Copper-Containing Alloy as Immunoregulatory Material in Bone Regeneration via Mitochondrial Oxidative Stress

doi:10.3389/fbioe.2020.620629

IMR OpenIR

	Copper-Containing Alloy as Immunoregulatory Material in Bone Regeneration via Mitochondrial Oxidative Stress
	Xu, Daorong 1,2; Qian, Jikun 1,2; Guan, Xin 1,2; Ren, Ling 3; Yang, Kaifan 1,2; Huang, Xuan 4; Zhang, Shuyuan 3; Chai, Yu 1,2; Wu, Xiaohu 1,2; Wu, Hangtian 1,2; Zhang, Xianrong 1,2; Yang, Ke 3; Yu, Bin 1,2
通讯作者	Zhang, Xianrong(xianrongzh@smu.edu.cn) ; Yang, Ke(kyang@imr.ac.cn) ; Yu, Bin(yubin@smu.edu.cn)
	2021-01-14
发表期刊	FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
ISSN	2296-4185
卷号	8 页码:12
摘要	In the mammalian skeletal system, osteogenesis and angiogenesis are closely linked by type H vessels during bone regeneration and repair. Our previous studies confirmed the promotion of these processes by copper-containing metal (CCM) in vitro and in vivo. However, whether and how the coupling of angiogenesis and osteogenesis participates in the promotion of bone regeneration by CCM in vivo is unknown. In this study, M2a macrophages but not M2c macrophages were shown to be immunoregulated by CCM. A CCM, 316L-5Cu, was applied to drilling hole injuries of the tibia of C57/6 mice for comparison. We observed advanced formation of cortical bone and type H vessels beneath the new bone in the 316L-5Cu group 14 and 21 days postinjury. Moreover, the recruitment of CD206-positive M2a macrophages, which are regarded as the primary source of platelet-derived growth factor type BB (PDGF-BB), was significantly promoted at the injury site at days 14 and 21. Under the stimulation of CCM, mitochondria-derived reactive oxygen species were also found to be upregulated in CD206(hi) M2a macrophages in vitro, and this upregulation was correlated with the expression of PDGF-BB. In conclusion, our results indicate that CCM promotes the evolution of callus through the generation of type H vessels during the process of bone repair by upregulating the expression of PDGF-BB derived from M2a macrophages.
关键词	316-5Cu stainless steel type H vessel M2a macrophage PDGF-BB immunoregulation
资助者	Major Program of National Natural Science Foundation of China ; National Natural Science Foundation of China
DOI	10.3389/fbioe.2020.620629
收录类别	SCI
语种	英语
资助项目	Major Program of National Natural Science Foundation of China[81830079] ; National Natural Science Foundation of China[31870954] ; National Natural Science Foundation of China[81702009]
WOS研究方向	Biotechnology & Applied Microbiology ; Science & Technology - Other Topics
WOS类目	Biotechnology & Applied Microbiology ; Multidisciplinary Sciences
WOS记录号	WOS:000615949700001
出版者	FRONTIERS MEDIA SA
引用统计	被引频次：15[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.imr.ac.cn/handle/321006/159163
专题	中国科学院金属研究所
通讯作者	Zhang, Xianrong; Yang, Ke; Yu, Bin
作者单位	1.Southern Med Univ, Nanfang Hosp, Div Orthoped Surg, Dept Orthoped, Guangzhou, Peoples R China 2.Southern Med Univ, Nanfang Hosp, Guangdong Prov Key Lab Bone & Cartilage Regenerat, Guangzhou, Peoples R China 3.Chinese Acad Sci, Inst Met Res, Shenyang, Peoples R China 4.Southern Med Univ, Nanfang Hosp, Dept Infect Dis, State Key Lab Organ Failure Res,Guangdong Prov Ke, Guangzhou, Peoples R China
推荐引用方式 GB/T 7714	Xu, Daorong,Qian, Jikun,Guan, Xin,et al. Copper-Containing Alloy as Immunoregulatory Material in Bone Regeneration via Mitochondrial Oxidative Stress[J]. FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY,2021,8:12.
APA	Xu, Daorong.,Qian, Jikun.,Guan, Xin.,Ren, Ling.,Yang, Kaifan.,...&Yu, Bin.(2021).Copper-Containing Alloy as Immunoregulatory Material in Bone Regeneration via Mitochondrial Oxidative Stress.FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY,8,12.
MLA	Xu, Daorong,et al."Copper-Containing Alloy as Immunoregulatory Material in Bone Regeneration via Mitochondrial Oxidative Stress".FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY 8(2021):12.