Effects of Oxytetracycline on Methane Production and the Microbial Communities During Anaerobic Digestion of Cow Manure

IMR OpenIR

	Effects of Oxytetracycline on Methane Production and the Microbial Communities During Anaerobic Digestion of Cow Manure
其他题名	Effects of Oxytetracycline on Methane Production and the Microbial Communities During Anaerobic Digestion of Cow Manure
	Ke Xin; Wang Chunyong; Li Rundong; Zhang Yun
	2014
发表期刊	JOURNAL OF INTEGRATIVE AGRICULTURE
ISSN	2095-3119
卷号	13 期号:6 页码:1373-1381
摘要	The effects of different concentrations of oxytetracycline (OTC) on the dynamics of bacterial and archaeal communities during the mesophilic anaerobic digestion (37 degrees C) of cow manure were investigated. Before anaerobic digestion, OTC was added to digesters at concentrations of 20, 50, and 80 mg L-1, respectively. Compared with no-antibiotic control, all methane productions underwent different levels of inhibition at different concentrations of OTC. Changes in the bacterial and archaeal communities were discussed by using PCR-denaturing gradient gel electrophoresis (DGGE). Results showed that OTC affected the richness and diversity of bacterial and archaeal communities. The bacterial genus Flavobacterium and an uncultured bacterium (JN256083.1) were detected throughout the entire process of anaerobic digestion and seemed to be the functional bacteria. Methanobrevibacter boviskoreani and an uncultured archaeon (FJ230982.1) dominated the archaeal communities during anaerobic digestion. These microorganisms may have high resistance to OTC and may play vital roles in methane production.
其他摘要	The effects of different concentrations of oxytetracycline (OTC) on the dynamics of bacterial and archaeal communities during the mesophilic anaerobic digestion (37°C) of cow manure were investigated. Before anaerobic digestion, OTC was added to digesters at concentrations of 20, 50, and 80 mg L~(-1), respectively. Compared with no-antibiotic control, all methane productions underwent different levels of inhibition at different concentrations of OTC. Changes in the bacterial and archaeal communities were discussed by using PCR-denaturing gradient gel electrophoresis (DGGE). Results showed that OTC affected the richness and diversity of bacterial and archaeal communities. The bacterial genus Flavobacterium and an uncultured bacterium (JN256083.1) were detected throughout the entire process of anaerobic digestion and seemed to be the functional bacteria. Methanobrevibacter boviskoreani and an uncultured archaeon (FJ230982.1) dominated the archaeal communities during anaerobic digestion. These microorganisms may have high resistance to OTC and may play vital roles in methane production.
关键词	RESISTANCE GENES BIOGAS PRODUCTION ANTIBIOTICS DIVERSITY FATE CHROMATOGRAPHY BACTERIAL SLURRY CHINA oxytetracycline anaerobic digestion stages (BDS, DMS and FDS) PCR-DGGE bacterial community archaeal community
收录类别	CSCD
语种	英语
资助项目	[Major Science and Technology Program for Water Pollution Control and Treatment, China]
CSCD记录号	CSCD:5169168
引用统计	被引频次：4[CSCD] [CSCD记录]
文献类型	期刊论文
条目标识符	http://ir.imr.ac.cn/handle/321006/158169
专题	中国科学院金属研究所
作者单位	中国科学院金属研究所
推荐引用方式 GB/T 7714	Ke Xin,Wang Chunyong,Li Rundong,et al. Effects of Oxytetracycline on Methane Production and the Microbial Communities During Anaerobic Digestion of Cow Manure[J]. JOURNAL OF INTEGRATIVE AGRICULTURE,2014,13(6):1373-1381.
APA	Ke Xin,Wang Chunyong,Li Rundong,&Zhang Yun.(2014).Effects of Oxytetracycline on Methane Production and the Microbial Communities During Anaerobic Digestion of Cow Manure.JOURNAL OF INTEGRATIVE AGRICULTURE,13(6),1373-1381.
MLA	Ke Xin,et al."Effects of Oxytetracycline on Methane Production and the Microbial Communities During Anaerobic Digestion of Cow Manure".JOURNAL OF INTEGRATIVE AGRICULTURE 13.6(2014):1373-1381.