Antibacterial and anti-inflammatory activities of chitosan/copper complex coating on medical catheters: In vitro and in vivo

doi:10.1002/jbm.b.35047

IMR OpenIR

	Antibacterial and anti-inflammatory activities of chitosan/copper complex coating on medical catheters: In vitro and in vivo
	Gu, Guisong 1,2; Erisen, Deniz Eren 1,3; Yang, Ke 1; Zhang, Bingchun 1; Shen, Minggang 2; Zou, Jingyu 4; Qi, Xun 5; Chen, Shanshan 1; Xu, Xiaohe 6
通讯作者	Chen, Shanshan(sschen@imr.ac.cn) ; Xu, Xiaohe(32626292@qq.com)
	2022-03-07
发表期刊	JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS
ISSN	1552-4973
页码	12
摘要	Copper ions (Cu) grafted chitosan coating was prepared using the pneumatic spraying method on the silicone rubber surface. Coating's surface properties, morphology, composition, Cu releasing behavior, antibacterial, and anti-inflammatory activities are investigated and discussed. Surface properties, composition, and morphology were investigated by scanning electron microscopy (SEM) and contact angle measurements. The antibacterial activity has been tested with Escherichia coli and Staphylococcus aureus suspensions in vitro. Besides, the morphology of the biofilm was inspected with a field emission SEM. To evaluate the anti-inflammatory activity and biosafety of the coating in vivo, the optimized coating samples and control groups were implanted subcutaneously into the back of mice. The bacterial environment model was established by injection of the bacterial suspension. The morphology and bacterial adhered on the surface of catheters and the surrounding tissues were analyzed after 5 days of implantation. As in vitro results, the number of adhered bacterial on the surface of the silicon rubber surface was decreased, and the anti-inflammatory rate was increased by the intensify of the Cu content in chitosan coating. As for in vivo results, after 5 days of implantation, there was no evident inflammation in the surrounding tissues of all catheters in all without the S. aureus injected group. In the injected chitosan/Cu coated group; the inflammation, the number of the adhered bacteria were observed less than other injected samples without Cu; no inflammation were noticeable. Results indicate that the Cu-modified chitosan coating can confer excellent antibacterial and anti-inflammatory activity as applied on medical catheters.
关键词	antibacterial catheter chitosan copper ion inflammation
资助者	Doctoral Start-up Foundation of Liaoning Province ; National Key Research and Development Program of China ; National Natural Science Foundation of China ; Youth Innovation Promotion Association of the Chinese Academy of Sciences
DOI	10.1002/jbm.b.35047
收录类别	SCI
语种	英语
资助项目	Doctoral Start-up Foundation of Liaoning Province[2019-BS-293] ; National Key Research and Development Program of China[2018YFC1105504] ; National Natural Science Foundation of China[51901227] ; National Natural Science Foundation of China[81701048] ; National Natural Science Foundation of China[81873918] ; Youth Innovation Promotion Association of the Chinese Academy of Sciences[2019194]
WOS研究方向	Engineering ; Materials Science
WOS类目	Engineering, Biomedical ; Materials Science, Biomaterials
WOS记录号	WOS:000765366900001
出版者	WILEY
引用统计	被引频次：18[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.imr.ac.cn/handle/321006/173241
专题	中国科学院金属研究所
通讯作者	Chen, Shanshan; Xu, Xiaohe
作者单位	1.Chinese Acad Sci, Shi Changxu Innovat Ctr Adv Mat, Inst Met Res, 72 Wenhua Rd, Shenyang 110016, Liaoning, Peoples R China 2.Univ Sci & Technol Liaoning, Sch Mat & Met, Anshan, Peoples R China 3.Univ Sci & Technol China, Sch Mat Sci & Engn, Hefei, Anhui, Peoples R China 4.China Med Univ, Dept Neurosurg, Affiliated Hosp 1, Shenyang, Peoples R China 5.China Med Univ, Dept Radiol, Affiliated Hosp 1, Key Lab Diagnost Imaging & Intervent Radiol Liaon, Shenyang, Peoples R China 6.China Med Univ, Dept Ophthalmol, Shengjing Hosp, 36 Sanhao St, Shenyang 110001, Peoples R China
推荐引用方式 GB/T 7714	Gu, Guisong,Erisen, Deniz Eren,Yang, Ke,et al. Antibacterial and anti-inflammatory activities of chitosan/copper complex coating on medical catheters: In vitro and in vivo[J]. JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS,2022:12.
APA	Gu, Guisong.,Erisen, Deniz Eren.,Yang, Ke.,Zhang, Bingchun.,Shen, Minggang.,...&Xu, Xiaohe.(2022).Antibacterial and anti-inflammatory activities of chitosan/copper complex coating on medical catheters: In vitro and in vivo.JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS,12.
MLA	Gu, Guisong,et al."Antibacterial and anti-inflammatory activities of chitosan/copper complex coating on medical catheters: In vitro and in vivo".JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS (2022):12.