staining and transmission electron microscope (TEM). The IC50 of RMG- I -H was (58.07 +/- 2.42), which was obviously higher than that of RMG- I (28.83 +/- 3.57) and RMG- I -C(25.71 +/- 8.24), all showed a significant statistical difference (P 0.05). FCM analysis confirmed the cell apoptosis in the three lines. The corr-apoptotic ratios of RMG- I -H treated with 3 0 mg/L and 60 mg/L carboplatin were (20.43 +/- 0.7 1)% and (38.11 +/- 0.33)%, respectively, which were about 49%similar to 63% of the RMG- I (35.87 +/- 3.84)%, (63.37 +/- 9.59)% and RMG- I -C(34.80 +/- 3.59)%, (60.17 +/- 6.64)%, both presented a statistical difference (P 0.05). The apoptotic degree of RMG- I -H was lower than that of the other cell lines in the same carboplatin concentration with the method of inv-fluorescence microscopy and transmission electron microscopy (TEM). The drug resistance of ovarian cancer cells to carboplatin was stronger when the Lewis(y) antigenic contents on cell surface increased.
KeywordP-GLYCOPROTEIN EXPRESSION CELLS ovarian cancer Lewis(y) antigen carboplatin drug resistance
Indexed ByCSCD
Language英语
Funding Project[The National Natural Science Foundation of China] ; [Liaoning Province Doctor Startup Fund] ; [The Educational Department Science foundation of Liaoning Province] ; [Liaoning Natural Science Foundation]
CSCD IDCSCD:3420673
Citation statistics
Cited Times:5[CSCD]   [CSCD Record]
Document Type期刊论文
Identifierhttp://ir.imr.ac.cn/handle/321006/158049
Collection中国科学院金属研究所
Affiliation1.中国科学院金属研究所
2.Dalian Med University, Affiliated Hosp 2, Dept Obstet & Gynecol, Dalian 116021, Peoples R China
Recommended Citation
GB/T 7714
Zhao Yue,Lin Bei,Hao YingYing,et al. The Effects of Lewis(y) Antigenic Content on Drug Resistance to Carboplatin in Ovarian Cancer Line RMG- I[J]. PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS,2008,35(10):1175-1182.
APA Zhao Yue.,Lin Bei.,Hao YingYing.,Yan LiMei.,Liu JuanJuan.,...&Mang ShuLan.(2008).The Effects of Lewis(y) Antigenic Content on Drug Resistance to Carboplatin in Ovarian Cancer Line RMG- I.PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS,35(10),1175-1182.
MLA Zhao Yue,et al."The Effects of Lewis(y) Antigenic Content on Drug Resistance to Carboplatin in Ovarian Cancer Line RMG- I".PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS 35.10(2008):1175-1182.
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Zhao Yue]'s Articles
[Lin Bei]'s Articles
[Hao YingYing]'s Articles
Baidu academic
Similar articles in Baidu academic
[Zhao Yue]'s Articles
[Lin Bei]'s Articles
[Hao YingYing]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Zhao Yue]'s Articles
[Lin Bei]'s Articles
[Hao YingYing]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.