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Gradient nanostructured titanium stimulates cell responses in vitro and enhances osseointegration in vivo
Cao, Nan-Jue1,2; Zhu, Yu-He1; Gao, Fei1; Liang, Chen3; Wang, Zhen-Bo3; Zhang, Yue1; Hao, Chun-Ping1; Wang, Wei1
Corresponding AuthorWang, Wei(wwang75@cmu.edu.cn)
2021-04-01
Source PublicationANNALS OF TRANSLATIONAL MEDICINE
ISSN2305-5839
Volume9Issue:7Pages:15
AbstractBackground: Though titanium (Ti) is widely used as dental materials in the clinic, effective methods to treat Ti for higher surface biological activity still lack. Through Surface mechanical attrition treatment (SMAT) technology we could endow Ti with gradient nanostructured surface (GNS Ti). To investigate the biocompatibility of GNS Ti for its further application in dental implant field, we study the effects of GNS Ti on cell responses in vitro and osseointegration of the implant with surrounding bone tissues in vivo. Methods: In this study, GNS Ti was fabricated by SMAT. In vitro experiment, we co-cultured GNS Ti with bone mesenchymal stem cells (BMSCs), surface characterization was detected by transmission electron microscope (TEM). Adhesion, proliferation and differentiation of BMSCs were evaluated by scanning electron microscope (SEM), MTT, flow cytometry (FCM), alkaline phosphatase (ALP) and osteocalcin (OCN) tests. in vivo experiment, the GNS Ti was implanted into the rabbit mandible. Osteogenesis and osseointegration were evaluated by Micro CT, toluidine blue staining, and immunohistochemical staining at 4, 8, and 12 weeks postoperatively. Results: Both results showed that compared with the coarse grained (CG) Ti, the GNS Ti stimulated the adhesion, proliferation, and differentiation of BMSCs and improved osteogenesis and osseointegration. Conclusions: This study indicates that gradient nanostructured Ti is a promising material for dental implant application.
KeywordSurface mechanical attrition treatment (SMAT) titanium (Ti) nanostructure bone mesenchymal stem cells (BMSCs) osseointegration
Funding OrganizationNational Natural Science Foundation of China ; Liaoning Provincial Key Research Plan Guidance Project ; Liaoning Provincial Natural Science Foundation Guidance Project ; Central Government of Liaoning Province to Guide Local Science and Technology Development Project ; Shenyang Major Scientific and Technological Innovation Research and Development Plan ; Shenyang Young and Middleaged Technological Innovation Talent Plan
DOI10.21037/atm-20-7588
Indexed BySCI
Language英语
Funding ProjectNational Natural Science Foundation of China[81970980] ; Liaoning Provincial Key Research Plan Guidance Project[2018225078] ; Liaoning Provincial Natural Science Foundation Guidance Project[2019ZD0749] ; Central Government of Liaoning Province to Guide Local Science and Technology Development Project[2017108001] ; Shenyang Major Scientific and Technological Innovation Research and Development Plan[191124027] ; Shenyang Young and Middleaged Technological Innovation Talent Plan[RC200060]
WOS Research AreaOncology ; Research & Experimental Medicine
WOS SubjectOncology ; Medicine, Research & Experimental
WOS IDWOS:000641416400001
PublisherAME PUBL CO
Citation statistics
Document Type期刊论文
Identifierhttp://ir.imr.ac.cn/handle/321006/161650
Collection中国科学院金属研究所
Corresponding AuthorWang, Wei
Affiliation1.China Med Univ, Sch & Hosp Stomatol, Liaoning Prov Key Lab Oral Dis, 117 Nanjing North St, Shenyang 110002, Peoples R China
2.Zhejiang Univ, Affiliated Hosp 4, Sch Med, Yiwu, Peoples R China
3.Chinese Acad Sci, Inst Met Res, Shenyang Natl Lab Mat Sci, Shenyang, Peoples R China
Recommended Citation
GB/T 7714
Cao, Nan-Jue,Zhu, Yu-He,Gao, Fei,et al. Gradient nanostructured titanium stimulates cell responses in vitro and enhances osseointegration in vivo[J]. ANNALS OF TRANSLATIONAL MEDICINE,2021,9(7):15.
APA Cao, Nan-Jue.,Zhu, Yu-He.,Gao, Fei.,Liang, Chen.,Wang, Zhen-Bo.,...&Wang, Wei.(2021).Gradient nanostructured titanium stimulates cell responses in vitro and enhances osseointegration in vivo.ANNALS OF TRANSLATIONAL MEDICINE,9(7),15.
MLA Cao, Nan-Jue,et al."Gradient nanostructured titanium stimulates cell responses in vitro and enhances osseointegration in vivo".ANNALS OF TRANSLATIONAL MEDICINE 9.7(2021):15.
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