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Antibacterial effect of a copper-containing titanium alloy against implant-associated infection induced by methicillin-resistant Staphylococcus aureus
Zhuang, Yifu1; Ren, Ling2; Zhang, Shuyuan2; Wei, Xiang3; Yang, Ke2; Dai, Kerong1
Corresponding AuthorYang, Ke(kyang@imr.ac.cn) ; Dai, Kerong(krdai@163.com)
2021
Source PublicationACTA BIOMATERIALIA
ISSN1742-7061
Volume119Pages:472-484
AbstractImplant-associated infection (IAI) induced by methicillin-resistant Staphylococcus aureus (MRSA) is a devastating complication in the orthopedic clinic. Traditional implant materials, such as Ti6Al4V, are vulnerable to microbial infection. In this study, we fabricated a copper (Cu)-containing titanium alloy (Ti6Al4V-Cu) for the prevention and treatment of MRSA-induced IAI. The material characteristics, antibacterial activity, and biocompatibility of Ti6Al4V-Cu were systematically investigated and compared with those of Ti6Al4V. Ti6Al4V-Cu provided stable and continuous Cu2+ release, at a rate of 0.106 mg/cm(2)/d. Its antibacterial performance against MRSA in vitro was confirmed by plate counting analysis, crystal violet staining, and scanning electron microscopic observations. Reverse transcription quantitative polymerase chain reaction (RT-qPCR) analysis demonstrated that Ti6Al4V-Cu suppressed biofilm formation, virulence, and antibioticresistance of MRSA. The in vivo anti-MRSA effect was investigated in a rat IAI model. Implants were contaminated with MRSA solution, implanted into the femur, and left for 6 weeks. Severe IAI developed in the Ti6Al4V group, with increased radiological score (9.6 +/- 1.3) and high histological score (10.1 +/- 1.9). However, no sign of infection was found in the Ti6Al4V-Cu group, as indicated by decreased radiological score (1.3 +/- 0.4) and low histological score (2.3 +/- 0.5). In addition, Ti6Al4V-Cu had favorable biocompatibility both in vitro and in vivo . In summary, Ti6Al4V-Cu is a promising implant material to protect against MRSA-induced IAI. (C) 2020 Published by Elsevier Ltd on behalf of Acta Materialia Inc.
KeywordAntibacterial effect Copper Titanium alloy Implant-associated infection MRSA
Funding OrganizationNational Natural Science Foundation of China
DOI10.1016/j.actbio.2020.10.026
Indexed BySCI
Language英语
Funding ProjectNational Natural Science Foundation of China[51631009] ; National Natural Science Foundation of China[51931001]
WOS Research AreaEngineering ; Materials Science
WOS SubjectEngineering, Biomedical ; Materials Science, Biomaterials
WOS IDWOS:000602981500004
PublisherELSEVIER SCI LTD
Citation statistics
Document Type期刊论文
Identifierhttp://ir.imr.ac.cn/handle/321006/158826
Collection中国科学院金属研究所
Corresponding AuthorYang, Ke; Dai, Kerong
Affiliation1.Shanghai Jiao Tong Univ, Shanghai Peoples Hosp 9, Dept Orthopaed Surg, Shanghai Key Lab Orthopaed Implants,Sch Med, Shanghai, Peoples R China
2.Chinese Acad Sci, Inst Met Res, 72 Wenhua Rd, Shenyang 110016, Peoples R China
3.Suzhou Silvan Med Co Ltd, Suzhou, Peoples R China
Recommended Citation
GB/T 7714
Zhuang, Yifu,Ren, Ling,Zhang, Shuyuan,et al. Antibacterial effect of a copper-containing titanium alloy against implant-associated infection induced by methicillin-resistant Staphylococcus aureus[J]. ACTA BIOMATERIALIA,2021,119:472-484.
APA Zhuang, Yifu,Ren, Ling,Zhang, Shuyuan,Wei, Xiang,Yang, Ke,&Dai, Kerong.(2021).Antibacterial effect of a copper-containing titanium alloy against implant-associated infection induced by methicillin-resistant Staphylococcus aureus.ACTA BIOMATERIALIA,119,472-484.
MLA Zhuang, Yifu,et al."Antibacterial effect of a copper-containing titanium alloy against implant-associated infection induced by methicillin-resistant Staphylococcus aureus".ACTA BIOMATERIALIA 119(2021):472-484.
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