Biomimetic inorganic-organic hybrid nanoparticles from magnesium-substituted amorphous calcium phosphate clusters and polyacrylic acid molecules

doi:10.1016/j.bioactmat.2021.01.005

IMR OpenIR

	Biomimetic inorganic-organic hybrid nanoparticles from magnesium-substituted amorphous calcium phosphate clusters and polyacrylic acid molecules
	Li, Na 1,2; Cui, Wei 1,2; Cong, Peifang 3; Tang, Jie 5; Guan, Yong 4; Huang, Caihao 1,2; Liu, Yunen 3; Yu, Chengzhong 5; Yang, Rui 1,2; Zhang, Xing 1,2
通讯作者	Zhang, Xing(xingzhang@imr.ac.cn)
	2021-08-01
发表期刊	BIOACTIVE MATERIALS
卷号	6 期号:8 页码:2303-2314
摘要	Amorphous calcium phosphate (ACP) has been widely found during bone and tooth biomineralization, but the meta-stability and labile nature limit further biomedical applications. The present study found that the chelation of polyacrylic acid (PAA) molecules with Ca2+ ions in Mg-ACP clusters (similar to 2.1 +/- 0.5 nm) using a biomineralization strategy produced inorganic-organic Mg-ACP/PAA hybrid nanoparticles with better thermal stability. Mg-ACP/PAA hybrid nanoparticles (similar to 24.0 +/- 4.8 nm) were pH-responsive and could be efficiently digested under weak acidic conditions (pH 5.0-5.5). The internalization of assembled Mg-ACP/PAA nanoparticles by MC3T3-E1 cells occurred through endocytosis, indicated by laser scanning confocal microscopy and cryo-soft X-ray tomography. Our results showed that cellular lipid membranes remained intact without pore formation after Mg-ACP/PAA particle penetration. The assembled Mg-ACP/PAA particles could be digested in cell lysosomes within 24 h under weak acidic conditions, thereby indicating the potential to efficiently deliver encapsulated functional molecules. Both the in vitro and in vivo results preliminarily demonstrated good biosafety of the inorganic-organic Mg-ACP/PAA hybrid nanoparticles, which may have potential for biomedical applications.
关键词	Inorganic-organic hybrid pH responsive ACP clusters Cellular degradation Cryo-soft X-ray tomography
资助者	National Key Research and Development Program of China from Ministry of Science and Technology ; Key Research Program of Frontier Sciences from Chinese Academy of Sciences (CAS) ; Key Research and Development Program of Liaoning Province ; Shenyang Key R&D and Technology Transfer Program
DOI	10.1016/j.bioactmat.2021.01.005
收录类别	SCI
语种	英语
资助项目	National Key Research and Development Program of China from Ministry of Science and Technology[2016YFC1100502] ; Key Research Program of Frontier Sciences from Chinese Academy of Sciences (CAS)[QYZDY-SSW-JSC031] ; Key Research and Development Program of Liaoning Province[201703031] ; Shenyang Key R&D and Technology Transfer Program[Z17-7-023]
WOS研究方向	Engineering ; Materials Science
WOS类目	Engineering, Biomedical ; Materials Science, Biomaterials
WOS记录号	WOS:000648343800007
出版者	KEAI PUBLISHING LTD
引用统计	被引频次：26[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.imr.ac.cn/handle/321006/160761
专题	中国科学院金属研究所
通讯作者	Zhang, Xing
作者单位	1.Chinese Acad Sci, Inst Met Res, Shenyang 110016, Liaoning, Peoples R China 2.Univ Sci & Technol China, Sch Mat Sci & Engn, Hefei 230026, Anhui, Peoples R China 3.Gen Hosp Northern Theater Command, Dept Emergency Med, Shenyang 110016, Liaoning, Peoples R China 4.Univ Sci & Technol China, Natl Synchrotron Radiat Lab, Hefei 230029, Anhui, Peoples R China 5.Univ Queensland, Australian Inst Bioengn & Nanotechnol, Brisbane, Qld 4072, Australia
推荐引用方式 GB/T 7714	Li, Na,Cui, Wei,Cong, Peifang,et al. Biomimetic inorganic-organic hybrid nanoparticles from magnesium-substituted amorphous calcium phosphate clusters and polyacrylic acid molecules[J]. BIOACTIVE MATERIALS,2021,6(8):2303-2314.
APA	Li, Na.,Cui, Wei.,Cong, Peifang.,Tang, Jie.,Guan, Yong.,...&Zhang, Xing.(2021).Biomimetic inorganic-organic hybrid nanoparticles from magnesium-substituted amorphous calcium phosphate clusters and polyacrylic acid molecules.BIOACTIVE MATERIALS,6(8),2303-2314.
MLA	Li, Na,et al."Biomimetic inorganic-organic hybrid nanoparticles from magnesium-substituted amorphous calcium phosphate clusters and polyacrylic acid molecules".BIOACTIVE MATERIALS 6.8(2021):2303-2314.