Large-pore-size Ti6Al4V scaffolds with different pore structures for vascularized bone regeneration

doi:10.1016/j.msec.2021.112499

IMR OpenIR

	Large-pore-size Ti6Al4V scaffolds with different pore structures for vascularized bone regeneration
	Wang, Chao 1,2; Xu, Duoling 1,2; Lin, Ling 1,2; Li, Shujun 3; Hou, Wentao 3; He, Yi 1,2; Sheng, Liyuan 4; Yi, Chen 1,2; Zhang, Xiliu 1,2; Li, Hongyu 1,2; Li, Yiming 1,2; Zhao, Wei 1,2; Yu, Dongsheng 1,2
通讯作者	Zhao, Wei(zhaowei3@mail.sysu.edu.cn) ; Yu, Dongsheng(yudsh@mail.sysu.edu.cn)
	2021-12-01
发表期刊	MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS
ISSN	0928-4931
卷号	131 页码:12
摘要	Porous Ti6Al4V scaffolds are characterized by high porosity, low elastic modulus, and good osteogenesis and vascularization, which are expected to facilitate the repair of large-scale bone defects in future clinical appli-cations. Ti6Al4V scaffolds are divided into regular and irregular structures according to the pore structure, but the pore structure more capable of promoting bone regeneration and angiogenesis has not yet been reported. The purpose of this study was to explore the optimal pore structure and pore size of the Ti6Al4V porous scaffold for the repair of large-area bone defects and the promotion of vascularization in the early stage of osteogenesis. 7 groups of porous Ti6Al4V scaffolds, named NP, R8, R9, R10, P8, P9 and P10, were fabricated by Electron-beam-melting (EBM). Live/dead staining, immunofluorescence staining, SEM, CCK8, ALP, and PCR were used to detect the adhesion, proliferation, and differentiation of BMSCs on different groups of scaffolds. Hematoxylin-eosin (HE) staining and Van Gieson (VG) staining were used to detect bone regeneration and angiogenesis in vivo. The research results showed that as the pore size of the scaffold increased, the surface area and volume of the scaffold gradually decreased, and cell proliferation ability and cell viability gradually increased. The ability of cells to vascularize on scaffolds with irregular pore sizes was stronger than that on scaffolds with regular pore sizes. Micro-CT 3D reconstruction images showed that bone regeneration was obvious and new blood vessels were thick on the P10 scaffold. HE and VG staining showed that the proportion of bone area on the scaffolds with irregular pores was higher than that on scaffolds with regular pores. P10 had better mechanical properties and were more conducive to bone tissue ingrowth and blood vessel formation, thereby facilitating the repair of large -area bone defects.
关键词	Irregular porous structures Pore size Large-area bone defect Vascularization
DOI	10.1016/j.msec.2021.112499
收录类别	SCI
语种	英语
WOS研究方向	Materials Science
WOS类目	Materials Science, Biomaterials
WOS记录号	WOS:000712075300002
出版者	ELSEVIER
引用统计	被引频次：72[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.imr.ac.cn/handle/321006/167168
专题	中国科学院金属研究所
通讯作者	Zhao, Wei; Yu, Dongsheng
作者单位	1.Sun Yat Sen Univ, Guanghua Sch Stomatol, Hosp Stomatol, Guangzhou 510055, Peoples R China 2.Sun Yat Sen Univ, Guangdong Prov Key Lab Stomatol, Guangzhou 510050, Peoples R China 3.Chinese Acad Sci, Inst Met Res, Shenyang 110016, Peoples R China 4.Peking Univ, Shenzhen Inst, Shenzhen 518057, Peoples R China
推荐引用方式 GB/T 7714	Wang, Chao,Xu, Duoling,Lin, Ling,et al. Large-pore-size Ti6Al4V scaffolds with different pore structures for vascularized bone regeneration[J]. MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS,2021,131:12.
APA	Wang, Chao.,Xu, Duoling.,Lin, Ling.,Li, Shujun.,Hou, Wentao.,...&Yu, Dongsheng.(2021).Large-pore-size Ti6Al4V scaffolds with different pore structures for vascularized bone regeneration.MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS,131,12.
MLA	Wang, Chao,et al."Large-pore-size Ti6Al4V scaffolds with different pore structures for vascularized bone regeneration".MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS 131(2021):12.