Gradient nanostructured titanium stimulates cell responses in vitro and enhances osseointegration in vivo

doi:10.21037/atm-20-7588

IMR OpenIR

	Gradient nanostructured titanium stimulates cell responses in vitro and enhances osseointegration in vivo
	Cao, Nan-Jue 1,2; Zhu, Yu-He 1; Gao, Fei 1; Liang, Chen 3; Wang, Zhen-Bo 3; Zhang, Yue 1; Hao, Chun-Ping 1; Wang, Wei 1
通讯作者	Wang, Wei(wwang75@cmu.edu.cn)
	2021-04-01
发表期刊	ANNALS OF TRANSLATIONAL MEDICINE
ISSN	2305-5839
卷号	9 期号:7 页码:15
摘要	Background: Though titanium (Ti) is widely used as dental materials in the clinic, effective methods to treat Ti for higher surface biological activity still lack. Through Surface mechanical attrition treatment (SMAT) technology we could endow Ti with gradient nanostructured surface (GNS Ti). To investigate the biocompatibility of GNS Ti for its further application in dental implant field, we study the effects of GNS Ti on cell responses in vitro and osseointegration of the implant with surrounding bone tissues in vivo. Methods: In this study, GNS Ti was fabricated by SMAT. In vitro experiment, we co-cultured GNS Ti with bone mesenchymal stem cells (BMSCs), surface characterization was detected by transmission electron microscope (TEM). Adhesion, proliferation and differentiation of BMSCs were evaluated by scanning electron microscope (SEM), MTT, flow cytometry (FCM), alkaline phosphatase (ALP) and osteocalcin (OCN) tests. in vivo experiment, the GNS Ti was implanted into the rabbit mandible. Osteogenesis and osseointegration were evaluated by Micro CT, toluidine blue staining, and immunohistochemical staining at 4, 8, and 12 weeks postoperatively. Results: Both results showed that compared with the coarse grained (CG) Ti, the GNS Ti stimulated the adhesion, proliferation, and differentiation of BMSCs and improved osteogenesis and osseointegration. Conclusions: This study indicates that gradient nanostructured Ti is a promising material for dental implant application.
关键词	Surface mechanical attrition treatment (SMAT) titanium (Ti) nanostructure bone mesenchymal stem cells (BMSCs) osseointegration
资助者	National Natural Science Foundation of China ; Liaoning Provincial Key Research Plan Guidance Project ; Liaoning Provincial Natural Science Foundation Guidance Project ; Central Government of Liaoning Province to Guide Local Science and Technology Development Project ; Shenyang Major Scientific and Technological Innovation Research and Development Plan ; Shenyang Young and Middleaged Technological Innovation Talent Plan
DOI	10.21037/atm-20-7588
收录类别	SCI
语种	英语
资助项目	National Natural Science Foundation of China[81970980] ; Liaoning Provincial Key Research Plan Guidance Project[2018225078] ; Liaoning Provincial Natural Science Foundation Guidance Project[2019ZD0749] ; Central Government of Liaoning Province to Guide Local Science and Technology Development Project[2017108001] ; Shenyang Major Scientific and Technological Innovation Research and Development Plan[191124027] ; Shenyang Young and Middleaged Technological Innovation Talent Plan[RC200060]
WOS研究方向	Oncology ; Research & Experimental Medicine
WOS类目	Oncology ; Medicine, Research & Experimental
WOS记录号	WOS:000641416400001
出版者	AME PUBL CO
引用统计	被引频次：4[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.imr.ac.cn/handle/321006/161650
专题	中国科学院金属研究所
通讯作者	Wang, Wei
作者单位	1.China Med Univ, Sch & Hosp Stomatol, Liaoning Prov Key Lab Oral Dis, 117 Nanjing North St, Shenyang 110002, Peoples R China 2.Zhejiang Univ, Affiliated Hosp 4, Sch Med, Yiwu, Peoples R China 3.Chinese Acad Sci, Inst Met Res, Shenyang Natl Lab Mat Sci, Shenyang, Peoples R China
推荐引用方式 GB/T 7714	Cao, Nan-Jue,Zhu, Yu-He,Gao, Fei,et al. Gradient nanostructured titanium stimulates cell responses in vitro and enhances osseointegration in vivo[J]. ANNALS OF TRANSLATIONAL MEDICINE,2021,9(7):15.
APA	Cao, Nan-Jue.,Zhu, Yu-He.,Gao, Fei.,Liang, Chen.,Wang, Zhen-Bo.,...&Wang, Wei.(2021).Gradient nanostructured titanium stimulates cell responses in vitro and enhances osseointegration in vivo.ANNALS OF TRANSLATIONAL MEDICINE,9(7),15.
MLA	Cao, Nan-Jue,et al."Gradient nanostructured titanium stimulates cell responses in vitro and enhances osseointegration in vivo".ANNALS OF TRANSLATIONAL MEDICINE 9.7(2021):15.