Size-dependent interactions between calciprotein particles and vascular endothelium

doi:10.1016/j.mtbio.2025.101599

IMR OpenIR

	Size-dependent interactions between calciprotein particles and vascular endothelium
	Zhang, Zeping 1,2; Wang, Xinyue 1,2; Huang, Caihao 1,3; Wang, Meixia 4; Cui, Wei 1,2; Hao, Liang 5; Yang, Rui 1,2; Wang, Hong-hui 4; Zhang, Xing 1,2
通讯作者	Wang, Hong-hui(wanghonghui@hnu.edu.cn) ; Zhang, Xing(xingzhang@imr.ac.cn)
	2025-04-01
发表期刊	MATERIALS TODAY BIO
ISSN	2590-0064
卷号	31 页码:12
摘要	The underlying mechanisms governing the interactions between nanoparticles and vascular endothelial barrier remain largely unexplored, which is crucial for the optimal design of nanoparticles for clinical applications. In this study, the size-dependent interactions between calciprotein particles (CPPs) and endothelial cells (ECs) were investigated using a rat model of chronic kidney disease (CKD) induced by 5/6 nephrectomy. Two primary types of CPP1 were studied: small-sized CPP1 (S-CPP1, <50 nm) and larger CPP1 (L-CPP1, <100 nm), detected three and five weeks post-surgery, respectively. By adjusting the amounts of Ca2+, HPO42- and H2PO4- ions in Dulbecco's Modified Eagle Medium supplemented with 10 % (V/V) fetal bovine serum and 1 % (V/V) Pen-Strep solution, S-CPP1 (<50 nm) with an elliptical shape, L-CPP1 (50-100 nm), and secondary CPPs (CPP2, >100 nm) with a needle-like crystalline structure, resembling endogenous CPPs, were synthesized. The results showed that S-CPP1 significantly increased endothelial permeability at concentrations of 445 mu g/mL and 890 mu g/mL, thereby disrupting the integrity of the endothelial barrier formed by a confluent monolayer of ECs. Immunofluorescence analysis revealed that L-CPP1 was internalized by ECs via endocytosis, while S-CPP1 disrupted VE-cadherin junctions, leading to irregular cell morphology and widened intercellular gaps. These structural changes likely contribute to medial calcification as CPPs accumulate within the circulatory system. In conclusion, the findings underscore that the interaction between CPPs and the vascular endothelium is strongly size-dependent, with significant implications for vascular system health and the design of nanoparticle-based therapies.
关键词	Endothelium Calciprotein nanoparticles Chronic kidney disease Biomimetic synthesis
资助者	National Natural Science Foundation of China ; Liaoning Provincial Science and Technology Plan Project
DOI	10.1016/j.mtbio.2025.101599
收录类别	SCI
语种	英语
资助项目	National Natural Science Foundation of China[52273278] ; Liaoning Provincial Science and Technology Plan Project[2022-YGJC-32]
WOS研究方向	Engineering ; Materials Science
WOS类目	Engineering, Biomedical ; Materials Science, Biomaterials
WOS记录号	WOS:001436871000001
出版者	ELSEVIER
引用统计
文献类型	期刊论文
条目标识符	http://ir.imr.ac.cn/handle/321006/179736
专题	中国科学院金属研究所
通讯作者	Wang, Hong-hui; Zhang, Xing
作者单位	1.Chinese Acad Sci, Inst Met Res, Shenyang 110016, Liaoning, Peoples R China 2.Univ Sci & Technol China, Sch Mat Sci & Engn, Hefei 230026, Anhui, Peoples R China 3.Dalian Univ Technol, Sch Mat Sci & Engn, Dalian 116024, Liaoning, Peoples R China 4.Hunan Univ, Coll Biol, State Key Lab Chemo Biosensing & Chemometr, Changsha 410082, Hunan, Peoples R China 5.China Med Univ, Sch Forens Med, Shenyang 110026, Liaoning, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Zeping,Wang, Xinyue,Huang, Caihao,et al. Size-dependent interactions between calciprotein particles and vascular endothelium[J]. MATERIALS TODAY BIO,2025,31:12.
APA	Zhang, Zeping.,Wang, Xinyue.,Huang, Caihao.,Wang, Meixia.,Cui, Wei.,...&Zhang, Xing.(2025).Size-dependent interactions between calciprotein particles and vascular endothelium.MATERIALS TODAY BIO,31,12.
MLA	Zhang, Zeping,et al."Size-dependent interactions between calciprotein particles and vascular endothelium".MATERIALS TODAY BIO 31(2025):12.