Selenium Nanoparticles Suppressed Oxidative Stress and Promoted Tenocyte Marker Expression in Tendon-Derived Stem/Progenitor Cells

doi:10.3390/antiox13121536

IMR OpenIR

	Selenium Nanoparticles Suppressed Oxidative Stress and Promoted Tenocyte Marker Expression in Tendon-Derived Stem/Progenitor Cells
	Lui, Pauline Po Yee 1,2; Huang, Caihao 3,4; Zhang, Xing 3
通讯作者	Lui, Pauline Po Yee(paulinelui@cuhk.edu.hk)
	2024-12-01
发表期刊	ANTIOXIDANTS
卷号	13 期号:12 页码:20
摘要	Traumatic tendon injuries generate reactive oxygen species and inflammation, which may account for slow or poor healing outcomes. Selenium is an essential trace element presented in selenoproteins, many of which are strong antioxidant enzymes. Selenium nanoparticles (SeNPs) have been reported to promote tissue repair due to their anti-oxidative, anti-inflammatory, anti-apoptotic, and differentiation-modulating properties. However, its effects on the functions of tendon-derived stem/progenitor cells (TDSCs) and tendon healing have not been reported. This study examined the effects of SeNPs on the functions of hydroperoxide (H2O2)-stimulated TDSCs. Rat patellar TDSCs were treated with H2O2 with or without SeNPs. The viability, marker of proliferation, oxidative stress, inflammation, apoptosis, and tenocyte marker expressions of H2O2-stimulated TDSCs after SeNPs treatment were assessed. Our results showed that SeNPs increased the viability and expression of the marker of proliferation of TDSCs exposed to H2O2, while concurrently reducing oxidative stress, inflammation, and apoptosis. Additionally, the expressions of tenocyte markers were significantly elevated in H2O2-treated TDSCs after treatment with SeNPs. Furthermore, the expressions of Sirt1 and Nrf2 also increased after SeNPs treatment in H2O2-stimulated TDSCs. In conclusion, SeNPs mitigated oxidative stress, inflammation, and apoptosis while enhancing the survival and expression of the marker of proliferation of TDSCs in an oxidative stress environment. Additionally, it promoted the fate of TDSCs towards the tenocyte lineage in the presence of such oxidative stress. The increased expressions of Sirt1 and Nrf2 likely mediated the anti-oxidative and anti-inflammatory effects of SeNPs. SeNPs hold promise as a novel intervention for promoting tendon healing.
关键词	inflammation oxidative stress selenium tendon-derived stem/progenitor cells (TDSCs)
资助者	Innovation and Technology Commission, the Government of the Hong Kong Special Administrative Region of the People's Republic of China ; Center of Neuromusculoskeletal Restorative Medicine Ltd. ; Innovation and Technology Commission ; Government of the Hong Kong Special Administrative Region of the People's Republic of China ; Chinese University of Hong Kong
DOI	10.3390/antiox13121536
收录类别	SCI
语种	英语
资助项目	Innovation and Technology Commission, the Government of the Hong Kong Special Administrative Region of the People's Republic of China ; Center of Neuromusculoskeletal Restorative Medicine Ltd. ; Innovation and Technology Commission ; Government of the Hong Kong Special Administrative Region of the People's Republic of China[ITC RC/IHK/4/7] ; Chinese University of Hong Kong
WOS研究方向	Biochemistry & Molecular Biology ; Pharmacology & Pharmacy ; Food Science & Technology
WOS类目	Biochemistry & Molecular Biology ; Chemistry, Medicinal ; Food Science & Technology
WOS记录号	WOS:001384113700001
出版者	MDPI
引用统计	被引频次：1[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.imr.ac.cn/handle/321006/181193
专题	中国科学院金属研究所
通讯作者	Lui, Pauline Po Yee
作者单位	1.Chinese Univ Hong Kong, Dept Orthopaed & Traumatol, Shatin, Hong Kong, Peoples R China 2.Ctr Neuromusculoskeletal Restorat Med Ltd, Shatin, Hong Kong Sci Pk, Hong Kong, Peoples R China 3.Chinese Acad Sci, Inst Met Res, Shenyang 110000, Peoples R China 4.Dalian Univ Technol, Sch Mat Sci & Engn, Dalian 116000, Peoples R China
推荐引用方式 GB/T 7714	Lui, Pauline Po Yee,Huang, Caihao,Zhang, Xing. Selenium Nanoparticles Suppressed Oxidative Stress and Promoted Tenocyte Marker Expression in Tendon-Derived Stem/Progenitor Cells[J]. ANTIOXIDANTS,2024,13(12):20.
APA	Lui, Pauline Po Yee,Huang, Caihao,&Zhang, Xing.(2024).Selenium Nanoparticles Suppressed Oxidative Stress and Promoted Tenocyte Marker Expression in Tendon-Derived Stem/Progenitor Cells.ANTIOXIDANTS,13(12),20.
MLA	Lui, Pauline Po Yee,et al."Selenium Nanoparticles Suppressed Oxidative Stress and Promoted Tenocyte Marker Expression in Tendon-Derived Stem/Progenitor Cells".ANTIOXIDANTS 13.12(2024):20.