Three-Dimensionally Printed Ti2448 With Low Stiffness Enhanced Angiogenesis and Osteogenesis by Regulating Macrophage Polarization via Piezo1/YAP Signaling Axis

doi:10.3389/fcell.2021.750948

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	Three-Dimensionally Printed Ti2448 With Low Stiffness Enhanced Angiogenesis and Osteogenesis by Regulating Macrophage Polarization via Piezo1/YAP Signaling Axis
	Tang, Zhen 1; Wei, Xinghui 1; Li, Tian 2; Wu, Hao 1; Xiao, Xin 1; Hao, Yulin 3; Li, Shujun 3; Hou, Wentao 3; Shi, Lei 1; Li, Xiaokang 4; Guo, Zheng 4
通讯作者	Shi, Lei(shilei_med@163.com) ; Li, Xiaokang(lxkfmmu@163.com) ; Guo, Zheng(guozheng@fmmu.edu.cn)
	2021-11-15
发表期刊	FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
ISSN	2296-634X
卷号	9 页码:20
摘要	Previous studies have found that the novel low-elastic-modulus Ti2448 alloy can significantly reduce stress shielding and contribute to better bone repair than the conventional Ti6Al4V alloy. In this study, the promotion of osteogenesis and angiogenesis by three-dimensionally printed Ti2448 were also observed in vivo. However, these were not significant in a series of in vitro tests. The stiffness of materials has been reported to greatly affect the response of macrophages, and the immunological regulation mediated by macrophages directly determines the fate of bone implants. Therefore, we designed more experiments to explore the role of three-dimensionally printed Ti2448 in macrophage activation and related osteogenesis and angiogenesis. As expected, we found a significant increase in the number of M2 macrophages around Ti2448 implants, as well as better osteogenesis and angiogenesis in vivo. In vitro studies also showed that macrophages pre-treated with Ti2448 alloy significantly promoted angiogenesis and osteogenic differentiation through increased PDGF-BB and BMP-2 secretion, and the polarization of M2 macrophages was enhanced. We deduced that Ti2448 promotes angiogenesis and osteogenesis through Piezo1/YAP signaling axis-mediated macrophage polarization and related cytokine secretion. This research might provide insight into the biological properties of Ti2448 and provide a powerful theoretical supplement for the future application of three-dimensionally printed Ti2448 implants in orthopaedic surgery.
关键词	Ti2448 macrophage polarization angiogenesis osteogenesis
DOI	10.3389/fcell.2021.750948
收录类别	SCI
语种	英语
WOS研究方向	Cell Biology ; Developmental Biology
WOS类目	Cell Biology ; Developmental Biology
WOS记录号	WOS:000725605000001
出版者	FRONTIERS MEDIA SA
引用统计	被引频次：40[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.imr.ac.cn/handle/321006/167578
专题	中国科学院金属研究所
通讯作者	Shi, Lei; Li, Xiaokang; Guo, Zheng
作者单位	1.Fourth Mil Med Univ, Xijing Hosp, Dept Orthopaed, Xian, Peoples R China 2.Fourth Mil Med Univ, Sch Basic Med, Xian, Peoples R China 3.Chinese Acad Sci, Inst Met Res, Shenyang, Peoples R China 4.Fourth Mil Med Univ, Tangdu Hosp, Dept Orthopaed, Xian, Peoples R China
推荐引用方式 GB/T 7714	Tang, Zhen,Wei, Xinghui,Li, Tian,et al. Three-Dimensionally Printed Ti2448 With Low Stiffness Enhanced Angiogenesis and Osteogenesis by Regulating Macrophage Polarization via Piezo1/YAP Signaling Axis[J]. FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY,2021,9:20.
APA	Tang, Zhen.,Wei, Xinghui.,Li, Tian.,Wu, Hao.,Xiao, Xin.,...&Guo, Zheng.(2021).Three-Dimensionally Printed Ti2448 With Low Stiffness Enhanced Angiogenesis and Osteogenesis by Regulating Macrophage Polarization via Piezo1/YAP Signaling Axis.FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY,9,20.
MLA	Tang, Zhen,et al."Three-Dimensionally Printed Ti2448 With Low Stiffness Enhanced Angiogenesis and Osteogenesis by Regulating Macrophage Polarization via Piezo1/YAP Signaling Axis".FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY 9(2021):20.