Synergistic Amelioration of Osseointegration and Osteoimmunomodulation with a Microarc Oxidation-Treated Three-Dimensionally Printed Ti-24Nb-4Zr-8Sn Scaffold via Surface Activity and Low Elastic Modulus

doi:10.1021/acsami.3c16459

IMR OpenIR

	Synergistic Amelioration of Osseointegration and Osteoimmunomodulation with a Microarc Oxidation-Treated Three-Dimensionally Printed Ti-24Nb-4Zr-8Sn Scaffold via Surface Activity and Low Elastic Modulus
	Yang, Xinyue 1; Wu, Lijun 2; Li, Cheng 1; Li, Shujun 3; Hou, Wentao 3; Hao, Yulin 3; Lu, Yiping 2; Li, Lei 1
通讯作者	Hao, Yulin(ylhao@imr.ac.cn) ; Lu, Yiping(luyiping@dlut.edu.cn) ; Li, Lei(spinecmull@163.com)
	2024-01-11
发表期刊	ACS APPLIED MATERIALS & INTERFACES
ISSN	1944-8244
卷号	16 期号:3 页码:3171-3186
摘要	Biomaterial scaffolds, including bone substitutes, have evolved from being primarily a biologically passive structural element to one in which material properties such as surface topography and chemistry actively direct bone regeneration by influencing stem cells and the immune microenvironment. Ti-6Al-4V-(Ti6Al4V) implants, with a significantly higher elastic modulus than human bone, may lead to stress shielding, necessitating improved stability at the bone-titanium alloy implant interface. Ti-24Nb-4Zr-8Sn (Ti2448), a low elastic modulus beta-type titanium alloy devoid of potentially toxic elements, was utilized in this study. We employed 3D printing technology to fabricate a porous scaffold structure to further decrease the structural stiffness of the implant to approximate that of cancellous bone. Microarc oxidation (MAO) surface modification technology is then employed to create a microporous structure and a hydrophilic oxide ceramic layer on the surface and interior of the scaffold. In vitro studies demonstrated that MAO treatment enhances the proliferation, adhesion, and osteogenesis capabilities on the scaffold surface. The chemical composition of the MAO-Ti2448 oxide layer is found to enhance the transcription and expression of osteogenic genes in bone mesenchymal stem cells (BMSCs), potentially related to the enrichment of Nb2O5 and SnO2 in the oxide layer. The MAO-Ti2448 scaffold, with its synergistic surface activity and low stiffness, significantly activates the anti-inflammatory macrophage phenotype, creating an immune microenvironment that promotes the osteogenic differentiation of BMSCs. In vivo experiments in a rabbit model demonstrated a significant improvement in the quantity and quality of the newly formed bone trabeculae within the scaffold under the contact osteogenesis pattern with a matched elastic modulus. These trabeculae exhibit robust connections to the external structure of the scaffold, accelerating the formation of an interlocking structure between the bone and implant and providing higher implantation stability. These findings suggest that the MAO-Ti2448 scaffold has significant potential as a bone defect repair material by regulating osteoimmunomodulation and osteogenesis to enhance osseointegration. This study demonstrates an optional strategy that combines the mechanism of reducing the elastic modulus with surface modification treatment, thereby extending the application scope of beta-type titanium alloy.
关键词	3D printing Ti2448 osteoimmunomodulation low elastic modulus microarc oxidation contactosteogenesis
资助者	Natural Science Foundation of Liaoning Province ; Science and Technology Department of Liaoning Province
DOI	10.1021/acsami.3c16459
收录类别	SCI
语种	英语
资助项目	Natural Science Foundation of Liaoning Province ; Science and Technology Department of Liaoning Province[2022JH1/10800073]
WOS研究方向	Science & Technology - Other Topics ; Materials Science
WOS类目	Nanoscience & Nanotechnology ; Materials Science, Multidisciplinary
WOS记录号	WOS:001150616800001
出版者	AMER CHEMICAL SOC
引用统计	被引频次：10[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.imr.ac.cn/handle/321006/183724
专题	中国科学院金属研究所
通讯作者	Hao, Yulin; Lu, Yiping; Li, Lei
作者单位	1.China Med Univ, Dept Orthopaed Surg, Shengjing Hosp, Shenyang 110055, Liaoning, Peoples R China 2.Dalian Univ Technol, Engn Res Ctr High Entropy Alloy Mat Liaoning Prov, Sch Mat Sci & Engn, Dalian 116024, Peoples R China 3.Chinese Acad Sci, Inst Met Res, Shenyang 110016, Peoples R China
推荐引用方式 GB/T 7714	Yang, Xinyue,Wu, Lijun,Li, Cheng,et al. Synergistic Amelioration of Osseointegration and Osteoimmunomodulation with a Microarc Oxidation-Treated Three-Dimensionally Printed Ti-24Nb-4Zr-8Sn Scaffold via Surface Activity and Low Elastic Modulus[J]. ACS APPLIED MATERIALS & INTERFACES,2024,16(3):3171-3186.
APA	Yang, Xinyue.,Wu, Lijun.,Li, Cheng.,Li, Shujun.,Hou, Wentao.,...&Li, Lei.(2024).Synergistic Amelioration of Osseointegration and Osteoimmunomodulation with a Microarc Oxidation-Treated Three-Dimensionally Printed Ti-24Nb-4Zr-8Sn Scaffold via Surface Activity and Low Elastic Modulus.ACS APPLIED MATERIALS & INTERFACES,16(3),3171-3186.
MLA	Yang, Xinyue,et al."Synergistic Amelioration of Osseointegration and Osteoimmunomodulation with a Microarc Oxidation-Treated Three-Dimensionally Printed Ti-24Nb-4Zr-8Sn Scaffold via Surface Activity and Low Elastic Modulus".ACS APPLIED MATERIALS & INTERFACES 16.3(2024):3171-3186.