IMR OpenIR
Inhibition efficiency of 304-Cu stainless steel against oral bacterial biofilm
Lan, Yiliang1; Yang, Jie1; Liu, Xianbo1; Zhao, Hanyu2; Zhang, Xinrui2; Yin, Xueliang3; Yang, Chunguang2; Yang, Ke2; Liu, Yi1
Corresponding AuthorYang, Chunguang(cgyang@imr.ac.cn) ; Liu, Yi(liuyi@cmu.edu.cn)
2022
Source PublicationJOURNAL OF APPLIED BIOMATERIALS & FUNCTIONAL MATERIALS
Volume20Pages:11
AbstractPurpose: This study aims to evaluate the antibacterial properties of 304 Cu-bearing stainless steel (SS) with different Cu contents (0, 2.5, 4.5 wt.%) against oral biofilms of Streptococcus mutans (S. mutans), Streptococcus sanguinis (S. sanguinis), and their mixture. Methods: Bacterial biofilms on the surface of 304-Cu SS were characterized by plate counting, 4 ', 6-diamidino-2-phenylindole (DAPI) staining with aid of sanning electron microscopy (SEM) and 2, 3-bis (2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide inner salt (XTT). In addition, the inhibition zone method was also employed to evaluate the antibacterial properties of 304-Cu SS. Cell Counting Kit-8 (CCK-8) and flow cytometry were used to assess the cytotoxicity and apoptosis rate of 304-Cu SS, respectively. Results: 304-4.5Cu SS could effectively inhibit the attachment, formation, activity, and metabolism of bacterial biofilm, possessing the best antibacterial properties exceeding 99.9% of antibacterial rate against S. mutans, S. sanguinis, and their mixture. The diameters of inhibition zones to S. mutans and S. sanguinis on the surface of 304-4.5Cu SS were 21.7 and 14.7 mm, respectively. The results of cell experiments in vitro showed that both 304-2.5Cu SS and 304-4.5Cu SS had no evident cytotoxicity with an identical grade 1. The apoptosis rate exhibited a gradually increased tendency with increase of the Cu content in 304 SS. Conclusions: 304-4.5Cu SS without cytotoxic effect on NIH3T3 cells has obvious antibacterial activity against S. mutans, S. sanguinis and their mixture. Clinical significance: The Cu-bearing stainless steel provides a new solution to be used as oral orthodontic devices for inhibiting oral microflora imbalance and enamel demineralization.
KeywordCopper-bearing stainless steel S mutans S sanguinis cytotoxicity corrosion resistance
Funding OrganizationNational Key Research and Development Program of China ; National Natural Science Foundation of China ; Liaoning Province Key Research and Development Plan Project ; Foundation of Liaoning Educational Committee of China ; Youth innovation promotion association CAS ; Shi-changxu Innovation Center for Advanced Materials, Institute of Metal Research, Chinese Academy of Sciences
DOI10.1177/22808000211065259
Indexed BySCI
Language英语
Funding ProjectNational Key Research and Development Program of China[2016YFB0300205] ; National Natural Science Foundation of China[51771199] ; National Natural Science Foundation of China[51631009] ; Liaoning Province Key Research and Development Plan Project[2020JH2/10300038] ; Foundation of Liaoning Educational Committee of China[L2019lkyjc-03] ; Youth innovation promotion association CAS[2018221] ; Shi-changxu Innovation Center for Advanced Materials, Institute of Metal Research, Chinese Academy of Sciences
WOS Research AreaBiophysics ; Engineering ; Materials Science
WOS SubjectBiophysics ; Engineering, Biomedical ; Materials Science, Biomaterials
WOS IDWOS:000752637900001
PublisherSAGE PUBLICATIONS LTD
Citation statistics
Document Type期刊论文
Identifierhttp://ir.imr.ac.cn/handle/321006/173497
Collection中国科学院金属研究所
Corresponding AuthorYang, Chunguang; Liu, Yi
Affiliation1.China Med Univ, Sch & Hosp Stomatol, Liaoning Prov Key Lab Oral Dis, 117 Nanjing North St, Shenyang 110002, Peoples R China
2.Chinese Acad Sci, Inst Met Res, 72 Wenhua Rd, Shenyang 110016, Liaoning, Peoples R China
3.Liaoning Inst Sci & Technol, Sch Met, Benxi, Peoples R China
Recommended Citation
GB/T 7714
Lan, Yiliang,Yang, Jie,Liu, Xianbo,et al. Inhibition efficiency of 304-Cu stainless steel against oral bacterial biofilm[J]. JOURNAL OF APPLIED BIOMATERIALS & FUNCTIONAL MATERIALS,2022,20:11.
APA Lan, Yiliang.,Yang, Jie.,Liu, Xianbo.,Zhao, Hanyu.,Zhang, Xinrui.,...&Liu, Yi.(2022).Inhibition efficiency of 304-Cu stainless steel against oral bacterial biofilm.JOURNAL OF APPLIED BIOMATERIALS & FUNCTIONAL MATERIALS,20,11.
MLA Lan, Yiliang,et al."Inhibition efficiency of 304-Cu stainless steel against oral bacterial biofilm".JOURNAL OF APPLIED BIOMATERIALS & FUNCTIONAL MATERIALS 20(2022):11.
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