The Effects of Lewis(y) Antigenic Content on Drug Resistance to Carboplatin in Ovarian Cancer Line RMG- I

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	The Effects of Lewis(y) Antigenic Content on Drug Resistance to Carboplatin in Ovarian Cancer Line RMG- I
其他题名	The Effects of Lewis（y） Antigenic Content on Drug Resistance to Carboplatin in Ovarian Cancer Line RMG- I
	Zhao Yue 1; Lin Bei 1; Hao YingYing 1; Yan LiMei 1; Liu JuanJuan 1; Zhu LianCheng 1; Mang ShuLan 1
	2008
发表期刊	PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS
ISSN	1000-3282
卷号	35 期号:10 页码:1175-1182
摘要	To investigate the relationship between the changes of Lewis (y) antigenic content and the drug resistance of the cells to carboplatin in ovarian cancer lines with RMG- I -H which expresseed higher Lewis(y) antigen on cell surface stably. RMG- I was one kind of epithelial ovarian cancer cell lines. Cell lines RMG- I -C and RMG- I -H were obtained from RMG- I which were transfected with keno-plasmid and alpha 1, 2-fucosyltransferase gene, respectively. The method of methyl thiazolyl tetrazolium (MTT) was used to determine the fractional inhibition ratio (IR) of the three cell lines which were affected by carboplatin in different concentrations. Accordingly, the IC50 of the three cell lines could be calculated. Carboplatin induced apoptosis of three cell lines was observed by flow cytometry (FCM). In addition, the apoptotic ratios of the three lines that were treated by 30 mg/L and 60 mg/L carboplatin were measured by the FCM at the same time. And then, the apoptosis conditions of the three lines were observed by the methods of fluorescent staining and transmission electron microscope (TEM). The IC50 of RMG- I -H was (58.07 +/- 2.42), which was obviously higher than that of RMG- I (28.83 +/- 3.57) and RMG- I -C(25.71 +/- 8.24), all showed a significant statistical difference (P 0.05). FCM analysis confirmed the cell apoptosis in the three lines. The corr-apoptotic ratios of RMG- I -H treated with 3 0 mg/L and 60 mg/L carboplatin were (20.43 +/- 0.7 1)% and (38.11 +/- 0.33)%, respectively, which were about 49%similar to 63% of the RMG- I (35.87 +/- 3.84)%, (63.37 +/- 9.59)% and RMG- I -C(34.80 +/- 3.59)%, (60.17 +/- 6.64)%, both presented a statistical difference (P 0.05). The apoptotic degree of RMG- I -H was lower than that of the other cell lines in the same carboplatin concentration with the method of inv-fluorescence microscopy and transmission electron microscopy (TEM). The drug resistance of ovarian cancer cells to carboplatin was stronger when the Lewis(y) antigenic contents on cell surface increased.
关键词	P-GLYCOPROTEIN EXPRESSION CELLS ovarian cancer Lewis(y) antigen carboplatin drug resistance
收录类别	CSCD
语种	英语
资助项目	[The National Natural Science Foundation of China] ; [Liaoning Province Doctor Startup Fund] ; [The Educational Department Science foundation of Liaoning Province] ; [Liaoning Natural Science Foundation]
CSCD记录号	CSCD:3420673
引用统计	被引频次：5[CSCD] [CSCD记录]
文献类型	期刊论文
条目标识符	http://ir.imr.ac.cn/handle/321006/158049
专题	中国科学院金属研究所
作者单位	1.中国科学院金属研究所 2.Dalian Med University, Affiliated Hosp 2, Dept Obstet & Gynecol, Dalian 116021, Peoples R China
推荐引用方式 GB/T 7714	Zhao Yue,Lin Bei,Hao YingYing,et al. The Effects of Lewis(y) Antigenic Content on Drug Resistance to Carboplatin in Ovarian Cancer Line RMG- I[J]. PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS,2008,35(10):1175-1182.
APA	Zhao Yue.,Lin Bei.,Hao YingYing.,Yan LiMei.,Liu JuanJuan.,...&Mang ShuLan.(2008).The Effects of Lewis(y) Antigenic Content on Drug Resistance to Carboplatin in Ovarian Cancer Line RMG- I.PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS,35(10),1175-1182.
MLA	Zhao Yue,et al."The Effects of Lewis(y) Antigenic Content on Drug Resistance to Carboplatin in Ovarian Cancer Line RMG- I".PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS 35.10(2008):1175-1182.