| Electrochemically derived nanographene oxide activates endothelial tip cells and promotes angiogenesis by binding endogenous lysophosphatidic acid | |
| Liu, Wenjing1,2; Luo, Haiyun1; Wei, Qinwei3; Liu, Jia1; Wu, Junrong1; Zhang, Yanli1; Chen, Lili4; Ren, Wencai3; Shao, Longquan1,2 | |
| Corresponding Author | Shao, Longquan(shaolongquan@smu.edu.cn) |
| 2022-03-01 | |
| Source Publication | BIOACTIVE MATERIALS
 |
| Volume | 9Pages:92-104 |
| Abstract | Graphene oxide (GO) exhibits good mechanical and physicochemical characteristics and has extensive application prospects in bone tissue engineering. However, its effect on angiogenesis is unclear, and its potential toxic effects are heavily disputed. Herein, we found that nanographene oxide (NGO) synthesized by one-step water electrolytic oxidation is smaller and shows superior biocompatibility. Moreover, NGO significantly enhanced angiogenesis in calvarial bone defect areas in vivo, providing a good microenvironment for bone regeneration. Endothelial tip cell differentiation is an important step in the initiation of angiogenesis. We verified that NGO activates endothelial tip cells by coupling with lysophosphatidic acid (LPA) in serum via strong hydrogen bonding interactions, which has not been reported. In addition, the mechanism by which NGO promotes angiogenesis was systematically studied. NGO-coupled LPA activates LPAR6 and facilitates the formation of migratory tip cells via Hippo/Yes-associated protein (YAP) independent of reactive oxygen species (ROS) stimulation or additional complex modifications. These results provide an effective strategy for the application of electrochemically derived NGO and more insight into NGO-mediated angiogenesis. |
| Keyword | Nanographene oxide Lysophosphatidic acid Hydrogen bonding Endothelial tip cell Hippo signalling |
| Funding Organization | National Natural Science Foundation of China ; Natural Science Foundation of Guangdong Province ; Guangdong Basic and Applied Basic Research Foundation |
| DOI | 10.1016/j.bioactmat.2021.07.007 |
| Indexed By | SCI |
| Language | 英语 |
| Funding Project | National Natural Science Foundation of China[52072167] ; National Natural Science Foundation of China[81900989] ; Natural Science Foundation of Guangdong Province[2019A1515011980] ; Guangdong Basic and Applied Basic Research Foundation[2019A1515110088] |
| WOS Research Area | Engineering ; Materials Science |
| WOS Subject | Engineering, Biomedical ; Materials Science, Biomaterials |
| WOS ID | WOS:000736276600001 |
| Publisher | KEAI PUBLISHING LTD |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.imr.ac.cn/handle/321006/173816 |
| Collection | 中国科学院金属研究所 |
| Corresponding Author | Shao, Longquan |
| Affiliation | 1.Southern Med Univ, Stomatol Hosp, Guangzhou 510280, Peoples R China 2.Guangdong Prov Key Lab Construct & Detect Tissue, Guangzhou 510515, Peoples R China 3.Chinese Acad Sci, Inst Met Res, Shenyang Natl Lab Mat Sci, Shenyang 110016, Peoples R China 4.Huazhong Univ Sci & Technol, Union Hosp, Tongji Med Coll, Stomatol, Wuhan 430022, Peoples R China |
| Recommended Citation GB/T 7714 | Liu, Wenjing,Luo, Haiyun,Wei, Qinwei,et al. Electrochemically derived nanographene oxide activates endothelial tip cells and promotes angiogenesis by binding endogenous lysophosphatidic acid[J]. BIOACTIVE MATERIALS,2022,9:92-104. |
| APA | Liu, Wenjing.,Luo, Haiyun.,Wei, Qinwei.,Liu, Jia.,Wu, Junrong.,...&Shao, Longquan.(2022).Electrochemically derived nanographene oxide activates endothelial tip cells and promotes angiogenesis by binding endogenous lysophosphatidic acid.BIOACTIVE MATERIALS,9,92-104. |
| MLA | Liu, Wenjing,et al."Electrochemically derived nanographene oxide activates endothelial tip cells and promotes angiogenesis by binding endogenous lysophosphatidic acid".BIOACTIVE MATERIALS 9(2022):92-104. |
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