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Long non-coding RNA AFAP1-AS1 accelerates the progression of melanoma by targeting miR-653-5p
Alternative TitleLong non-coding RNA AFAP1-AS1 accelerates the progression of melanoma by targeting miR-653-5p
Fei Liu1; Lanting Hu2; Yi Pei1; Ke Zheng1; Wei Wang1; Shenglong Li1; Enduo Qiu1; Guanning Shang1; Jiaming Zhang1; Xiaojing Zhang1
2020-01-01
Source PublicationBMC Cancer
ISSN1471-2407
Volume20Issue:1
AbstractBackground Melanoma is the most aggressive skin cancer that derived from pigment cells, accounting for the majority of the skin-cancer-related deaths. Despite great development and evolution have been made in surgery, radiotherapy and adjuvant chemotherapy, the prognosis of melanoma patients exhibited no significant improvement. Long noncoding RNAs (lncRNAs) are frequently dysregulated and involved in the development of cancers. LncRNA AFAP1-AS1 has been explored in various cancers, whereas its role and regulatory mechanism in melanoma are not well understood. Methods The expression of AFAP1-AS1 was detected by qRT-PCR. CCK-8, colony formation, transwell and western blot assays were performed to investigate the biological role of AFAP1-AS1 in melanoma. Male BALB Results AFAP1-AS1 was highly expressed in melanoma cell lines. Suppression of AFAP1-AS1 impaired cell proliferation, migration, invasion and EMT in melanoma. Moreover, AFAP1-AS1 was a ceRNA of RAI14 by competitively binding with miR-653-5p. Besides, miR-653-5p overexpression or RAI14 inhibition could repress tumor growth. Eventually, rescue assays indicated that the function of AFAP1-AS1 in the cellular process of melanoma was dependent on miR-653-5p and RAI14. Conclusions AFAP1-AS1 exerts its oncogenic function in melanoma by targeting miR-653-5p
Other AbstractBackground Melanoma is the most aggressive skin cancer that derived from pigment cells, accounting for the majority of the skin-cancer-related deaths. Despite great development and evolution have been made in surgery, radiotherapy and adjuvant chemotherapy, the prognosis of melanoma patients exhibited no significant improvement. Long noncoding RNAs (lncRNAs) are frequently dysregulated and involved in the development of cancers. LncRNA AFAP1-AS1 has been explored in various cancers, whereas its role and regulatory mechanism in melanoma are not well understood. Methods The expression of AFAP1-AS1 was detected by qRT-PCR. CCK-8, colony formation, transwell and western blot assays were performed to investigate the biological role of AFAP1-AS1 in melanoma. Male BALB Results AFAP1-AS1 was highly expressed in melanoma cell lines. Suppression of AFAP1-AS1 impaired cell proliferation, migration, invasion and EMT in melanoma. Moreover, AFAP1-AS1 was a ceRNA of RAI14 by competitively binding with miR-653-5p. Besides, miR-653-5p overexpression or RAI14 inhibition could repress tumor growth. Eventually, rescue assays indicated that the function of AFAP1-AS1 in the cellular process of melanoma was dependent on miR-653-5p and RAI14. Conclusions AFAP1-AS1 exerts its oncogenic function in melanoma by targeting miR-653-5p
Language英语
Document Type期刊论文
Identifierhttp://ir.imr.ac.cn/handle/321006/159343
Collection中国科学院金属研究所
Affiliation1.中国医科大学
2.Institute of Metal Research, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Fei Liu,Lanting Hu,Yi Pei,et al. Long non-coding RNA AFAP1-AS1 accelerates the progression of melanoma by targeting miR-653-5p[J]. BMC Cancer,2020,20(1).
APA Fei Liu.,Lanting Hu.,Yi Pei.,Ke Zheng.,Wei Wang.,...&Xiaojing Zhang.(2020).Long non-coding RNA AFAP1-AS1 accelerates the progression of melanoma by targeting miR-653-5p.BMC Cancer,20(1).
MLA Fei Liu,et al."Long non-coding RNA AFAP1-AS1 accelerates the progression of melanoma by targeting miR-653-5p".BMC Cancer 20.1(2020).
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