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Improved activity of MC3T3-E1 cells by the exciting piezoelectric BaTiO3/TC4 using low-intensity pulsed ultrasound
Cai, Kunzhan1; Jiao, Yilai2; Quan, Quan1; Hao, Yulin2; Liu, Jie3; Wu, Lin1
Corresponding AuthorWu, Lin(wulin13@163.com)
2021-11-01
Source PublicationBIOACTIVE MATERIALS
Volume6Issue:11Pages:4073-4082
AbstractDeveloping bioactive materials for bone implants to enhance bone healing and bone growth has for years been the focus of clinical research. Barium titanate (BT) is an electroactive material that can generate electrical signals in response to applied mechanical forces. In this study, a BT piezoelectric ceramic coating was synthesized on the surface of a TC4 titanium alloy, forming a BT/TC4 material, and low-intensity pulsed ultrasound (LIPUS) was then applied as a mechanical stimulus. The combined effects on the biological responses of MC3T3-E1 cells were investigated. Results of scanning electron microscopy, energy-dispersive X-ray spectroscopy, and X-ray diffraction showed that an uniform nanospheres -shaped BT coating was formed on TC4 substrate. Piezoelectric behaviors were observed using piezoelectric force microscopy with the piezoelectric coefficient d(33) of 0.42 pC/N. Electrochemical measures indicated that LIPUS-stimulated BT/TC4 materials could produce a microcurrent of approximately 10 mu A/cm(2). In vitro, the greatest osteogenesis (cell adhesion, proliferation, and osteogenic differentiation) was found in MC3T3-E1 cells when BT/TC4 was stimulated using LIPUS. Furthermore, the intracellular calcium ion concentration increased in these cells, possibly because opening of the L-type calcium ion channels was promoted and expression of the Ca(V)1.2 protein was increased. Therefore, the piezoelectric BT/TC4 material with LIPUS loading synergistically promoted osteogenesis, rending it a potential treatment for early stage formation of reliable bone-implant contact.
KeywordBarium titanate Low-intensity pulsed ultrasound Osteogenesis Piezoelectric effect
Funding OrganizationNational Natural Science Foundation of China ; Natural Science Foundation of Liaoning Province, China ; Scientists Partner Project of China Medical University-Shenyang Branch of Chinese Academy of Sciences
DOI10.1016/j.bioactmat.2021.04.016
Indexed BySCI
Language英语
Funding ProjectNational Natural Science Foundation of China[81870811] ; Natural Science Foundation of Liaoning Province, China[20180530082] ; Scientists Partner Project of China Medical University-Shenyang Branch of Chinese Academy of Sciences[HZHB2018017]
WOS Research AreaEngineering ; Materials Science
WOS SubjectEngineering, Biomedical ; Materials Science, Biomaterials
WOS IDWOS:000685107200030
PublisherKEAI PUBLISHING LTD
Citation statistics
Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.imr.ac.cn/handle/321006/159236
Collection中国科学院金属研究所
Corresponding AuthorWu, Lin
Affiliation1.China Med Univ, Sch & Hosp Stomatol, Liaoning Prov Key Lab Oral Dis, Shenyang 110001, Peoples R China
2.Chinese Acad Sci, Shenyang Natl Lab Mat Sci, Inst Met Res, Shenyang 110016, Peoples R China
3.China Med Univ, Dept Sci Expt Ctr, Shenyang 110122, Peoples R China
Recommended Citation
GB/T 7714
Cai, Kunzhan,Jiao, Yilai,Quan, Quan,et al. Improved activity of MC3T3-E1 cells by the exciting piezoelectric BaTiO3/TC4 using low-intensity pulsed ultrasound[J]. BIOACTIVE MATERIALS,2021,6(11):4073-4082.
APA Cai, Kunzhan,Jiao, Yilai,Quan, Quan,Hao, Yulin,Liu, Jie,&Wu, Lin.(2021).Improved activity of MC3T3-E1 cells by the exciting piezoelectric BaTiO3/TC4 using low-intensity pulsed ultrasound.BIOACTIVE MATERIALS,6(11),4073-4082.
MLA Cai, Kunzhan,et al."Improved activity of MC3T3-E1 cells by the exciting piezoelectric BaTiO3/TC4 using low-intensity pulsed ultrasound".BIOACTIVE MATERIALS 6.11(2021):4073-4082.
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