IMR OpenIR
Copper-Containing Alloy as Immunoregulatory Material in Bone Regeneration via Mitochondrial Oxidative Stress
Xu, Daorong1,2; Qian, Jikun1,2; Guan, Xin1,2; Ren, Ling3; Yang, Kaifan1,2; Huang, Xuan4; Zhang, Shuyuan3; Chai, Yu1,2; Wu, Xiaohu1,2; Wu, Hangtian1,2; Zhang, Xianrong1,2; Yang, Ke3; Yu, Bin1,2
Corresponding AuthorZhang, Xianrong(xianrongzh@smu.edu.cn) ; Yang, Ke(kyang@imr.ac.cn) ; Yu, Bin(yubin@smu.edu.cn)
2021-01-14
Source PublicationFRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
ISSN2296-4185
Volume8Pages:12
AbstractIn the mammalian skeletal system, osteogenesis and angiogenesis are closely linked by type H vessels during bone regeneration and repair. Our previous studies confirmed the promotion of these processes by copper-containing metal (CCM) in vitro and in vivo. However, whether and how the coupling of angiogenesis and osteogenesis participates in the promotion of bone regeneration by CCM in vivo is unknown. In this study, M2a macrophages but not M2c macrophages were shown to be immunoregulated by CCM. A CCM, 316L-5Cu, was applied to drilling hole injuries of the tibia of C57/6 mice for comparison. We observed advanced formation of cortical bone and type H vessels beneath the new bone in the 316L-5Cu group 14 and 21 days postinjury. Moreover, the recruitment of CD206-positive M2a macrophages, which are regarded as the primary source of platelet-derived growth factor type BB (PDGF-BB), was significantly promoted at the injury site at days 14 and 21. Under the stimulation of CCM, mitochondria-derived reactive oxygen species were also found to be upregulated in CD206(hi) M2a macrophages in vitro, and this upregulation was correlated with the expression of PDGF-BB. In conclusion, our results indicate that CCM promotes the evolution of callus through the generation of type H vessels during the process of bone repair by upregulating the expression of PDGF-BB derived from M2a macrophages.
Keyword316-5Cu stainless steel type H vessel M2a macrophage PDGF-BB immunoregulation
Funding OrganizationMajor Program of National Natural Science Foundation of China ; National Natural Science Foundation of China
DOI10.3389/fbioe.2020.620629
Indexed BySCI
Language英语
Funding ProjectMajor Program of National Natural Science Foundation of China[81830079] ; National Natural Science Foundation of China[31870954] ; National Natural Science Foundation of China[81702009]
WOS Research AreaBiotechnology & Applied Microbiology ; Science & Technology - Other Topics
WOS SubjectBiotechnology & Applied Microbiology ; Multidisciplinary Sciences
WOS IDWOS:000615949700001
PublisherFRONTIERS MEDIA SA
Citation statistics
Document Type期刊论文
Identifierhttp://ir.imr.ac.cn/handle/321006/159163
Collection中国科学院金属研究所
Corresponding AuthorZhang, Xianrong; Yang, Ke; Yu, Bin
Affiliation1.Southern Med Univ, Nanfang Hosp, Div Orthoped Surg, Dept Orthoped, Guangzhou, Peoples R China
2.Southern Med Univ, Nanfang Hosp, Guangdong Prov Key Lab Bone & Cartilage Regenerat, Guangzhou, Peoples R China
3.Chinese Acad Sci, Inst Met Res, Shenyang, Peoples R China
4.Southern Med Univ, Nanfang Hosp, Dept Infect Dis, State Key Lab Organ Failure Res,Guangdong Prov Ke, Guangzhou, Peoples R China
Recommended Citation
GB/T 7714
Xu, Daorong,Qian, Jikun,Guan, Xin,et al. Copper-Containing Alloy as Immunoregulatory Material in Bone Regeneration via Mitochondrial Oxidative Stress[J]. FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY,2021,8:12.
APA Xu, Daorong.,Qian, Jikun.,Guan, Xin.,Ren, Ling.,Yang, Kaifan.,...&Yu, Bin.(2021).Copper-Containing Alloy as Immunoregulatory Material in Bone Regeneration via Mitochondrial Oxidative Stress.FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY,8,12.
MLA Xu, Daorong,et al."Copper-Containing Alloy as Immunoregulatory Material in Bone Regeneration via Mitochondrial Oxidative Stress".FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY 8(2021):12.
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Xu, Daorong]'s Articles
[Qian, Jikun]'s Articles
[Guan, Xin]'s Articles
Baidu academic
Similar articles in Baidu academic
[Xu, Daorong]'s Articles
[Qian, Jikun]'s Articles
[Guan, Xin]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Xu, Daorong]'s Articles
[Qian, Jikun]'s Articles
[Guan, Xin]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.