A Novel Fluorescent Aptasensor Based on Mesoporous Silica Nanoparticles for Selective and Sensitive Detection of Saxitoxin in Shellfish

doi:10.2174/0115734110269897231020065609

IMR OpenIR

	A Novel Fluorescent Aptasensor Based on Mesoporous Silica Nanoparticles for Selective and Sensitive Detection of Saxitoxin in Shellfish
	Ullah, Najeeb 1,2; Noureen, Beenish 3; Zahra, Qurat Ul Ain 4; Aziz, Tariq 5; Shehzadi, Somia 6; Alfaifif, Mohammad Y.7; Elbehairif, Serag Eldin I.7; Thebo, Khalid Hussain 8; Ullah, Asmat 9; Iqbal, Haroon 10
通讯作者	Ullah, Asmat(asmat_masood@yahoo.com) ; Iqbal, Haroon(harooniqbal415@hotmail.com)
	2023
发表期刊	CURRENT ANALYTICAL CHEMISTRY
ISSN	1573-4110
卷号	19 期号:9 页码:677-684
摘要	Background: Saxitoxin (STX) stands as one of the most potent marine biotoxins, exhibiting high lethality. Despite its severity, current treatments remain ineffective, and existing detection techniques are limited due to ethical concerns and technical constraints.Methods: Herein, an innovative approach was constructed for STX detection, utilizing mesoporous silica nanoparticles (MSN) as a foundation. This innovative, easy, and label-free aptamer (Apt)-sensor was fabricated. Apts were employed as molecular identification probes and "gated molecules," while rhodamine 6G was encapsulated within particles to serve as a signal probe. In a lack of STX, Apts immobilized on an MSN surface kept a "gate" closed, preventing signal probe leakage. Upon the presence of STX, the "gate" opened, allowing a particular binding of Apts to STX and a subsequent release of a signal probe.Results: Experimental results demonstrated a positive correlation between fluorescence intensity and concentrations of STX within a range of 1 to 80 nM, with an exceptional limit of detection of 0.12 nM. Furthermore, the selectivity and stability of a biosensor were rigorously evaluated, validating its reliability.Conclusion: This newly developed sensing strategy exhibits remarkable performance in STX detection. Its success holds significant promise for advancing portable STX detection equipment, thereby addressing a pressing need for efficient and ethical detection methods in combating marine biotoxin contamination.
关键词	Aptamer fluorescent sensing saxitoxin mesoporous silica nanoparticles(,) marine toxins shellfish poisoning
资助者	Deanship of Scientific Research at King Khalid University
DOI	10.2174/0115734110269897231020065609
收录类别	SCI
语种	英语
资助项目	Deanship of Scientific Research at King Khalid University[R.G.P. 2/ 59 /44]
WOS研究方向	Chemistry
WOS类目	Chemistry, Analytical
WOS记录号	WOS:001134359900003
出版者	BENTHAM SCIENCE PUBL LTD
引用统计	被引频次：7[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.imr.ac.cn/handle/321006/183236
专题	中国科学院金属研究所
通讯作者	Ullah, Asmat; Iqbal, Haroon
作者单位	1.Shaanxi Normal Univ, Sch Chem & Chem Engn, Key Lab Appl Surface & Colloid Chem, Xian 710119, Peoples R China 2.Univ Tennessee, Dept Chem Engn, Chattanooga, TN 37403 USA 3.Xi An Jiao Tong Univ, Inst Med Engn, Sch Basic Med Sci, Dept Biophys,Hlth Ctr, Xian 710061, Shaanxi, Peoples R China 4.Univ Sci & Technol China USTC, Biomed Imaging Ctr, Hefei, Peoples R China 5.Jiangsu Univ, Fac Civil Engn & Mech, Zhenjiang 212013, Peoples R China 6.Univ Lahore, Univ Inst Med Lab Technol, Lahore 54000, Pakistan 7.King Khalid Univ, Fac Sci, Dept Biol, Abha 9004, Saudi Arabia 8.Chinese Acad Sci, Inst Met Res, Shenyang, Peoples R China 9.Zhejiang Prov Peoples Hosp, Clin Res Inst, Hangzhou 310014, Zhejiang, Peoples R China 10.Chinese Acad Sci Hangzhou, Zhejiang Canc Hosp, Inst Basic Med & Canc IBMC, Hangzhou 310022, Zhejiang, Peoples R China
推荐引用方式 GB/T 7714	Ullah, Najeeb,Noureen, Beenish,Zahra, Qurat Ul Ain,et al. A Novel Fluorescent Aptasensor Based on Mesoporous Silica Nanoparticles for Selective and Sensitive Detection of Saxitoxin in Shellfish[J]. CURRENT ANALYTICAL CHEMISTRY,2023,19(9):677-684.
APA	Ullah, Najeeb.,Noureen, Beenish.,Zahra, Qurat Ul Ain.,Aziz, Tariq.,Shehzadi, Somia.,...&Iqbal, Haroon.(2023).A Novel Fluorescent Aptasensor Based on Mesoporous Silica Nanoparticles for Selective and Sensitive Detection of Saxitoxin in Shellfish.CURRENT ANALYTICAL CHEMISTRY,19(9),677-684.
MLA	Ullah, Najeeb,et al."A Novel Fluorescent Aptasensor Based on Mesoporous Silica Nanoparticles for Selective and Sensitive Detection of Saxitoxin in Shellfish".CURRENT ANALYTICAL CHEMISTRY 19.9(2023):677-684.